Figure 1
Workflow overview of the Biobank of Psychiatric Diseases Mannheim – BioPsy. a) Pre-analysis of BioPsy workflow. b) Processing and analysis of BioPsy workflow (Abbreviations: EDTA = Ethylenediaminetetraacetic acid; CAT = Clot Activator Tube; CPTTM = Cell Preparation Tube; PBMC = peripheral blood mononuclear cell; DNA = Deoxyribonucleic acid; RNA = Ribonucleic acid; r.t. = room temperature).
Table 1
Overview of type, processing, aliquot number, volume and storage temperature of collected biospecimen.
| Biospecimen Type | Biospecimen Processing | Number of Aliquots | Size (mm) / Volume (ml) | Storage Temperature |
|---|---|---|---|---|
| Peripheral Blood | Whole blood (EDTA) | 1–3 | 9 ml | –20°C short term / –80°C long term |
| PAXgene®blood | 1 | 9 ml | –80°C | |
| Plasma | Up to 7 | 0.5 ml | –80°C | |
| Serum | Up to 7 | 0.5 ml | –80°C | |
| Archive | 1 | 20 × 60 mm | 18–25°C | |
| Saliva | OrageneTM | 1 | 4 ml | –80°C |
| Tissue | Placenta | 1 | 50 ml | –80°C |
| Hair | Hair | 1 | 40 × 120 mm | 18–25°C |
| Body fluids | Amniotic fluid | 5 | 0.5 ml | –80°C |
| Amniotic cell culture | 1 | 0.3 ml | –80°C | |
| Urine | 5 | 1 ml | –20°C | |
| Processed | DNA | 2 | 1 ml | –20°C |
| RNA | 5 | 0.03 ml | –80°C | |
| PBMC | 1 | 1 ml | –80°C |