
Figure 1
Representative bright field image from a microscope in Belize showing obstructed field of view due to accumulation of debris on objective, eyepieces, and internal components. Debris is likely comprised of fungal growth and dust, containing both organic and inorganic matter.

Figure 2
Humidity control system containing microscope.
Table 1
Material list for humidity control chamber. Similar locally sourced materials may be used as substitutes.
| Materials | Cost |
|---|---|
| 500 mL reusable silica beads | ~$2.00 |
| Garbage bag (~50 gallon) | ~$0.10 |
| 500 g sand, packing tape, mason jar | <$0.25 |
| Punctured 26” bicycle tube | $0.00 |
| Glass jar for silica beads | $0.00 |

Figure 3
Humidity control chamber fabrication instructions.

Figure 4
Relative humidity in and out of chamber located at the University of Belize (A) and the Belize Vector and Ecology Center (B). Data collected every 30 minutes with DHT11 sensor with an Arduino UNO. An autoregressive model demonstrates external and internal relative humidity are different; p < 0.001.
