Pluvianus: Interactive GUI for Exploration and Quality Control of CaImAn Calcium Imaging Analysis Results

Pluvianus is a stand-alone graphical user interface designed for the visual exploration and quality control of calcium imaging results produced by CaImAn’s CNMF and OnACID algorithms. It provides synchronized spatial and temporal views, intuitive visual comparison tools, and interactive component acceptance, while imposing no constraints on the underlying computational workflow or data organization, enhancing integration flexibility. The software was tested on CaImAn demo datasets as well as on in-house measured cortical calcium activity data, demonstrating that the GUI enables verification of CaImAn outputs and manual curation of cell detection. Publicly distributed on GitHub under the MIT license, Pluvianus supports seamless adoption and community-driven development, making it broadly usable for neuroscientists working with calcium imaging data across diverse experimental paradigms.