Skip to main content
Have a personal or library account? Click to login
Osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells encapsulated in Thai silk fibroin/collagen hydrogel: a pilot study in vitro Cover

Osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells encapsulated in Thai silk fibroin/collagen hydrogel: a pilot study in vitro

Asian Biomedicine
Volume 12 (2018): Issue 6 (December 2018)
By: Jirun Apinun,  Sittisak Honsawek,  Somsak Kuptniratsaikul,  Jutarat Jamkratoke and  Sorada Kanokpanont  
Open Access
|Oct 2019
Figures & tables

Figures & Tables

Figure 1

Growth kinetics of rat mesenchymal stem cells cultured in hydrogels. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). Error bars are standard deviations of triplicate experiments.

Figure 2

DNA assay of rat mesenchymal stem cells cultured in hydrogels in osteogenic induction medium. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). *P < 0.05 (one-way analysis of variance with a post hoc least significant difference test). Error bars are standard deviations of triplicate experiments.

Figure 3

Alkaline phosphatase (ALP) activity of rat mesenchymal stem cells cultured on hydrogels in osteogenic induction medium. Units indicate mM p-nitrophenol produced per min per cell. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). Error bars are standard deviations of triplicate experiments.

Figure 4

Calcium content of rat mesenchymal stem cells cultured on hydrogels in osteogenic induction medium. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). *P < 0.05 (one-way analysis of variance with a post hoc least significant difference test). Error bars are standard deviations of triplicate experiments.

Figure 5

SEM images of rat MSCs cultured in hydrogels under different magnifications. SF (hydrogel made of 4% silk fibroin (SF) solution only, SF/0.05C (hydrogel made of 4% SF solution + 0.05% collagen solution), and SF/0.1C (hydrogel made of 4% SF solution + 0.1% collagen solution).

Elemental analysis result by EDS

HydrogelsC (% wt)O (% wt)Ca (% wt)P (% wt)Ca/P
SF45.03 ± 5.5637.61 ± 3.7513.99 ± 1.684.03 ± 1.814.28 ± 2.38
SF/0.05C39.36 ± 2.9633.99 ± 10.5619.27 ± 7.137.38 ± 3.372.91 ± 1.09
SF/0.1C45.92 ± 10.0038.96 ± 3.1110.86 ± 6.044.27 ± 4.373.61 ± 1.75
DOI: https://doi.org/10.1515/abm-2019-0030 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
Journal RSS Feed
Language: English
Page range: 273 - 279
Published on: Oct 24, 2019
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year
Keywords:
collagen,
fibroins,
hydrogels,
mesenchymal stem cells,
silk
Related subjects:
Medicine,
Assistive professions, nursing,
Basic medical science,
Basic medical science, other,
Clinical medicine,
Clinical medicine, other

© 2019 Jirun Apinun, Sittisak Honsawek, Somsak Kuptniratsaikul, Jutarat Jamkratoke, Sorada Kanokpanont, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 12 (2018): Issue 6 (December 2018)
Previous article
Next article