Figure 1
Genetically engineered pigs as organ sources for xenotransplantation. Using gene disruption and transgenic technologies, juvenile and adult pigs have been engineered and serve as organ sources for xenotransplantation.
Figure 2
Exogenic organs using blastocyst complementation. Schematic highlighting the strategy for engineering exogenic, human organs in gene edited pigs. In this strategy, an entire lineage is deleted and the null porcine embryo is complemented by human induced pluripotent stem cells (iPSCs), which populate the vacant niche producing a human organ (ie, heart). Such a strategy could be used to engineer individual exogenic organs (from the patient’s hiPSCs) or an off-the shelf (human leukocyte antigens compatible hiPSCs) version.