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Osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells encapsulated in Thai silk fibroin/collagen hydrogel: a pilot study in vitro Cover

Osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells encapsulated in Thai silk fibroin/collagen hydrogel: a pilot study in vitro

Asian Biomedicine
Volume 12 (2018): Issue 6 (December 2018)
By: Jirun Apinun,  Sittisak Honsawek,  Somsak Kuptniratsaikul,  Jutarat Jamkratoke and  Sorada Kanokpanont  
Open Access
|Oct 2019

Figures & Tables

Figure 1

Growth kinetics of rat mesenchymal stem cells cultured in hydrogels. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). Error bars are standard deviations of triplicate experiments.
Growth kinetics of rat mesenchymal stem cells cultured in hydrogels. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). Error bars are standard deviations of triplicate experiments.

Figure 2

DNA assay of rat mesenchymal stem cells cultured in hydrogels in osteogenic induction medium. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). *P < 0.05 (one-way analysis of variance with a post hoc least significant difference test). Error bars are standard deviations of triplicate experiments.
DNA assay of rat mesenchymal stem cells cultured in hydrogels in osteogenic induction medium. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). *P < 0.05 (one-way analysis of variance with a post hoc least significant difference test). Error bars are standard deviations of triplicate experiments.

Figure 3

Alkaline phosphatase (ALP) activity of rat mesenchymal stem cells cultured on hydrogels in osteogenic induction medium. Units indicate mM p-nitrophenol produced per min per cell. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). Error bars are standard deviations of triplicate experiments.
Alkaline phosphatase (ALP) activity of rat mesenchymal stem cells cultured on hydrogels in osteogenic induction medium. Units indicate mM p-nitrophenol produced per min per cell. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). Error bars are standard deviations of triplicate experiments.

Figure 4

Calcium content of rat mesenchymal stem cells cultured on hydrogels in osteogenic induction medium. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). *P < 0.05 (one-way analysis of variance with a post hoc least significant difference test). Error bars are standard deviations of triplicate experiments.
Calcium content of rat mesenchymal stem cells cultured on hydrogels in osteogenic induction medium. Squares indicate hydrogels made of 4% silk fibroin (SF) solution only (SF hydrogel), circles indicate hydrogels made of 4% SF solution + 0.05% collagen solution (SF/0.05C hydrogel), and triangles indicate hydrogels made of 4% SF solution + 0.1% collagen solution (SF/0.1C hydrogel). *P < 0.05 (one-way analysis of variance with a post hoc least significant difference test). Error bars are standard deviations of triplicate experiments.

Figure 5

SEM images of rat MSCs cultured in hydrogels under different magnifications. SF (hydrogel made of 4% silk fibroin (SF) solution only, SF/0.05C (hydrogel made of 4% SF solution + 0.05% collagen solution), and SF/0.1C (hydrogel made of 4% SF solution + 0.1% collagen solution).
SEM images of rat MSCs cultured in hydrogels under different magnifications. SF (hydrogel made of 4% silk fibroin (SF) solution only, SF/0.05C (hydrogel made of 4% SF solution + 0.05% collagen solution), and SF/0.1C (hydrogel made of 4% SF solution + 0.1% collagen solution).

Elemental analysis result by EDS

HydrogelsC (% wt)O (% wt)Ca (% wt)P (% wt)Ca/P
SF45.03 ± 5.5637.61 ± 3.7513.99 ± 1.684.03 ± 1.814.28 ± 2.38
SF/0.05C39.36 ± 2.9633.99 ± 10.5619.27 ± 7.137.38 ± 3.372.91 ± 1.09
SF/0.1C45.92 ± 10.0038.96 ± 3.1110.86 ± 6.044.27 ± 4.373.61 ± 1.75
DOI: https://doi.org/10.1515/abm-2019-0030 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
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Language: English
Page range: 273 - 279
Published on: Oct 24, 2019
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year
Keywords:
collagen,
fibroins,
hydrogels,
mesenchymal stem cells,
silk
Related subjects:
Medicine,
Assistive professions, nursing,
Basic medical science,
Basic medical science, other,
Clinical medicine,
Clinical medicine, other

© 2019 Jirun Apinun, Sittisak Honsawek, Somsak Kuptniratsaikul, Jutarat Jamkratoke, Sorada Kanokpanont, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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