Association between single nucleotide polymorphisms and viral load in congenital cytomegalovirus infection

Jedlińska-Pijanowska, Dominika; Kasztelewicz, Beata; Dobrzańska, Anna; Dzierżanowska-Fangrat, Katarzyna; Jaworski, Maciej; Czech-Kowalska, Justyna

doi:10.34763/jmotherandchild.20202404.d-20-00014

Figures & Tables

HCMV VL in urine depending on genotypes of IL12B rs3212227 polymorphism. Bars in the box show the first and the third quartiles. Whisker charts represent the minimum to maximum values. Small box represents median value. p-value below 0.05 is statistically significant. Kruskal-Wallis ANOVA test was used. HCMV: human cytomegalovirus; VL: viral load.

Maternal and neonatal demographic data and general characteristics of cCMV-infected infants, with subgroups of symptomatic and asymptomatic infants_

Characteristics	cCMV-infected infants (n = 92)	Symptomatic infants (n = 73)	Asymptomatic infants (n = 19)	p-value p-value for comparison between symptomatic and asymptomatic infants.
Maternal age at delivery, (years)	28 (25–31)	29 (25–31)	28 (25–31)	NS
Primipara, n (%)	42 (45.65)	33 (45.21)	9 (47.37)	NS
Preterm delivery (<37Hbd), n (%)	23 (25.00)	19 (26.03)	4 (21.05)	NS
Caesarean section, n (%)	49 (54.44)	45 (61.64)	4 (21.05)	0.006
Prenatal HCMV screening, n (%)	37 (40.22)	27 (36.99)	10 (52.63)	NS
Birth weight, (g)	(21702755 –3200)	(21502685 –3140)	(26803050 –3630)	0.028
Gestational age, (weeks)	38 (37–39)	38 (37–39)	39 (37–40)	NS
Male, n (%)	46 (50.00)	36 (49.32)	10 (52.63)	NS
Age at admission to NICU, Median (IQR), (days)	16 (9–23)	14 (9–21)	22 (20–35)	<0.001
Weight at admission to NICU, (g)	3040 (2440–3450)	2980 (2340–3320)	3600 (2930–4310)	<0.001
Age at first HCMV DNA detection in urine, (days)	9 (4–15)	8 (3–14)	17 (8–20)	0.002

Allele frequencies of examined SNPs in the study population of cCMV-infected neonates and subgroups of symptomatic and asymptomatic neonates

SNP,rs number SNP database (dbSNP) reference number (ID number). , alleles	Allele	cCMV-infected infants (n = 92)	Symptomatic infants (n = 73)	Asymptomatic infants (n = 19)	p-value p-value for comparison between frequencies of alleles and subgroups of symptomatic and asymptomatic CMV-infected newborn infants.

		Allele frequencies, n (%)
IL1B	G	121 (65.76)	97 (66.44)	24 (63.16)
rs16944					NS
G/A	A	63 (34.24)	49 (33.56)	14 (36.84)
IL12B	T	142 (77.17)	110 (75.34)	32 (84.21)
rs3212227					NS
G/T	G	42 (22.83)	36 (24.66)	6 (15.79)
IL28B	C	120 (65.22)	92 (63.01)	28 (73.68)
rs12979860					NS
C/T	T	64 (34.78)	54 (36.99)	10 (26.32)
CCL2	A	139 (75.54)	110 (75.34)	29 (76.32)
rs1024611					NS
A/G	G	45 (24.46)	36 (24.66)	9 (23.68)
DC-SIGN	G	107 (58.15)	87 (59.59)	20 (52.63)
rs735240					NS
A/G	A	77 (41.85)	59 (40.41)	18 (47.37)
TLR2	G	174 (94.57)	138 (94.52)	36 (94.74)
rs5743708					NS
A/G	A	10 (5.43)	8 (5.48)	2 (5.26)
TLR4	C	175 (95.11)	139 (95.21)	36 (94.74)
rs4986791					NS
C/T	T	9 (4.89)	7 (4.79)	2 (5.26)
TLR9	T	107 (58.15)	84 (57.53)	23 (60.53)
rs352140					NS
C/T	C	77 (41.85)	62 (42.47)	15 (39.47)

Allele frequencies of examined SNPs in the study population in comparison to known European and Global reference populations from the Reference SNP (rs) Report

