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Prevalence of class 1 and 2 integrons among the multidrug resistant uropathogenic strains of Escherichia coli Cover

Prevalence of class 1 and 2 integrons among the multidrug resistant uropathogenic strains of Escherichia coli

Asian Biomedicine
Volume 9 (2015): Issue 1 (February 2015)
By: Haddadi Azam,  Somayeh Mikaili Ghezeljeh and  Shavandi Mahmoud  
Open Access
|Jan 2017

Figures & Tables

Figure 1

PCR analysis and restriction fragment length polymorphism analysis of PCR products of E. coli antibiotic resistance integrons. M: 100 bp ladder, Lane 1: positive control (491 bp), Lane 2: Class 1 integron (PCR product treated by HinfI, a 491 bp fragment), Lane 3: Class 1 integron (PCR product treated by RsaI, a 491 bp fragment), Lane 4: Class 2 integron (PCR product treated by HinfI, 300 and 191 bp fragments), Lane 5: Class 2 integron (PCR product treated by RsaI, 334 and 157 bp fragments).
PCR analysis and restriction fragment length polymorphism analysis of PCR products of E. coli antibiotic resistance integrons. M: 100 bp ladder, Lane 1: positive control (491 bp), Lane 2: Class 1 integron (PCR product treated by HinfI, a 491 bp fragment), Lane 3: Class 1 integron (PCR product treated by RsaI, a 491 bp fragment), Lane 4: Class 2 integron (PCR product treated by HinfI, 300 and 191 bp fragments), Lane 5: Class 2 integron (PCR product treated by RsaI, 334 and 157 bp fragments).

Association between antibiotic resistance and presence of integrons in E_ coli isolates

AntibioticResistant int-positive

int-positive = Integron positive

(Number of isolates)		Resistant int-negative

int-negative = Integron negative

(Number of isolates)		Total number of isolatesAssociation with integron
Nitrofurantoin1910<0.12
Levofloxacin102434<0.59
Amikacin31215<0.22
Chloramphenicol41519<0.87
Gentamycin62026<0.72
Nalidixic acid155267<0.44
Ciprofloxacin102737<0.23
Norfloxacin92635<0.23
Ofloxacin92534<0.32
Tetracycline155671<0.35
Amoxicillin206888<0.97
Co-trimoxazole194968<0.048
Imipenem82129<0.93
Cephalexin164965<0.37
Ceftriaxone113445<0.76
Cefotaxime113546<0.77
Cephalothin124254<0.74
Ceftazidime62733<0.34
Ceftizoxime52227<0.74
DOI: https://doi.org/10.5372/1905-7415.0901.367 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
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Language: English
Page range: 49 - 54
Published on: Jan 31, 2017
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year
Keywords:
ESBL,
Escherichia coli,
integron,
multidrug resistance,
PCR-RFLP
Related subjects:
Medicine,
Assistive professions, nursing,
Basic medical science,
Basic medical science, other,
Clinical medicine,
Clinical medicine, other

© 2017 Haddadi Azam, Somayeh Mikaili Ghezeljeh, Shavandi Mahmoud, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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