Preliminary study on cytotoxic compounds from the seeds of Nigella sativa L (Black cumin)

Isolation and characterization of cytotoxic compounds from the seeds of Nigella sativa L is presented. The oil present in seeds was isolated by steam distillation followed by extraction with Diethyl ether. The cytotoxic activity of the oil was investigated by carrying out bioassays with bacteria (Erwinia carotovora) and MosquitoL larvae. The seed oils of N. sativa L showed significant cytotoxic effect towards the Erwinia carotovora and mosquito larvae. The cytotoxic compounds were separated by column chromatography and characterized by chemical and spectroscopic methods. These cytotoxic compounds were identified as terpenoids containing carbonyl and hydroxyl functionalities.