SNP,rs number SNP database (dbSNP) reference number (ID number). , alleles	Allele	All study population (n = 233)	Uninfected control infants (n = 141)	cCMV-infected infants (n = 92)	p-value p-value for comparison between frequencies of alleles and subgroups of uninfected controls and CMV-infected newborn infants.	Reference population Reference allele frequency based on 1000Genomes study from the Reference SNP (rs) Report.
						European	Global
		Allele frequencies, n (%)				Allele frequencies, %
IL1B	G	320 (68.67)	199 (70.57)	121 (65.76)		65.01	49.06
rs16944					NS
G/A	A	146 (31.33)	83 (29.43)	63 (34.24)		34.99	50.94
IL12B	T	352(75.54)	210 (74.47)	142 (77.17)		77.73	64.10
rs3212227					NS
G/T	G	114 (24.46)	72 (25.53)	42 (22.83)		22.27	35.90
IL28B	C	309 (66.31)	189 (67.02)	120 (65.22)		69.09	64.42
rs12979860					NS
C/T	T	157 (33.69)	93 (32.98)	64 (34.78)		30.91	35.58
CCL2	A	344 (73.82)	205 (72.70)	139 (75.54)		68.39	63.64
rs1024611					NS
A/G	G	122 (26.18)	77(27.30)	45 (24.46)		31.61	36.36
DC-SIGN	G	278 (59.66)	171 (60.64)	107 (58.15)		56.76	64.70
rs735240					NS
A/G	A	188 (40.34)	111 (39.36)	77 (41.85)		43.24	35.30
TLR2	G	441 (94.64)	267 (94.68)	174 (94.57)		97.61	99.32
rs5743708					NS
A/G	A	25 (5.36)	15 (5.32)	10 (5.43)		2.39	0.68
TLR4	C	440 (94.42)	265 (93.97)	175 (95.11)		94.23	95.93
rs4986791					NS
C/T	T	26 (5.58)	17 (6.03)	9 (4.89)		5.77	4.07
TLR9	T	269 (57.73)	162 (57.45)	107 (58.15)		54.57	41.55
rs352140					NS
C/T	C	197 (42.27)	120 (42.55)	77 (41.85)		45.43	58.45

Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in asymptomatic newborn infants with cCMV infection

Asymptomatic newborn infants with cCMV infection (N = 19)
