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Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz Cover

Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz

Journal of Electrical Bioimpedance
Volume 2 (2011): Issue 1 (January 2011)
By: Colin Chung,  Martin Waterfall,  Steve Pells,  Anoop Menachery,  Stewart Smith and  Ronald Pethig  
Open Access
|Sep 2011

Figures & Tables

Fig.1

Frequency variation of the real part of the Clausius-Mossotti function for lymphocytes, derived from published dielectric data [5]. The DEP force is proportional to this function and shows two cross-over frequencies, fxo1 and fxo2.
Frequency variation of the real part of the Clausius-Mossotti function for lymphocytes, derived from published dielectric data [5]. The DEP force is proportional to this function and shows two cross-over frequencies, fxo1 and fxo2.

Fig.2

Schematic of the electrical system used to investigate the DEP properties of cells above 100 MHz.
Schematic of the electrical system used to investigate the DEP properties of cells above 100 MHz.

Fig.3

Interdigitated electrode fingers and example current path.
Interdigitated electrode fingers and example current path.

Fig.4

Distributed RCR interdigitated electrode model.
Distributed RCR interdigitated electrode model.

Fig.5

Voltage difference between adjacent fingers (Design 1).
Voltage difference between adjacent fingers (Design 1).

Fig.6

Voltage difference between adjacent fingers (Design 2).
Voltage difference between adjacent fingers (Design 2).

Fig.7

Fxo2 spectrum for SP2/O cells (n=418) suspended in DEP medium with 310 mOsm/L osmolality and 33 mS/m conductivity.
Fxo2 spectrum for SP2/O cells (n=418) suspended in DEP medium with 310 mOsm/L osmolality and 33 mS/m conductivity.

Fig.8

Mean fxo2 frequency for SP2/O cells suspended in DEP medium with 310 mOsm/L osmolality and 33 mS/m conductivity over 6 hours. Single standard deviation bars shown.
Mean fxo2 frequency for SP2/O cells suspended in DEP medium with 310 mOsm/L osmolality and 33 mS/m conductivity over 6 hours. Single standard deviation bars shown.

Fig.9

Mean fxo2 frequency for SP2/O cells suspended in 310 mOsm DEP medium at 10°C, 21°C, 29°C and 37°C over 6 hours. Single standard deviation bars are shown.
Mean fxo2 frequency for SP2/O cells suspended in 310 mOsm DEP medium at 10°C, 21°C, 29°C and 37°C over 6 hours. Single standard deviation bars are shown.

Fig.10

Mean fxo2 frequency for SP2/O cells suspended in DEP media of 250, 310, 390, 425 and 480 mOsm/L. Standard deviation bars are shown.
Mean fxo2 frequency for SP2/O cells suspended in DEP media of 250, 310, 390, 425 and 480 mOsm/L. Standard deviation bars are shown.

Fig.11

Arrhenius plot for the initial rate of mean fxo2 roll-off.
Arrhenius plot for the initial rate of mean fxo2 roll-off.

Fig.A1

Effective conductivity and relative permittivity of a cell based on the dielectric data of Asami et al [5]. The dark band indicates where real solutions for the DEP cross-over do not exist.
Effective conductivity and relative permittivity of a cell based on the dielectric data of Asami et al [5]. The dark band indicates where real solutions for the DEP cross-over do not exist.

j_jeb_196_tab_003_w2aab3b7b7b1b6b1ab1b6ab2Aa

Radius (r) / Thickness (t)Complex Permittivity
Nucleoplasmrnpεnp*$\varepsilon _{np}^{*}$
Nuclear Envelopetneεne*$\varepsilon _{ne}^{*}$
Cytoplasmrcpεcp*$\varepsilon _{cp}^{*}$
Cell Membranetmbεmb*$\varepsilon _{mb}^{*}$

Model parameters for two electrode design examples_

Design 1Design 2
Number of Fingers1616
Finger Overlap (LO)202mm
Finger Width (LW)2025μm
Finger Spacing (LS)2035μm
Medium Conductivity3333mS/m
Sheet Resistance3.61.8Ω/□
Section Length (Δx)20.2mm
Section Medium Resistance (Rs)30340kΩ
Section Track Resistance (Rt)28.814.4Ω
Section Track Capacitance (C)150067fF
Area12.81.9mm2

Sensitivity of fxo2 to dielectric parameters based on Asami et al [5] for a nucleus volume fraction of 0_7_ The change in frequency for a ±10% variation in each parameter is normalised to the base fxo2 value of 195 MHz_

PermittivityConductivity
Medium31%0%
Nucleus8%13%
Cytoplasm6%1%
Nucl. Vol. Fraction8%
DOI: https://doi.org/10.5617/jeb.196 | Journal eISSN: 1891-5469
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Language: English
Page range: 64 - 71
Submitted on: Jul 22, 2011
Published on: Sep 7, 2011
Published by: University of Oslo
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Biodielectrics,
Cytoplasm Conductivity,
Dielectrophoresis,
Myeloma Cells,
Nucleus conductivity
Related subjects:
Engineering,
Bioengineering and biomedical engineering,
Biomedical electronics,
Life sciences,
Biophysics,
Medicine,
Biomedical engineering,
Physics,
Spectroscopy and metrology

© 2011 Colin Chung, Martin Waterfall, Steve Pells, Anoop Menachery, Stewart Smith, Ronald Pethig, published by University of Oslo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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