Have a personal or library account? Click to login

Optimal subculture methods for passaging and growing human epidermal keratinocytes

Open Access
|Feb 2017

References

  1. 1. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from signal cells. Cell. 1975; 6: 331-43.10.1016/S0092-8674(75)80001-8
  2. 2. Price RD, Das-Guptaf V, Frame JD, Navsaria HA. A study to evaluate primary dressings for the application of cultured keratinocytes. Br J Plast Surg. 2001; 54: 687-96.10.1054/bjps.2001.3712
  3. 3. Germain L, Rouabhia M, Guignard R, Carrier L, Bouvard V, Auger FA. Improvement of human keratinocyte isolation and culture using thermolysin. Burns. 1993; 19:99-104.10.1016/0305-4179(93)90028-7
  4. 4. Zhou KW, Luo QZ, Song HP, Huang LH, Zhao XF. Biological characteristics of human keratinocytes cultured in serum-free medium without feed layer. Chin J Burns. 2005; 21:438-41.
  5. 5. Li W, Wu JJ, Wu GX. Construction of human keratinocytes bank. Acta Academiae Medicinae Miltaris Tertiae. 2004; 26:987-9.
  6. 6. Liu SC, Karasek M. Isolation and growth of adult human epidermal keratinocytes in cell culture. J Invest Dermatol. 1978; 71:157-62.10.1111/1523-1747.ep12546943
  7. 7. Barker CL, Clothier RH. Human keratinocyte cultures as model of cutaneous esterase activity. Toxicol in Vitro. 1997; 11:637-40.10.1016/S0887-2333(97)00037-4
  8. 8. Ouyang AL, Zhou Y, Hua P, Tan WS. Effect of trypsin on the rat keratinocyte separation and subculture. Sheng Wu Gong Cheng Xue Bao. 2002; 18:59-62.
  9. 9. Kitano Y, Okada N. Separation of the epidermal sheet by dispase. Br J Dermatol. 1983; 108:555-60.10.1111/j.1365-2133.1983.tb01056.x6342649
  10. 10. Stenn KS, Link R, Moellmann G, et al. Dispase, a neutral protease from Bacillus polymyxa, is a powerful fibronectinase and type IV collagenase. J Invest Dermatol. 1989; 93:287-90.10.1111/1523-1747.ep122775932546994
DOI: https://doi.org/10.5372/1905-7415.0505.084 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
Language: English
Page range: 635 - 641
Published on: Feb 4, 2017
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year

© 2017 Yuanyuan Wang, Caimao Zhang, Tao Yang, Guihong Yang, Shuqian Tang, Jinjin Wu, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.