SNP, rs number SNP database (dbSNP) reference number (ID number). , alleles	Allele	n, (%)	HCMV DNA (copies/mL) in blood	p-value p-value – for comparison between alleles and viremia.	HCMV DNA (copies/mL) in urine	p-value p-value – for comparison between alleles and viruria.
IL1B	G	24 (63.16)	2.0 × 10³ (7.5 × 10²–3.3 × 10³) (n = 20)		4.9 ×10⁵ (1.4 × 10⁵–5.3 × 10⁶)
rs16944				NS		NS
G/A	A	14 (36.84)	1.8 × 10³ (9.9 × 10²–2.7 × 10⁴)		2.4 ×10⁶ (1.7 × 10⁵–6.1 × 10⁶)
IL12B	T	32 (84.21)	1.8 × 10³ (9.9 × 10²–1.5 × 10⁴) (n = 28)		6.9 ×10⁵ (1.4 × 10⁵–5.3 × 10⁶)
rs3212227				NS		NS
G/T	G	6 (15.79)	2.1 × 10³ (0.0–4.0 × 10³)		1.0 ×10⁶ (1.7 × 10⁵–2.0 × 10⁷)
IL28B	C	28 (73.68)	2.1 × 10³ (6.5 × 10²–2.7 × 10⁴) (n = 24)		1.3 ×10⁶ (9.1 × 10⁴–6.1 × 10⁶)
rs12979860				NS		NS
C/T	T	10 (26.32)	1.2 × 10³ (9.9 × 10²–2.7 × 10³)		2.9 ×10⁵ (2.5 × 10⁵–2.8 × 10⁶)
CCL2	A	29 (76.32)	1.8 × 10³ (9.9 × 10²–4.0 × 10³) (n = 26)		6.9 ×10⁵ (1.7 × 10⁵–6.1 × 10⁶)
rs1024611				NS		NS
A/G	G	9 (23.68)	2.4 × 10³ (9.5 × 10²–1.5 × 10⁴) (n = 8)		2.5 ×10⁵ (1.4 × 10⁵–3.2 × 10⁶)
DC-SIGN	G	20 (52.63)	1.2 × 10³ (1.5 × 10²–3.0 × 10³) (n = 16)		4.9 ×10⁵ (1.4 × 10⁵–7.5 × 10⁶)
rs735240				NS		NS
A/G	A	18 (47.37)	2.1 × 10³ (1.2 × 10³–3.2 × 10⁴)		1.3 ×10⁶ (1.7 × 10⁵–4.6 × 10⁶)
TLR2	G	36 (94.74)	2.0 × 10³ (6.5 × 10²–1.5 × 10⁴) (n = 32)		1.3 ×10⁶ (1.4 × 10⁵–6.1 × 10⁶)
rs5743708				NS		NS
A/G	A	2 (5.26)	1.4 × 10³ (1.2 × 10³–1.6 × 10³)		2.3 ×10⁵ (1.7 × 10⁵–2.9 × 10⁵)
TLR4	C	36 (94.74)	2.0 × 10³ (9.9 × 10²–1.5 × 10⁴) (n = 32)		6.9 ×10⁵ (1.5 × 10⁵–5.3 × 10⁶)
rs4986791				NS		NS
C/T	T	2 (5.26)	2.0 × 10³ (0–4.0 × 10³)		1.0 ×10⁷ (0–2.0 × 10⁷)
TLR9	T	23 (60.53)	1.8 × 10³ (1.0 × 10³–4.0 × 10³) (n = 20)		2.9 ×10⁵ (1.4 × 10⁵–6.1 × 10⁶)
rs352140				NS		NS
C/T	C	15 (39.47)	2.0 × 10³ (3.1 × 10²–2.7 × 10⁴) (n = 14)		2.8 ×10⁶ (4.0 × 10⁴–6.1 × 10⁶)

