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Transient impedance changes in venous endothelial monolayers as a biological radiation dosimetry response Cover

Transient impedance changes in venous endothelial monolayers as a biological radiation dosimetry response

Journal of Electrical Bioimpedance
Volume 3 (2012): Issue 1 (January 2012)
By: Erik F. Young  
Open Access
|Oct 2012

Figures & Tables

Fig.1

Radiation induced changes in venous endothelial monolayer permeability via ECIS. Primary human umbilical cord vein endothelial cells (HUVECs) were seeded on collagen coated 8W10E+ arrays and allowed to mature into competent monolayers prior to irradiation. Transmonolayer resistance for the entire course of culture is shown in A. The trace shows contact, spreading, sealing and decline of the monolayer’s cells over time. Changes in resistance after irradiation shown in B reveal a significant decrease (**, p = 0.017) in monolayer resistance 3 h after irradiation. The dominant contributor to this resistance is the modeled Rb parameter (C) that reported a statistically significant (*, p = 0.038) change in resistance arising from lateral cell contacts. The mean and standard deviation of three monolayers is plotted. Radiation induces an early, transient decrease in endothelial monolayer permeability.
Radiation induced changes in venous endothelial monolayer permeability via ECIS. Primary human umbilical cord vein endothelial cells (HUVECs) were seeded on collagen coated 8W10E+ arrays and allowed to mature into competent monolayers prior to irradiation. Transmonolayer resistance for the entire course of culture is shown in A. The trace shows contact, spreading, sealing and decline of the monolayer’s cells over time. Changes in resistance after irradiation shown in B reveal a significant decrease (**, p = 0.017) in monolayer resistance 3 h after irradiation. The dominant contributor to this resistance is the modeled Rb parameter (C) that reported a statistically significant (*, p = 0.038) change in resistance arising from lateral cell contacts. The mean and standard deviation of three monolayers is plotted. Radiation induces an early, transient decrease in endothelial monolayer permeability.

Fig.2

Time-lapse video microscopy of irradiated primary HUVECs. Cells were seeded on collagen-coated substrate and allowed to mature into confluent monolayers before irradiation with 5.0 Gy of γ rays. Frames from the video timecourse (Supplementary Movies S2,S3) were captured after irradiation at the intervals shown. Endothelial monolayers are dynamic with dividing and motile cells and are not overtly compromised after radiation. Scale bars; 200 μ.
Time-lapse video microscopy of irradiated primary HUVECs. Cells were seeded on collagen-coated substrate and allowed to mature into confluent monolayers before irradiation with 5.0 Gy of γ rays. Frames from the video timecourse (Supplementary Movies S2,S3) were captured after irradiation at the intervals shown. Endothelial monolayers are dynamic with dividing and motile cells and are not overtly compromised after radiation. Scale bars; 200 μ.
DOI: https://doi.org/10.5617/jeb.395 | Journal eISSN: 1891-5469
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Language: English
Page range: 61 - 65
Submitted on: Jul 26, 2012
Published on: Oct 23, 2012
Published by: University of Oslo
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Bioimpedance,
endothelium,
ECIS,
radiation,
dosimetry,
HUVEC
Related subjects:
Engineering,
Bioengineering and biomedical engineering,
Biomedical electronics,
Life sciences,
Biophysics,
Medicine,
Biomedical engineering,
Physics,
Spectroscopy and metrology

© 2012 Erik F. Young, published by University of Oslo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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