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Detection of Acinetobacter spp. in Blood Cultures by an Improved Fluorescent in Situ Hybridization Assay Cover

Detection of Acinetobacter spp. in Blood Cultures by an Improved Fluorescent in Situ Hybridization Assay

Polish Journal of Microbiology
Volume 67 (2018): Issue 1 (January 2018)
By: Hanieh Asaadi,  Behrouz Naeimi,  Somayyeh Gharibi,  Abdalnaser Khosravi,  Sina Dobaradaran,  Reza Taherkhani and  Saeed Tajbakhsh  
Open Access
|Feb 2022

Figures & Tables

Fig. 1.

Detection of Acinetobacter sp. in a blood culture specimen by FISH.Panels A, B, and C show the same specimen. (A) Shows red signal of Acinetobacter sp. cells, indicating hybridization with probe Aci-Cy3 (signal intensity 2+). (B) Indicates hybridization with probe ACA-Cy3 (signal intensity 1+). (C) Simultaneous hybridization with two probes Aci-Cy3 and ACA-Cy3 increased the fluorescent signal intensity of Acinetobacter sp. cells (signal intensity 3+) so that the detection of the bacterium was facilitated. Magnification, × 1000.
Detection of Acinetobacter sp. in a blood culture specimen by FISH.Panels A, B, and C show the same specimen. (A) Shows red signal of Acinetobacter sp. cells, indicating hybridization with probe Aci-Cy3 (signal intensity 2+). (B) Indicates hybridization with probe ACA-Cy3 (signal intensity 1+). (C) Simultaneous hybridization with two probes Aci-Cy3 and ACA-Cy3 increased the fluorescent signal intensity of Acinetobacter sp. cells (signal intensity 3+) so that the detection of the bacterium was facilitated. Magnification, × 1000.
DOI: https://doi.org/10.5604/01.3001.0011.6137 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 3 - 10
Published on: Feb 21, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
bacteremia,
blood culture,
FISH,
simultaneous hybridization
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Hanieh Asaadi, Behrouz Naeimi, Somayyeh Gharibi, Abdalnaser Khosravi, Sina Dobaradaran, Reza Taherkhani, Saeed Tajbakhsh, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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