Association between genotypes of examined polymorphisms (SNPs) and viremia and viruria in newborn infants with cCMV infection_

SNP, rs number SNP database (dbSNP) reference number (ID number). , alleles	Genotype	n = 92, n (%)	HCMV DNA (copies/mL) in blood	p-value p-value – for comparison between genotypes and viremia.	HCMV DNA (copies/mL) in urine	p-value p-value – for comparison between genotypes and viruria.
IL1B	A/A	7 (7.6)	6.3 × 10³ (3.8 × 10³–2.7 × 10⁴)		6.5 × 10⁶ (2.8 × 10⁶–1.0 × 10⁷)
rs16944	A/G	49 (53.3)	8.6 × 10³ (1.9 × 10³–5.8 × 10⁴) (n = 48)	NS	7.8 × 10⁶ (5.1 × 10⁵–1.0 × 10⁷) (n = 48)	NS
G/A	G/G	36 (39.1)	1.5 × 10⁴ (2.0 × 10³–2.0 × 10⁵) (n = 34)		1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷) (n = 35)
IL12B	T/T	57 (62.0)	2.2 × 10⁴ (2.1 × 10³–1.5 × 10⁵) (n = 54)		5.8 × 10⁶ (6.0 × 10⁵–1.0 × 10⁷) (n = 56)
rs3212227	T/G	28 (30.4)	7.4 × 10³ (1.8 × 10³–4.7 × 10⁴)	NS	1.0 × 10⁷ (6.4 × 10⁶–1.0 × 10⁷) (n = 27)	0.029
G/T	G/G	7 (7.6)	4.3 × 10³ (2.1 × 10³–3.0 × 10⁴)		1.7 × 10⁶ (4.2 × 10⁵–1.0 × 10⁷)
IL28B	T/T	13 (14.1)	5.8 × 10³ (1.9 × 10³–1.3 × 10⁵) (n = 12)		2.8 × 10⁶ (4.0 × 10⁵–1.0 × 10⁷) (n = 12)
rs12979860	T/C	38 (41.3)	1.0 × 10⁴ (1.6 × 10³–1.3 × 10⁵)	NS	1.0 × 10⁷ (2.8 × 10⁶–1.0 × 10⁷) (n = 37)	NS
C/T	C/C	41 (44.6)	1.2 × 10⁴ (2.1 × 10³–8.2 × 10⁴) (n = 39)		8.6 × 10⁶ (8.4 × 10⁵–1.0 × 10⁷)
CCL2	G/G	3 (3.3)	2.4 × 10³ (0–9.4 × 10³)		1.0 × 10⁷ (9.2 × 10⁶–1.0 × 10⁷)
rs1024611	G/A	39 (42.4)	1.7 × 10⁴ (1.7 × 10³–5.8 × 10⁴) (n = 38)	NS	5.5 × 10⁶ (5.2 × 10⁵–1.0 × 10⁷) (n = 37)	NS
A/G	A/A	50 (54.3)	8.9 × 10³ (2.4 × 10³–1.4 × 10⁵) (n = 48)		8.5 × 10⁶ (1.6 × 10⁶–1.0 × 10⁷)
DC-SIGN	G/G	35 (38.0)	1.2 × 10⁴ (1.4 × 10³–1.2 × 10⁵) (n = 32)		1.0 × 10⁷ (1.3 × 10⁶–1.0 × 10⁷)
rs735240	G/A	37 (40.2)	1.1 × 10⁴ (2.4 × 10³–1.7 × 10⁵)	NS	7.6 × 10⁶ (1.1 × 10⁶–1.0 × 10⁷) (n = 36)	NS
A/G	A/A	20 (21.7)	7.4 × 10³ (1.7 × 10³–3.5 × 10⁴)		5.5 × 10⁶ (5.0 × 10⁵–1.0 × 10⁷) (n = 19)
TLR2	G/G	82 (89.1)	1.2 × 10⁴ (2.1 × 10³–1.1 × 10⁵) (n = 79)		7.8 × 10⁶ (1.2 × 10⁶–1.0 × 10⁷) (n = 80)
rs5743708	G/A	10 (10.9)	4.3 × 10³ (1.6 × 10³–3.8 × 10⁴)	NS	1.0 × 10⁷ (1.0 × 10⁶–1.0 × 10⁷)	NS
A/G	A/A	0 (0)	NA		NA
TLR4	T/T	0 (0)	NA		NA
rs4986791	T/C	9 (9.8)	2.0 × 10⁴ (2.8 × 10³–9.8 × 10⁴)	NS	1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷)	NS
C/T	C/C	83 (90.2)	1.0 × 10⁴ (2.0 × 10³–1.0 × 10⁵) (n = 80)		8.6 × 10⁶ (1.1 × 10⁶–1.0 × 10⁷) (n = 81)
TLR9	T/T	30 (32.6)	2.4 × 10⁴ (3.6 × 10³–2.6 × 10⁵) (n = 29)		1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷) (n = 29)
rs352140	T/C	47 (51.1)	8.4 × 10³ (2.1 × 10³–5.7 × 10⁴) (n = 45)	NS	5.7 × 10⁶ (8.4 × 10⁵–1.0 × 10⁷) (n = 46)	NS
C/T	C/C	15 (16.3)	7.9 × 10³ (1.1 × 10³–3.7 × 10⁴)		1.0 × 10⁷ (1.6 × 10⁶–1.0 × 10⁷)

Association between genotypes of examined polymorphisms and viremia and viruriain asymptomaticneonates with cCMV infection

