Figure 1
Geographic location of the municipality of Moniquirá (area highlighted in red on the main map), department of Boyacá.
Table 1
Resistance profile to lambda-cyhalothrin of Aedes aegypti mosquitoes from Moniquirá, Boyacá, Colombia.
| Doses (ppm) | N° larvae | Death Larvae/total | Mortality (%) |
|---|---|---|---|
| 0.00188 | 20 | 35/180 | 19.44 |
| 0.00375 | 20 | 73/180 | 40.55 |
| 0.00750 | 20 | 143/180 | 79.44 |
| 0.01500 | 20 | 168/180 | 93.33 |
| 0.03000 | 20 | 177/180 | 98.33 |
| 0.06000 | 20 | 179/180 | 99.44 |
[i] LD50: 0,004124 Lim(0,002–0,006); LD90: 0,013 Lim(0,013–0,016).
Figure 2
Susceptibility of adults of Moniquirá A. aegypti strain to malathion insecticide. On the left “y” axis the adult mortality for malathion is shown at a diagnostic dose and time of 50 μg/mL and 30 minutes, respectively.
Table 2
Allele and genotype frequencies and associated P values for chi-squared tests for deviation from Hardy-Weinberg equilibrium of sodium channel mutations in Aedes aegypti from Moniquirá, Boyacá; Colombia.
| Mutation | n | Allelic frequency | Genotype | Hardy-Weinberg equilibrium analysis | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Wild | Mutated | Wild Homozygous | Heterozygous | Mutated homozygous | Ho | He | X2 | P | ||
| 419 | 36 | 0.61 | 0.39 | 0.33 | 0.56 | 0.11 | 0.56 | 0.48 | 0.0285 | 0.48 |
| 1016 | 34 | 0.60 | 0.40 | 0.32 | 0.56 | 0.12 | 0.56 | 0.48 | 0.0279 | 0.41 |
| 1558 | 36 | 0.06 | 0.94 | 0.00 | 0.11 | 0.89 | 0.11 | 0.10 | 0.0035 | 1.00 |
Figure 3
Identification of arboviruses in A. aegypti from Moniquirá. A. 2.5% agarose gel electrophoresis of the RT-PCR products visualized using the intercalating ethidium bromide. Positive controls D1: DENV-1 (169 bp), D2: DENV-2 (362 bp), D3: DENV-3 (265 bp), D4: DENV-4 (426 bp), Z: ZIKV (192 bp), C: CHIKV (294 bp). Line 1–9: Pools of A. aegypti. M: 100 bp molecular weight marker. B. Confirmation of infection with dengue virus DENV-1. Agarose gel electrophoresis at 2.5% of the species-specific RT-PCR products visualized with the intercalator ethidium bromide. C–: Negative control, 1–3, 5, and 8: pools of A. aegypti. D1: positive control DENV-1 (169 bp). M: 100 bp molecular weight marker.