The Quebec Respiratory Health Network Biobank

Figures & Tables

Repository sites of the Quebec Respiratory Health Network Biobank.

Site	Recruitment	Years active	Current researchers in charge
Institut universitaire de cardiologie et de pneumologie de Québec (IUCPQ)	active	1998–ongoing	François Maltais Philippe Joubert
Centre de recherche du Centre hospitalier de l’Université de Montréal (CRCHUM)	active	2011–ongoing	Emmanuelle Brochiero
Meakins-Christie Laboratories	active	1998–ongoing	Simon Rousseau Anne-Marie Lauzon
Université du Québec à Chicoutimi (UQAC)	active	2005–ongoing	Catherine Laprise
Centre hospitalier universitaire de Sherbrooke (CHUS)	inactive	1998–2006	NA
Centre hospitalier universitaire Sainte-Justine (CHUSJ)	development	2017–ongoing	Sze Man Tse

Stabilization, preservation and storage of biological samples.

Type of samples	Stabilization/preservation	Type of long-term preservation	Storage temperature
Serum	Tubes are plain VACUTAINER® containing no anticoagulant	Cryotube aliquots in ultra-low temperature freezers	–80°C
Plasma	Tubes with spray-coated K₂EDTA	Cryotube aliquots in ultra-low temperature freezers	–80°C
Buffy coat	Tubes with spray-coated K₂EDTA	Cryotube aliquots in ultra-low temperature freezers	–80°C
Tissue: Bronchial biopsies	10% neutral buffered formalin 4% formaldehyde solution	Paraffin Embedded	Room temperature
Muscle biopsies Nasal polyp Nasal mucosa	Tissue microarrays (TMA)
Non-tumor and Tumor lung Parenchyma	OCT: Embedding medium for frozen tissue specimens	Ultra-low temperature freezers	–80°C
Mucus Uvula	Snap frozen in liquid nitrogen	Ultra-low temperature freezers	–80°C
Tissue from lung transplant Trachea	Glycol Methacrylate (GMA) embedding medium	Freezers	–20°C
Induced sputum supernatants	With or without antiprotease	Cryotube aliquots in ultra-low temperature freezers	–80°C
Bronchoalveolar lavages supernatants	With or without antiprotease	Cryotube aliquots in ultra-low temperature freezers	–80°C
DNA	DNA is eluted in Buffer AE, Qiagen	Cryotube aliquots in ultra-low temperature freezers	–80°C
RNA	RNA is eluted in RNase-free water	Cryotube aliquots in ultra-low temperature freezers	–80°C
Lung primary cell cultures	DMSO	Liquid nitrogen tanks	–150°C –196°C

Clinical data collected by respiratory condition.

	Asthma	Lung cancer	COPD	Cystic fibrosis	Pulmonary hypertension	Interstitial diseases	ARDS
Subject biobank ID #
Gender
Ethnicity
Age at time of visit
Height
Weight
BMI
Death
Visit type
Date of visit
Study name (if applicable)
Version of consent form
Smoking history
Type of subject (tailored to each specific condition)
Allergy (yes or no and details on respiratory allergens)
Age of onset
Year of diagnosis
Other diseases
Pulmonary function testing
Blood tests (tailored to each specific condition)
Medical tests (tailored to each specific condition)
Medication
Date of surgery
Investigation
Pathology
Postoperative follow-up

[i] Grey shaded areas indicate that data are available.

The number of subjects and samples by medical condition.

	# subjects	# samples
Asthma	2540	17701
Lung cancer	3712	29165
COPD	510	6715
Cystic fibrosis	335	409
Lung transplant	34	26
Sleep apnea	50	255
Pulmonary arterial hypertension	483	2237
Interstitial lung diseases	244	545
Total	7908	57053

Sabrina Biardel	Coordinator of the Quebec RHN Biobank.
Christine Racine	Biobank manager at the IUCPQ.
Yvan Fortier	Database manager and Éric Rousseau is responsible for the database at the Laboratoire de Télématique Biomédicale (LTB) – CHUS.
Andrea Karen Mogas	Biobank manager at RI-MUHC/Meakins-Christie Laboratories.
Émilie Maillé	Biobank manager at the CRCHUM.
Emmanuelle Brochiero	Researcher in charge at the CRCHUM.
Anne-Marie Lauzon	Researcher in charge at the RI-MUHC/Meakins-Christie Laboratories.
François Maltais and Philippe Joubert	Researchers in charge at the IUCPQ.
Sze Man Tse	Researcher in charge at the CHU Sainte-Justine.
Catherine Laprise	Researcher in charge at UQAC.
Élyse Bissonnette	Director of the Quebec RHN and oversees the activities of the biobank.
Michel Laviolette	Founder and past Director of the Quebec RHN Biobank.
Yohan Bossé	Previous Director of the Quebec RHN Biobank and largely contributed to this manuscript.
Simon Rousseau	Director of the Quebec RHN Biobank.