Asymptomatic infants with cCMV infection(n = 19)
SNP, rs number SNP database (dbSNP) reference number (ID number). , alleles	Genotype	n = 19 n (%)	HCMV DNA (copies/mL) in blood	p-value p-value – for comparison between genotypes and viremia.	HCMV DNA (copies/mL) in urine	p-value p-value – for comparison between genotypes and viruria.
IL1B	A/A	2 (10.53)	1.4 × 10⁴ (9.9 × 10²–2.7 × 10⁴)		5.8 ×10⁶ (2.8 × 10⁶–8.8 × 10⁶)
rs16944	A/G	10 (52.63)	1.8 × 10³ (3.1 × 10²–4.0 × 10³)	NS	2.7 ×10⁵ (4.0 × 10⁴–3.2 × 10⁶)	NS
G/A	G/G	7 (36.84)	2.0 × 10³ (1.2 × 10³–2.1 × 10³) (n = 5)		6.9 ×10⁵ (1.4 × 10⁵–6.2 × 10⁶)
IL12B	T/T	14 (73.68)	1.8 × 10³ (1.0 × 10³–1.4 × 10⁴) (n = 12)		4.9 × 10⁵ (1.4 × 10⁵–4.6 × 10⁶)
rs3212227	T/G	4 (21.05)	2.0 × 10³ (0–3.8 × 10⁵)	NS	1.1 × 10⁷ (1.0 × 10⁶–4.0 × 10⁷)	NS
G/T	G/G	1 (5.26)	2.1 × 10³ (2.1 × 10³–2.1 × 10³)		1.7 × 10⁵ (1.7 × 10⁵–1.7 × 10⁵)
IL28B	T/T	2 (10.53)	1.9 × 10³ (1.2 × 10³–2.7 × 10³)		2.7 × 10⁵ (2.5 × 10⁵–2.9 × 10⁵)
rs12979860	T/C	6 (31.58)	1.1 × 10³ (0–1.6 × 10³)	NS	2.4× 10⁶ (1.7 × 10⁵–6.2 × 10⁶)	NS
C/T	C/C	11 (57.89)	2.1 × 10³ (2.0 × 10³–2.7 × 10⁴) (n = 9)		6.9× 10⁵ (4.0 × 10⁴–6.1 × 10⁶)
CCL2	G/G	0 (0.00)	NA		NA
rs1024611	G/A	9 (47.37)	2.4 × 10³ (9.5 × 10²–1.5 × 10⁴) (n = 8)	NS	2.5× 10⁵ (1.4 × 10⁵–3.2 × 10⁶)	NS
A/G	A/A	10 (52.63)	1.2 × 10³ (9.9 × 10²–2.1 × 10³) (n = 9)		1.7× 10⁶ (1.7 × 10⁵–6.1 × 10⁶)
DC-SIGN	G/G	7 (36.84)	3.1 × 10² (0–1.2 × 10³) (n = 5)		2.9× 10⁵ (3.3 × 10³–2.0 × 10⁷)
rs735240	G/A	6 (31.58)	2.0 × 10³ (1.6 × 10³–2.7 × 10⁴)	NS	2.6× 10⁶ (1.7 × 10⁵–6.2 × 10⁶)	NS
A/G	A/A	6 (31.58)	2.4 × 10³ (9.9 × 10²–3.2 × 10⁴)		1.1× 10⁶ (4.0 × 10⁴–2.8 × 10⁶)
TLR2	G/G	17 (89.47)	2.1 × 10³ (3.1 × 10²–2.7 × 10⁴)(n = 15)		2.0× 10⁶ (1.4 × 10⁵–6.1 × 10⁶)
rs5743708	G/A	2 (10.53)	1.4 × 10³ (1.2 × 10³–1.6 × 10³)	NS	2.3× 10⁵ (1.7 × 10⁵–2.9 × 10⁵)	NS
A/G	A/A	0 (0.00)	NA		NA
TLR4	T/T	0 (0.00)	NA		NA
rs4986791	T/C	2 (10.53)	2.0 × 10³ (0–4.0 × 10³)	NS	1.0 × 10⁷ (0–2.0 × 10⁷)	NS
C/T	C/C	17 (89.47)	2.0 × 10³ (9.9 × 10²–2.7 × 10⁴)(n = 15)		6.9× 10⁵ (1.7 × 10⁵–4.6 × 10⁶)
TLR9	T/T	7 (36.84)	1.4 × 10³ (0–4.0 × 10³) (n = 6)		2.9× 10⁵ (1.4 × 10⁵–2.0 × 10⁷)
rs352140	T/C	9 (47.37)	2.1 × 10³ (1.6 × 10³–1.7 × 10⁴)(n = 8)	NS	6.9× 10⁵ (1.7 × 10⁵–4.6 × 10⁶)	NS
C/T	C/C	3 (15.79)	3.1× 10² (0–2.7 × 10⁴)		3.2× 10⁶ (0–8.8 × 10⁶)

Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in newborn infants with cCMV infection

