Figure 1
Bimetra, Clinical Research Center Ghent.
Figure 2
Bimetra Biobank governance structure.
Table 1
Generic processing protocol data.
| Type | sample type | primary collection tube | Processing | Freezing | Aliquots | Storage (main storage) |
|---|---|---|---|---|---|---|
| liquid sample | serum | serum separation tube with clot activator | 1500 g at RT, 10 min | slow rate freezing, ULT | 3 to 6 | –80°C |
| liquid sample | plasma | Potassium EDTA tube | 815 g, 4°C, 10 min and 2500 g, 4°C, 10 min | slow rate freezing, ULT | 3 to 6 | –80°C |
| liquid sample | plasma low platelet | Potassium EDTA tube | 3200 g at RT, 12 min and 2000 g, 4°C, 10 min | slow rate freezing, ULT | 3 to 6 | –80°C |
| liquid sample | plasma and RBC rest | Potassium EDTA tube | 815 g, 4°C, 10 min and 2500 g, 4°C, 10 min | slow rate freezing, ULT | 3 to 6 | –80°C |
| liquid sample | buffy coat, viable PBMC | Potassium EDTA tube | buffy coat separation with Lymphoprep | controlled rate freezing, ULT | up to 20 | isothermal freezer |
| liquid sample | cell lines | NA | NA | controlled rate freezing, device | 1 to 10 | isothermal freezer |
| liquid sample | urine | 30 ml PP tube | aliquoting | slow rate freezing, ULT | 1 | –80°C |
| liquid sample | semen | straws | aliquoting | controlled rate freezing, device | multiple | isothermal freezer |
| liquid sample | reproductive material | cryovials and straws | aliquoting | controlled rate freezing, device | multiple | isothermal freezer |
| solid sample | stool | 30 ml PP tube and 1.5 ml PP tube | weighing and aliquoting | slow rate freezing, ULT | 1 to 10 | –80°C |
| solid sample | frozen tissue, tumor | NA | macroscopical dissection and freezing | snapfreezing isopenthane | 1 to 5 | –80°C |
| solid sample | frozen tissue, normal | NA | macroscopical dissection and freezing | snapfreezing isopenthane | 1 to 5 | –80°C |
| solid sample | frozen tissue, other | NA | macroscopical dissection and freezing | snapfreezing isopenthane | 1 to 5 | –80°C |
| solid sample | FFPE tissue | NA | formalin fixation and parafin embedding | NA | 1 to 5 | RT |
| solid sample | reproductive material | cryovials | macroscopical dissection and freezing | controlled rate freezing, device | multiple | isothermal freezer |
Figure 3
Overview of the data-structure of the Flemish Biobank Backbone.
Figure 4
Bimetra Biobank: overview of sample storage and sample exchange (up to September 2017).
Figure 5
Bimetra Biobank: overview of sample storage and sample exchange (up to September 2017).