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Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in Bacteroides Species Cover

Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in Bacteroides Species

Polish Journal of Microbiology
Volume 67 (2018): Issue 1 (January 2018)
By: Isin Akyar,  Meltem Ayas,  Onur Karatuna and  Yesim Besli  
Open Access
|Feb 2022

Figures & Tables

Fig. 1.

Interpretation of the Carba NP test results: B. fragilis ATCC 25285 with no carbapenemase activity (left) and carbapenemase-producing B. fragilis strain TAL 2480 (right).The tubes denoted as “a” contain solution A + bacterial lysate, tubes “b” contain solution B (solution A + imipenem) + bacterial lysate. If acidification occurs as a result of hydrolysis of imipenem, the pH indicator (phenol red) causes a color change from red to yellow in tube b.
Interpretation of the Carba NP test results: B. fragilis ATCC 25285 with no carbapenemase activity (left) and carbapenemase-producing B. fragilis strain TAL 2480 (right).The tubes denoted as “a” contain solution A + bacterial lysate, tubes “b” contain solution B (solution A + imipenem) + bacterial lysate. If acidification occurs as a result of hydrolysis of imipenem, the pH indicator (phenol red) causes a color change from red to yellow in tube b.

Fig. 2.

The PCR amplification results for the cfiA gene in clinical Bacteroides spp. isolates.Lanes 1, 3 and 4 are clinical B. fragilis isolates demonstrating the presence of the cfiAX gene, lanes 2, 5–10 are clinical Bacteroides spp. lacking the cfia gene, lane 11 is negative control (water), lane 12 is a cfiA positive B. fragilis strain used as positive control. Molecular sizes in base pair (bp) are indicated at the left. (M: Molecular mass standard) This PCR assay was used to amplify a 302 bp fragment of the cfiA gene.
The PCR amplification results for the cfiA gene in clinical Bacteroides spp. isolates.Lanes 1, 3 and 4 are clinical B. fragilis isolates demonstrating the presence of the cfiAX gene, lanes 2, 5–10 are clinical Bacteroides spp. lacking the cfia gene, lane 11 is negative control (water), lane 12 is a cfiA positive B. fragilis strain used as positive control. Molecular sizes in base pair (bp) are indicated at the left. (M: Molecular mass standard) This PCR assay was used to amplify a 302 bp fragment of the cfiA gene.
DOI: https://doi.org/10.5604/01.3001.0011.6148 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 97 - 101
Submitted on: Oct 19, 2016
Accepted on: Dec 28, 2017
Published on: Feb 21, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
carbapenemase,
Carba NP,
imipenem
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Isin Akyar, Meltem Ayas, Onur Karatuna, Yesim Besli, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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