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Dutch Parelsnoer Institute-Cerebrovascular Accident (CVA) Study: A Large Multicenter Clinical Biobank with Standardized Collection and Storage Cover

Dutch Parelsnoer Institute-Cerebrovascular Accident (CVA) Study: A Large Multicenter Clinical Biobank with Standardized Collection and Storage

Open Access
|Jul 2018

Figures & Tables

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Figure 1

Organogram of the Parelsnoer Institute.

Table 1

Summary of procedures on collection, processing and storage of samples defined in the biobanking protocol of PSI.

SampleVolume/numberProcessingTimeAliquotingStorageAdditional information
Serum10 ml clotted blood2000 × g at room temperature or 4°C for 10 minutesWithin 2–4 hours≥5 × 0.5 ml–80°CSOP deviations*
EDTA plasma10 ml2000 × g at room temperature or 4°C for 10 minutesWithin 2–4 hours≥5 × 0.5 ml–80°CSOP deviations*
DNA (blood)10 ml EDTA bloodWhole blood or cell pellet, to UMC specificationsWithin 4 weeks (4°C) or 3 months (<–20°C)≥2 stock aliquots4°C or lowerOD ratio 260/280, concentration in μg/ml*

[i] * See the ‘Quality assurance measures’ section for detailed additional information.

DOI: https://doi.org/10.5334/ojb.41 | Journal eISSN: 2056-5542
Language: English
Published on: Jul 19, 2018
Published by: Ubiquity Press
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2018 H. J. A. van Os, Y. M. Ruigrok, J. Manniën, E. J. van Dijk, P. J. Koudstaal, G. J. Luijckx, P. J. Nederkoorn, R. J. van Oostenbrugge, M. C. Visser, L. J. Kappelle, A. Algra, M. J. H. Wermer, published by Ubiquity Press
This work is licensed under the Creative Commons Attribution 3.0 License.