Thirteen percent polyacrylamide gel electrophoresis (PAGE) for PSS genotyping of the digested fragment of interest from the ryr1 gene was done by RFLP using the HhaI restriction enzyme.
Lane 1: heterozygous (Nn genotype);
lane 2: homozygous (nn genotype); L: low range DNA ladder;
lane 3: normal (NN genotype).
Table 1
Biochemical data of plasma samples from animals with different porcine stress syndrome genotypes.
| Biochemical Parameter | Genotype | Reference Valuesa | t Value | ||
|---|---|---|---|---|---|
| NN | Nn | nn | |||
| Ca2+ (µmol/L) | 2.62 | 2.68 | 2.71 | 2.50-2.95 | 1.14b |
| Creatinine (µmol/L) | 142.10 | 157.30 | 174.60 | 88.40-265.20 | 9.97c |
| Total proteins (g/L) | 72.40 | 75.70 | 74.90 | 68.00-89.00 | 1.42b |
| Bilirubin (µmol/L) | 1.65 | 2.54 | 4.12 | 0.00-12.00 | 12.79c |
| AP (U/L) | 21.80 | 45.30 | 62.40 | 9.00-70.00 | 14.55c |
| AST (U/L) | 29.20 | 58.30 | 125.50 | 17.00-45.00 | 16.32c |
| ALT (U/L) | 35.30 | 51.70 | 48.20 | 9.00-60.00 | 9.14b |
| LDH (U/L | 398.00 | 655.00 | 894.00 | 159.00-475.00 | 17.02c |
| CPK (U/L) | 276.00 | 591.00 | 764.00 | 67.00-489.00 | 15.29c |
NN: stress-free; Nn: heterozygous carrier; nn: homozygous carrier; Ca2+: calcium ion; AP: alkaline phosphatase; AST: aspartate aminotransferase; ALT: alanine aminotransferase; LDH: lactate dehydrogenase; CPK: creatine phosphokinase.
a Reference values from the Research Animal Resources, University of Minnesota, Minneapolis, MN, USA (www.ahc.umn.edu/rar/refvalues.htm).
Table 2
Association of the porcine stress syndrome genotype with maternal traits (newborn, stillborn and weaned piglets).
| PSS Genotype | Newborn Piglets | t Value | |
|---|---|---|---|
| NN | 10.92 | 0.22 | |
| Nn | 11.73 | 0.52 | 2.59a |
| nn | 11.50 | 1.50 | |
| Stillborn Piglets | |||
| NN | 0.15 | 0.06 | |
| Nn | 0.09 | 0.09 | 2.53a |
| nn | 1.5 | 0.50 | |
| Weaned Piglets | |||
| NN | 8.20 | 0.19 | |
| Nn | 9.00 | 0.46 | 0.05b |
| nn | 7.50 | 0.50 | |
NN: stress-free; Nn: heterozygous carrier; nn: homozygous carrier.