Newborn infants with cCMV infection (N = 92)
SNP, rs number SNP database (dbSNP) reference number (ID number). , alleles	Allele	n, (%)	HCMV DNA (copies/mL) in blood	p-value p-value – for comparison between alleles and viremia.	HCMV DNA (copies/mL) in urine	p-value p-value – for comparison between alleles and viruria.
IL1B	G	121 (65.76)	1.2 × 10⁴ (2.0 × 10³–1.7 × 10⁵) (n = 116)		1.0 ×10⁷ (1.1 × 10⁶–1.0 × 10⁷) (n = 118)
rs16944				NS		NS
G/A	A	63 (34.24)	7.6 × 10³ (2.4 × 10³–3.8 × 10⁴) (n = 62)		6.8 ×10⁶ (1.1 × 10⁶–1.0 × 10⁷) (n = 62)
IL12B	T	142 (77.17)	1.6 × 10⁴ (2.0 × 10³–1.3 × 10⁵) (n = 136)		7.0 ×10⁶ (1.0 × 10⁶–1.0 × 10⁷) (n = 139)
rs3212227				NS
G/T	G	42 (22.83)	4.8 × 10³ (2.1 × 10³–3.0 × 10⁴)		1.0 ×10⁷ (1.7 × 10⁶–1.0 × 10⁷) (n = 41)	NS
IL28B	C	120 (65.22)	1.1 × 10⁴ (2.1 × 10³–9.0 × 10⁴) (n = 116)		8.8 ×10⁶ (1.0 × 10⁶–1.0 × 10⁷) (n = 119)
rs12979860				NS		NS
C/T	T	64 (34.78)	8.1 × 10³ (1.6 × 10³–1.3 × 10⁵) (n = 62)		7.0 ×10⁶ (1.3 × 10⁶–1.0 × 10⁷) (n = 61)
CCL2	A	139 (75.54)	1.1 × 10⁴ (2.1 × 10³–1.3 × 10⁵) (n = 134)		7.0 ×10⁶ (1.1 × 10⁶–1.0 × 10⁷) (n = 137)
rs1024611				NS		NS
A/G	G	45 (24.46)	1.0 × 10⁴ (1.6 × 10³–4.9 × 10⁴) (n = 44)		9.2 ×10⁶ (8.4 × 10⁵–1.0 × 10⁷) (n = 43)
DC-SIGN	G	107 (58.15)	1.1 × 10⁴ (2.0 × 10³–1.3 × 10⁵) (n = 101)		9.6 ×10⁶ (1.3 × 10⁶–1.0 × 10⁷) (n = 106)
rs735240				NS		NS
A/G	A	77 (41.85)	9.4 × 10³ (2.1 × 10³–5.7 × 10⁴)		6.1 ×10⁶ (1.0 × 10⁶–1.0 × 10⁷) (n = 74)
TLR2	G	174 (94.57)	1.1 × 10⁴ (2.1 × 10³–1.0 × 10⁵) (n = 168)		8.6 ×10⁶ (1.1 × 10⁶–1.0 × 10⁷) (n = 170)
rs5743708				NS		NS
A/G	A	10 (5.43)	4.3 × 10³ (1.6 × 10³–3.8 × 10⁴)		1.0 ×10⁷ (1.0 × 10⁶–1.0 × 10⁷)
TLR4	C	175 (95.11)	1.0 × 10⁴ (2.1 × 10³–9.8 × 10⁴) (n = 169)		8.6 ×10⁶ (1.1 × 10⁶–1.0 × 10⁷) (n = 171)
rs4986791				NS		NS
C/T	T	9 (4.89)	2.0 × 10⁴ (2.8 × 10³–9.8 × 10⁴)		1.0 ×10⁷ (1.7 × 10⁶–1.0 × 10⁷)
TLR9	T	107 (58.15)	1.7 × 10³ (2.1 × 10³–1.5 × 10⁵) (n = 103)		9.2 ×10⁶ (1.1 × 10⁶–1.0 × 10⁷) (n = 104)
rs352140				NS		NS
C/T	C	77 (41.85)	7.9 × 10³ (1.7 × 10³–5.1 × 10⁴) (n = 75)		7.8 ×10⁶ (1.0 × 10⁶–1.0 × 10⁷) (n = 76)

Distribution of genotypes of examined SNPs in the study population of cCMV-infected infants and subgroups of symptomatic and asymptomatic newborn infants

SNP, rs number SNP database (dbSNP) reference number (ID number). , alleles	Genotype	cCMV-infected neonates(n = 92)	Symptomatic neonates (n = 73)	Asymptomatic neonates (n = 19)	p-value p-value for comparison between frequencies of genotypes and subgroups of symptomatic and asymptomatic newborn infants.

