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Hands-on Hypoxia: Engaging High School Educators in the science behind Marine Microbial Dynamics in Hypoxic Coastal Areas Through Field and Classroom Experiences Cover

Hands-on Hypoxia: Engaging High School Educators in the science behind Marine Microbial Dynamics in Hypoxic Coastal Areas Through Field and Classroom Experiences

Open Access
|Oct 2023

Figures & Tables

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Figure 1

Photosynthesis and respiration equation.

Table 1

Agenda for the workshop, time spent on each activity, and educational standards for Next Generation Science Standards (NGSS), Ocean Literacy Principles (OLP), California Common Core Standards: Reading Standards for Literacy in Science and Technical Subjects 6–12 (CCCS-R), California Common Core Standards: Writing Standards for Literacy in History/Social Studies, Science, and Technical Subjects 6–12 (CCCS-W), and A Framework for K-12 Science Education (Framework).

LOCATIONAGENDAEDUCATIONAL STANDARDS
Day oneUSC’s University Park CampusIntroduction to marine microbial ecology lecture (2 h), Thrash Lab tour (2 h), Lesson 1: Exploring the Diversity of Marine Microbes (2 h)NGSS HS-LS2-6 & HS-LS2-2; OLP 2, 3, 5; CCCS-R 3 & 4; CCCS-W 2 & 7; Framework PS3.D, LS4.C, LS2.B, ESS2.D, ESS3.D
Day twoWrigley Institute for Environmental Studies (WIES) on Catalina Island, CaliforniaLesson 1: Exploring the Diversity of Marine Microbes (3 h), research lab tours (3 h)NGSS HS-LS2-6 & HS-LS2-2; OLP 2, 3, 5; CCCS-R 3 & 4; CCCS-W 2 & 7; Framework PS3.D, LS4.C, LS2.B, ESS2.D, ESS3.D
Day threeUSC’s University Park CampusLesson 2: Bacterial Ecology and Physiology (2 h)NGSS HS-LS2-2 & HS-LS2-6; OLP 2, 3, 5; CCCS-R 3 & 4; CCCS-W 2 & 7; Framework LS2.B, ESS2.D, ESS3.D
Lesson 3: Circulation of Nutrients (2 h)NGSS HS-ESS3-5 & HS-ESS2-2; OLP 1, 2, 3; CCCS-R 3 & 4; CCCS-W 2 & 7; Framework LS2.B, ESS2.D, ESS3.D
Lesson 4: Deoxygenation and Measuring Dissolved Oxygen (2 h)NGSS HS-ESS3-5 & HS-ESS2-2; OLP 1, 3, 4, 5; CCCS-R 3 & 4; CCCS-W 2 & 7; Framework LS2.B, ESS2.D, ESS3.D
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Figure 2

Educators look at a culture flask full of microbes (left) and learn about flow cytometry and cell sorting (right). Photos by Dr. Dieuwertje Kast.

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Figure 3

Teachers participate in the great plankton race activity from Lesson 1. A few examples of the phytoplankton teachers created are displayed. Photos by Dr. Dieuwertje Kast.

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Figure 4

Educators take turns collecting water in a Niskin bottle to measure dissolved oxygen with an optode sensor (top left), measuring turbidity with a Secchi disk (top right), collecting phyto- and zooplankton in net tows (bottom left), and measuring salinity with a refractometer (bottom right). Photos by Dr. Dieuwertje Kast.

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Figure 5

Educators use microscopes at WIES to look at the phyto- and zooplankton samples they collected (left). One microscope was connected to a screen to project the view under the scope (right). Photos by Dr. Dieuwertje Kast and Kyla Kelly.

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Figure 6

A visual example of serial dilutions was made using food coloring and distilled water (top). In Lesson 2, teachers performed serial dilutions with bacterial cultures and plated them on agar (middle). Twenty-four hours later, the number of bacterial colonies on each plate were counted and quantified (bottom). Photos by Dr. Dieuwertje Kast and Kyla Kelly.

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Figure 7

Teachers measure dissolved oxygen content of water samples with different salinities and temperatures in Lesson 4. Photos by Dr. Dieuwertje Kast.

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Figure 8

In Lesson 3, educators poured water of different densities down a gently sloping surface (top left). The different water bodies (indicated by different colors) separated into layers based on density (top right). To demonstrate the separation of bodies of water with different densities, cups with cold, saline water (blue) and hot, fresh water (red) were placed in a shallow container of water (bottom left). Holes were poked in the cups to allow water to slowly trickle out. The water masses separated in the container, based on density (bottom right). Photos by Dr. Dieuwertje Kast.

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Figure 9

Educator survey responses from the pre-knowledge assessment. Educators were asked to rank the following statements on the provided scale. Of the 8 teachers that attended the workshop, 7 responded to the surveys.

DOI: https://doi.org/10.5334/cjme.86 | Journal eISSN: 2632-850X
Language: English
Submitted on: Sep 7, 2022
Accepted on: Aug 2, 2023
Published on: Oct 3, 2023
Published by: Ubiquity Press
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2023 Kyla J. Kelly, Dieuwertje J. Kast, Cara A. Schiksnis, J. Cameron Thrash, published by Ubiquity Press
This work is licensed under the Creative Commons Attribution 4.0 License.