Figure 1
Figure 2
Figure 3
Figure 4
![Three-dimensional structure of glucose-6-phosphate dehydrogenase (G6PD)Viangchan obtained through mutation in silico. The wild-type G6PD structure (PDBID: 2HB9) was retrieved from the RCSB Protein Data Bank and subjected to mutation in silico using the UCSF Chimera Rotamer tool [25]. Valine 291 was changed to methionine (in red) (G6PDViangchan). The side chain rotamer of the mutated residue was selected based on the Dynameomics rotamers library [26]. The energy minimization was performed on the G6PDViangchan variant (V291M) using the Gromacs simulation package (version 4.6.7) [27]. The molecular docking was performed using AutoDock (version 4.2) [28]. The yellow region represents the structural nicotinamide adenine dinucleotide phosphate (NADP+) binding site and green region represents substrate binding site.](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/647067f983f1392090d68d7d/j_abm-2020-0023_fig_004.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=ASIA6AP2G7AKGIU4YTSG%2F20260314%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20260314T191206Z&X-Amz-Expires=3600&X-Amz-Security-Token=IQoJb3JpZ2luX2VjEOH%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaDGV1LWNlbnRyYWwtMSJIMEYCIQCb2JTHpmlz2w13TdFvM0vhdnTXC8wCXWCURvOw7cWf9QIhAOsPEoHnH1mm3fsAsd5DXZbpLGAzOK36fg2IsvmVuaQJKsQFCKr%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEQAhoMOTYzMTM0Mjg5OTQwIgz%2FI9Emb8cZL0XuNUcqmAWQ%2FxDcx1s%2FoWMO0MvSujvSN%2BS9T8BOTGm3zvmcaMRZKhfTl%2FyAohsYdI0XUjIXFq1ju8%2B7nIkqIteX61%2Fvb7qKfIMDI1h5pEGm6s9GLZ5ZCqZjca1eDu49RziWhvSv40pegRAewl7ghl3CxpIePpGh6gkkG6BSiOCa53%2BcsjLf64UC1lwVl8qnl9Tz5cMjpk7ZBqwP9pZDkr7DLI9S1yJrCnw8R6X9oBtGn1aKJ5PsnejIxMIjmw0SuQlLAjjh1gMrSLXy%2FURhLVXMQcvKHbcv23Uz8BqXl0j0tIZmbO0iuIloukUHDIwLrYLXWdQYL2mE9bSagM1bwrmFFRynfiVbYNp4G0OeNMlKxneWvm%2Fl1qlasa6jW12uLxTjM9A8SX7nAHcYFsI%2FxIQM9FufGP7ASYyxYxDUG5FoGZ7m5pOUjypi%2FrjGsfM%2BMrQ7KyMyDynRkFmkb8zRJl%2F7nvNLTz3devTC3ygjC6nxLUCGx%2B9fX3tOUwPBF1J45pmeyMOPx3PjSDDfelmErM8b0tgb%2BA0Z3VDMyDnWr%2BOiVW4r9GWwXfwMzv1jbmCdwijHKy29FAzYU3tZUDVwJwHU6NRT8Anqhfmo25zM2Eo3%2B31YzXTXnoP2bOawb6AiVEeJ6VOPV2uNws6hJiAKeSS3iVIMq3CUZ7t17GnibA3oFVmZP5146LLu8UGVSd6zlyAmGRQyxp3lzfbGY64CVyinbuUiduMvDTtEoAkl5aH0xvTCJ6qvagMhHp9iGWmeDupBSMOCC7nTSosvjcBdOol8dil%2BBt%2FzDZec9r1sSAXFklpFd%2Bvl19YTJdStUEHE%2Brwy5a7VvW8Q1XFRZauAWqNtNKVXEVVtqi7EqS9qK16ipCtlkZKUAd57GQAhav7fMJGw1s0GOrABMvEvDy%2B72GJeuVUp3tCkWCUlZWqCWKXHuill0nbyBTwz2o2cKH3fzc60dJQ63XOw6uZCXkDxOW1pZ5AKY%2BjTf44WhLzBdEoqtWO3vmTgYRGKIQEXpzhylOiJ5gXQI8GNUbF7tyDjeT9uSKF5Y75W79lexXeDXGeU2Ar5Kvm6txYbw1K8IDiNbb5%2Biw5pHq7CRIqyMGUjn0V2z31JkDQ%2BkwMnMWAwNJk1ZxGvCxUaMLI%3D&X-Amz-Signature=77e297309d9d394e9dcd3bd0105578834f21e2a389670bcca2cf9af992d10ab4&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Specific enzyme activity (U/mg) of heterologous glucose-6-phosphate dehydrogenase
| Variant | Roos et al. [18] | Wang et al. [20] | Huang et al. [19] | Wang and Engel [21] | Gómez-Manzo et al. [33] | Gómez-Manzo et al. [17] | Gómez-Manzo et al. [23] | Boonyuen et al. [22] |
|---|---|---|---|---|---|---|---|---|
| Wild-type G6PD | 210 | 180 | 182 | 210 | 224 | 224 | 230 | 228 |
| Amsterdam | – | – | – | 95 | – | – | – | – |
| Volendam | 36 | – | – | – | – | – | – | – |
| Wisconsin | – | – | – | – | 178 | – | – | – |
| Nashville | – | 130 | – | – | – | 103 | – | – |
| Yucatan | – | – | – | – | – | 132 | – | – |
| Valladolid | – | – | – | – | – | 92 | – | – |
| Mexico City | – | – | – | – | – | 175 | – | – |
| Mahidol | – | – | 177 | – | – | – | – | 141 |
| Fukaya | – | 175 | – | – | – | – | – | – |
| Champinas | – | 160 | – | – | – | – | – | – |
| Plymouth | – | – | 187 | – | – | – | – | – |
| Viangchan | – | – | – | – | – | – | 228 | 107 |
| Zacatecas | – | – | – | – | – | – | 58 | – |
| Vanua-Lava | – | – | – | – | – | – | 182 | – |