Anther Culture Potential of Indica Rice Varieties, Kurulu Thuda and BG 250
By: TD Silva and WJ Ratnayake
Open Access
|Feb 2011Abstract
The anther culture ability of two local indica rice varieties were evaluated by anther plating on N6 and SK-I callus induction media. The traditional rice variety, Kurulu Thuda displayed good anther culture potential with a callus induction frequency of 17.2%. Anthers most suitable for culture were those bearing bi-nucleate pollen. Anther response was relatively poor in the new improved variety, Bg 250 (1.4%). Callus induction was nearly three times higher on N6 medium than on SK-I medium in var. Kurulu Thuda. Shoot regeneration was achieved in var. Kurulu Thuda on SK-II medium, but only from callus induced on N6 medium. From a total of 115 calli transferred from N6 to regeneration medium shoot development occurred in 7 calli, thus limiting the overall anther culture efficiency. Of the calli with morphogenetic competence, 5 gave rise to albino shoots and 2 produced green shoots. After root development the green plantlets were potted in soil. Plant regeneration could not be observed from callus induced in var. Bg 250.
Key words: Anther culture; Callus induction; Indica rice
DOI: 10.4038/tare.v12i2.2789
Tropical Agricultural Research & Extension 12(2): 2009 pp.53-56
Key words: Anther culture; Callus induction; Indica rice
DOI: 10.4038/tare.v12i2.2789
Tropical Agricultural Research & Extension 12(2): 2009 pp.53-56
DOI: https://doi.org/10.4038/tare.v12i2.2789 | Journal eISSN: 1391-3646
Language: English
Page range: 53 - 56
Published on: Feb 1, 2011
Published by: Faculty of Agriculture, University of Ruhuna
In partnership with: Paradigm Publishing Services
Keywords:
© 2011 TD Silva, WJ Ratnayake, published by Faculty of Agriculture, University of Ruhuna
This work is licensed under the Creative Commons License.