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Resistance priming in improving strength: the effects of unilateral isometric strength activation on cross-activation of bilateral quadriceps and hamstring strength at 24 and 48 hours Cover

Resistance priming in improving strength: the effects of unilateral isometric strength activation on cross-activation of bilateral quadriceps and hamstring strength at 24 and 48 hours

Open Access
|Dec 2025

Abstract

Purpose: This study aims to investigate whether unilateral low-volume, high-intensity isometric strength activation (ISA) can enhance jump performance and bilateral isokinetic flexion and extension strength within 24 and 48 hours post-intervention.

Methods: A total of 68 participants (40 males and 28 females) were included, all free from muscle, ligament or skeletal disorders that could affect physical performance, and none had undergone lower limb surgery due to injury in the past year. Participants were randomly assigned to either the experimental group or the control group using a balanced randomization scheme. Athletic performance was assessed using unloaded countermovement jump (CMJ), unloaded squat jump (SJ), and isokinetic knee flexion and extension strength tests. The experimental group received an isometric activation protocol, while the control group maintained their regular exercise routines.

Results: The isometric activation protocol led to varying degrees of improvement across genders in the experimental group. Among male participants, there were significant increases in CMJ performance 24 hours post-activation, with flight time ((FT): +5%) and jump height ((JH): +9%) both showing statistical significance ( p < 0.05). SJ performance also improved significantly, with FT ( p < 0.01, ES = 1.101) and JH ( p < 0.01, ES = 1.335) demonstrating large effect sizes. Furthermore, SJ performance remained significantly elevated 48 hours post-intervention compared to baseline ( p < 0.05, JH: ES = 0.829). For female participants, SJ performance showed significant improvement 24 hours after activation ( p < 0.05, FT: ES = 1.847; JH: ES = 1.789), although no other significant changes were observed. Regarding knee flexion and extension strength, at an angular velocity of 60°/s, the male group exhibited significantly greater strength at 48 hours post-intervention compared to 24 hours ( p < 0.05, ES = 1.791). In the female group, bilateral knee strength significantly improved at both 24 and 48 hours post-intervention ( p < 0.05, ES = 0.152).

Conclusions: ISA interventions can enhance knee joint strength in both male and female participants within 24 and 48 hours post-intervention, and also induce a cross-activation effect. Therefore, when coaches aim to improve athletes’ performance in subsequent training sessions or competition days, ISA can be considered as an effective method to activate lower limb strength.

DOI: https://doi.org/10.37190/abb/208438 | Journal eISSN: 2450-6303 | Journal ISSN: 1509-409X
Language: English
Page range: 39 - 48
Submitted on: Jun 10, 2025
Accepted on: Jul 20, 2025
Published on: Dec 11, 2025
Published by: Wroclaw University of Science and Technology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Longzhen Wang, Xiangrong Cheng, Jingyuan Wang, published by Wroclaw University of Science and Technology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.