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Variation in contents of active components and antibacterial activity in different parts of Lonicera japonica Thunb Cover

Variation in contents of active components and antibacterial activity in different parts of Lonicera japonica Thunb

Open Access
|Jul 2020

Figures & Tables

Figure 1

Representative high-performance liquid chromatograms of standard mixture (A) and sample (B) of honeysuckle leaves. Peaks identification: 1, neochlorogenic acid; 2, chlorogenic acid; 3, caffeic acid; 4, rutin; 5, galuteolin; 6, hyperoside; 7, isochlorogenic acid C; 8, luteolin; and 9, quercetin. Chromatographic conditions are as depicted in the text.
Representative high-performance liquid chromatograms of standard mixture (A) and sample (B) of honeysuckle leaves. Peaks identification: 1, neochlorogenic acid; 2, chlorogenic acid; 3, caffeic acid; 4, rutin; 5, galuteolin; 6, hyperoside; 7, isochlorogenic acid C; 8, luteolin; and 9, quercetin. Chromatographic conditions are as depicted in the text.

Figure 2

Total ion chromatogram of the volatile components in June in leaves (LE) (A), flowers (FL) (B), and green buds (GB) (C) of L. japonica Thunb. The chromatographic and mass spectrometric conditions are as depicted in the text. The LE were collected in June 2015 with FL and GB.
Total ion chromatogram of the volatile components in June in leaves (LE) (A), flowers (FL) (B), and green buds (GB) (C) of L. japonica Thunb. The chromatographic and mass spectrometric conditions are as depicted in the text. The LE were collected in June 2015 with FL and GB.

Figure 3

The antibacterial effect on Staphylococcus aureus. (A) Leaves (LE) were collected in June, (B) LE were collected in July, (C) green buds (GB), and (D) flowers (FL). The FL and GB were collected in June 2015.
The antibacterial effect on Staphylococcus aureus. (A) Leaves (LE) were collected in June, (B) LE were collected in July, (C) green buds (GB), and (D) flowers (FL). The FL and GB were collected in June 2015.

The contents of nine components in L_ japonica Thunb leaves (LE), green buds (GB), and flowers (FL)

CompoundContent/mg/g
LE*GBFL
Galuteolin1.800.030.045
Neochlorogenic acid0.550.510.40
Chlorogenic acid17.7524.9116.96
Isochlorogenic acid C140.47112.20120.09
Caffeic acid0.0110.030.018
Rutin13.820.952.50
Hyperoside
Luteolin0.170.0660.074
Quercetin

Total flavonoids in L_ japonica Thunb leaves (LE), green buds (GB), and flowers (FL) (n = 3)

LE*GBFL
Content/%6.687.386.35
RSD %0.630.411.42

Bacteriostatic results of L_ japonica Thunb leaves (LE), green buds (GB), and flowers (FL)

SamplesConcentration (g/mL)Diameters of the inhibition zone (mm)
SARSD/%ESBL-SARSD/%ECESBL-EC
LE*1.0130.78140.74
GB1.0111.17130.43
FL1.0120.45111.30

Volatile components in L_ japonica Thunb leaves (LE), green buds (GB), and flowers (FL)

CompoundContent/%*
LEGBFL
11,4-Eicosadiene10.940.470.42
2Tricosane0.500.50
3(Z)-9-Octadecenamide0.37
43-Ethyl-5-(2-ethylbutyl)-octadecane1.42
5Heptacosane6.707.59
6Tetratriacontane6.794.79
7Nonacosane10.1130.7132.82
8Tetratetracontane0.62
9Triacontane8.3314.6019.67
1017-Pentatriacontene17.677.247.24
11Squalene8.720.520.66
12Pentacosane1.85
13Hexadecanamide
149-Octadecene,1,1-[1,2-ethanediylbis(oxy)] bis-(Z,Z)-
Hydrocarbons/%66.0767.9470.75
152-Heptadecanone1.491.59
16Bacteriochlorophyll-c-stearyl0.41
176,10,14-Trimethyl-2-pentadecanone0.17
182-Nonadecanone0.33
19Phytol1.851.900.52
20(Z)-Ethanol, 2-(9-octadecenyloxy)-0.20
21γ-Sitosterol6.926.588.35
22(Z,Z,Z)-9,12,15-Octadecatrien-1-ol
23Stigmasterol
24Trans-Geranylgeraniol0.37
2514,16-Hentriacontanedione6.74
Alcohol ketones/%9.3510.5017.57
26n-Hexadecanoic acid0.753.76
27Phytol, acetate3.561.361.13
28(Z,Z,Z)-9,12,15-Octadecatrienoic acid0.740.29
29(Z)-13-Docosenamide6.341.41
Acids/%10.642.404.89
3015-Isobutyl-(13αH)-isocopalane1.46
318,14-Seco-3,19-epoxyandrostane-8,14-di-one, 17-acetoxy-3β-methoxy-4,4-dimethyl-0.39
32(3β,24Z)-Stigmasta-5,24(28)-dien-3-ol
33(3β)-9,19-Cyclolanost-24-en-3-ol13.5110.69
Terpenes/%15.3610.690
34Docosanoic acid, methyl ester0.41
35(Z,Z,Z)-9,12,15-Octadecatrienoic acid, methyl ester
36Tetracosanoic acid, methyl ester3.811.72
37Hexacosanoic acid, methyl ester1.260.72
38Hexadecanoic acid, methyl ester0.410.79
39Heptanoic acid, docosyl ester
40(Z,Z)-9-Hexadecenoic acid, 9-octadecenyl ester0.24
41Oleic acid, 3-(octadecyloxy)propyl ester
42Oleic acid, eicosyl ester0.37
Lipids%0.785.723.23
432,2-Methylenebis[6-(1,1-dimethylethyl)-4-methyl-phenol]1.42
44Vitamin E2.422.031.51
Derivatives/%3.842.031.51
DOI: https://doi.org/10.1515/abm-2020-0004 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
Language: English
Page range: 19 - 26
Published on: Jul 13, 2020
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year

© 2020 Luyun Yan, Yulei Xie, Youyou Wang, Jinhua Zhu, Mingjing Li, Xiuhua Liu, Dongbao Zhao, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.