VScope — Data Acquisition and Analysis for Voltage-sensitive Dye Imaging Using Multiple Cameras and Electrophysiology

Daniel A. Wagenaar

doi:10.5334/jors.176

Abstract

VScope is a software package for the acquisition and analysis of data from multiple cameras as well as electrophysiology. Its main intended purpose is to record fluorescent traces from neurons loaded with voltage-sensitive dyes along with associated electrophysiology. VScope can record simultaneously from any number of cameras and frame rates can be set independently for each camera. VScope can also record synchronized electrophysiology traces and digital channels. VScope is mainly intended to acquire fixed-duration trials, but it can also acquire electrophysiology continuously. A variety of electrical stimulation protocols can be created through an easy-to-use GUI.

Funding statement: VScope was developed in conjunction with research funded by the Broad Foundations (Broad Fellowship program at Caltech); the Burroughs Wellcome Fund (Career Award at the Scientific Interface #1007977) and the National Institutes of Health (R01-NS-094403).

References

Tomina Y Wagenaar D A Pan-neuronal recording in the leech nervous system using dual-sided voltage sensitive dye imaging Society for Neuroscience, 46th annual meeting 2016 San Diego CA abstract no 337.10
Search in Google Scholar Back to article
Baca S M Marin-Burgin A Wagenaar D A Kristan W B Jr Widespread inhibition proportional to excitation controls the gain of a leech behavioral circuit Neuron 2008 57 2 276 289 10.1016/j.neuron.2007.11.028 PMID 18215624
Open DOI Search in Google Scholar Back to article
Briggman K L Abarbanel H D I Kristan W B Optical imaging of neuronal populations during decision-making Science 2005 307 5711 896 901 10.1126/science.1103736 PMID 15705844
Open DOI Search in Google Scholar Back to article
Cacciatore T W Brodfuehrer P D Gonzalez J E Jiang T Adams S R Tsien R Y Kristan W B Kleinfeld D Identification of neural circuits by imaging coherent electrical activity with FRET-based dyes Neuron 1999 23 3 449 459 10.1016/S0896-6273(00)80799-0
Open DOI Search in Google Scholar Back to article
Eaton J W Bateman D Hauberg S Wehbring R The GNU Octave 4.0 Reference Manual: Free Your Numbers 2015 URL: http://www.gnu.org/software/octave/doc/interpreter retrieved May 9, 2017
Search in Google Scholar Back to article
Muthusamy A Lehmkuhl A M Wagenaar D A S-cell responses to visual and mechanical water waves in the leech Hirudo verbana Society for Neuroscience, 46th annual meeting 2016 San Diego CA abstract no 337.12
Search in Google Scholar Back to article
Baljon P L Wagenaar D A Responses to conflicting stimuli in a simple stimulus-response pathway J Neurosci 2015 35 6 2398 2406 10.1523/JNEUROSCI.3823-14.2015 PMID 25673834
Open DOI Search in Google Scholar Back to article

VScope — Data Acquisition and Analysis for Voltage-sensitive Dye Imaging Using Multiple Cameras and Electrophysiology

Abstract

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