RNA extraction from urine sediment: A cost-effective protocol for gene expression analysis in renal pathology
By: S. Saseevan, S. Rajapakse and D. N. Magana-Arachchi
Abstract
Urine is an appropriate choice of specimen to study the biomarkers for metabolic and renal disorders because it is readily available with less harm to the patients. However, RNA extraction from voided urine is challenging due to the presence of RNases and cell scarcity. This study aims to optimize a protocol for RNA extraction from urine samples for gene expression studies in renal pathology. Hundred and two urine samples were collected from both healthy controls (HC) (
DOI: https://doi.org/10.4038/cjs.v51i5.8080 | Journal eISSN: 2513-230X
Language: English
Page range: 531 - 539
Published on: Dec 31, 2022
Published by: Faculty of Science, University of Peradeniya, Sri Lanka
In partnership with: Paradigm Publishing Services
© 2022 S. Saseevan, S. Rajapakse, D. N. Magana-Arachchi, published by Faculty of Science, University of Peradeniya, Sri Lanka
This work is licensed under the Creative Commons Attribution 4.0 License.