Comparison of Sperm Count between the Established Makler Chamber and the Newer Rohem Chamber
Abstract
Seminal fluid analysis is used to determine sperm concentration and assess male fertility. This study was designed to compare the sperm-counting efficiency of the Makler and Rohem chambers.
A cross-sectional comparative study was conducted at Teaching Hospital Jaffna from September 2022 to April 2023 among 100 men. Semen samples were analysed using both chambers, and the Bland-Altman plots test was performed to evaluate the agreement between the Rohem chamber and the Makler chamber.
The mean + standard deviation (Makler-56.38 + 50.01 & Rohem-117.79 + 96.04) showed great differences between the both chambers with statistical significance (p<0.001). Additionally, the Bland-Altman graph revealed that the majority of the readings were outside the upper and lower limits in the 95% confidence interval, showing a lower chance of clinical agreement between both chambers.
The study findings suggest that using the Rohem chamber instead of the Makler chamber to determine the sperm concentration is suboptimal.
A cross-sectional comparative study was conducted at Teaching Hospital Jaffna from September 2022 to April 2023 among 100 men. Semen samples were analysed using both chambers, and the Bland-Altman plots test was performed to evaluate the agreement between the Rohem chamber and the Makler chamber.
The mean + standard deviation (Makler-56.38 + 50.01 & Rohem-117.79 + 96.04) showed great differences between the both chambers with statistical significance (p<0.001). Additionally, the Bland-Altman graph revealed that the majority of the readings were outside the upper and lower limits in the 95% confidence interval, showing a lower chance of clinical agreement between both chambers.
The study findings suggest that using the Rohem chamber instead of the Makler chamber to determine the sperm concentration is suboptimal.
DOI: https://doi.org/10.4038/jmj.v37i1.231 | Journal eISSN: 2651-0200
Language: English
Page range: 10 - 12
Published on: Jul 31, 2025
Published by: The Jaffna Medical Association
In partnership with: Paradigm Publishing Services
© 2025 Raguraman Sivalingarajah, Balasigam Balagobi, S. Abirame, R. Nirojee, Shereone A. Vethanayakam, Thayalini Surkirthan, Rajasingam S. Jeyendran, published by The Jaffna Medical Association
This work is licensed under the Creative Commons Attribution 4.0 License.