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Associations of biochemical changes and maternal traits with mutation 1843 (C>T) in the RYR1 gene as a common cause for porcine stress syndrome Cover

Associations of biochemical changes and maternal traits with mutation 1843 (C>T) in the RYR1 gene as a common cause for porcine stress syndrome

Open Access
|Dec 2016

Figures & Tables

Thirteen percent polyacrylamide gel electrophoresis (PAGE) for PSS genotyping of the digested fragment of interest from the ryr1 gene was done by RFLP using the HhaI restriction enzyme.Lane 1: heterozygous (Nn genotype);lane 2: homozygous (nn genotype); L: low range DNA ladder;lane 3: normal (NN genotype).

Biochemical data of plasma samples from animals with different porcine stress syndrome genotypes_

Biochemical ParameterGenotypeReference Values

Reference values from the Research Animal Resources, University of Minnesota, Minneapolis, MN, USA (www.ahc.umn.edu/rar/refvalues.htm).

t Value
NNNnnn
Ca2+ (µmol/L)2.622.682.712.50-2.951.14

A p value of >0.05 was not considered to be significant.

Creatinine (µmol/L)142.10157.30174.6088.40-265.209.97

A p value of <0.05 was considered to be significant.

Total proteins (g/L)72.4075.7074.9068.00-89.001.42

A p value of >0.05 was not considered to be significant.

Bilirubin (µmol/L)1.652.544.120.00-12.0012.79

A p value of <0.05 was considered to be significant.

AP (U/L)21.8045.3062.409.00-70.0014.55

A p value of <0.05 was considered to be significant.

AST (U/L)29.2058.30125.5017.00-45.0016.32

A p value of <0.05 was considered to be significant.

ALT (U/L)35.3051.7048.209.00-60.009.14

A p value of >0.05 was not considered to be significant.

LDH (U/L398.00655.00894.00159.00-475.0017.02

A p value of <0.05 was considered to be significant.

CPK (U/L)276.00591.00764.0067.00-489.0015.29

A p value of <0.05 was considered to be significant.

Association of the porcine stress syndrome genotype with maternal traits (newborn, stillborn and weaned piglets)_

PSS GenotypeNewborn Pigletst Value
NN10.920.22
Nn11.730.522.59

A p value of <0.05 was considered to be significant.

nn11.501.50
Stillborn Piglets
NN0.150.06
Nn0.090.092.53

A p value of <0.05 was considered to be significant.

nn1.50.50
Weaned Piglets
NN8.200.19
Nn9.000.460.05

A p value of >0.05 was not considered to be significant.

nn7.500.50
Language: English
Page range: 75 - 80
Published on: Dec 30, 2016
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year

© 2016 ZT Popovski, B Tanaskovska, E Miskoska-Milevska, S Andonov, S Domazetovska, published by Macedonian Academy of Sciences and Arts
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.