			Genotype frequencies, n (%)
IL1B	A/A	7 (7.61)	5 (6.85)	2 (10.53)
rs16944	A/G	49 (53.26)	39 (53.42)	10 (52.63)	NS
G/A	G/G	36 (39.13)	29 (39.73)	7 (36.84)
IL12B	T/T	57 (61.96)	43 (58.90)	14 (73.68)
rs3212227	T/G	28 (30.43)	24 (32.88)	4 (21.05)	NS
G/T	G/G	7 (7.61)	6 (8.22)	1 (5.26)
IL28B	T/T	13 (14.13)	11 (15.07)	2 (10.53)
rs12979860	T/C	38 (41.30)	32 (43.84)	6 (31.58)	NS
C/T	C/C	41 (44.57)	30 (41.09)	11 (57.89)
CCL2	G/G	3 (3.26)	3 (4.11)	0 (0.00)
rs1024611	G/A	39 (42.39)	30 (41.10)	9 (47.37)	NS
A/G	A/A	50 (54.35)	40 (54.79)	10 (52.63)
DC-SIGN	G/G	35 (38.04)	28 (38.36)	7 (36.84)
rs735240	G/A	37 (40.22)	31 (42.47)	6 (31.58)	NS
A/G	A/A	20 (21.74)	14 (19.17)	6 (31.58)
TLR2	G/G	82 (89.13)	65 (89.04)	17 (89.47)
rs5743708	G/A	10 (10.87)	8 (10.96)	2 (10.53)	NS
A/G	A/A	0 (0.00)	0 (0.00)	0 (0.00)
TLR4	T/T	0 (0.00)	0 (0.00)	0 (0.00)
rs4986791	T/C	9 (9.78)	7 (9.59)	2 (10.53)	NS
C/T	C/C	83 (90.22)	66 (90.41)	17 (89.47)
TLR9	T/T	30 (32.61)	23 (31.51)	7 (36.84)
rs352140	T/C	47 (51.09)	38 (52.05)	9 (47.37)	NS
C/T	C/C	15 (16.30)	12 (16.44)	3 (15.79)

Association between genotypes of examined polymorphisms and viremia and viruria in symptomatic infants with cCMV infection

Symptomatic infants with cCMV infection (n = 73)
SNP rs number SNP database (dbSNP) reference number (ID number). alleles	Genotype	n = 73 n (%)	HCMV DNA (copies/mL) in blood	p-value p-value – for comparison between genotypes and viremia.	HCMV DNA (copies/mL) in urine	p-value p-value – for comparison between genotypes and viruria.
	A/A	5 (6.85)	6.3 × 10³ (5.4 × 10³–1.1 × 10⁴)		6.5 × 10⁶ (5.4 × 10⁶–1.0 × 10⁷)
IL1B
rs16944	A/G	39 (53.42)	1.9 × 10⁴ (3.6 × 10³–1.1 × 10⁵) (n = 38)	NS	1.0 × 10⁷ (1.1 × 10⁶–1.0 × 10⁷) (n = 38)	NS
G/A
	G/G	29 (39.73)	3.0 × 10⁴ (2.8 × 10³–2.1 × 10⁵)		1.0 × 10⁷ (3.7 × 10⁶–1.0 × 10⁷) (n = 28)
	T/T	43 (58.90)	3.9 × 10⁴ (5.4 × 10³–1.9 × 10⁵) (n = 42)		1.0 × 10⁷ (1.3 × 10⁶–1.0 × 10⁷) (n = 42)
IL12B
rs3212227	T/G	24 (32.88)	9.4 × 10³ (2.4 × 10³–4.7 × 10⁴)	NS	1.0 × 10⁷ (9.2 × 10⁶–1.0 × 10⁷) (n = 23)	NS
G/T
	G/G	6 (8.22)	7.9 × 10³ (2.4 × 10³–3.0 × 10⁴)		2.3 × 10⁶ (1.6 × 10⁶–1.0 × 10⁷)
	T/T	11 (15.07)	2.2 × 10⁴ (3.3 × 10³–2.1 × 10⁵) (n = 10)		7.0 × 10⁶ (1.3 × 10⁶–1.0 × 10⁷) (n = 10)
IL28B
rs12979860	T/C	32 (43.84)	1.9 × 10⁴ (3.2 × 10³–1.4 × 10⁵)	NS	1.0 × 10⁷ (3.5 × 10⁶–1.0 × 10⁷) (n = 31)	NS
C/T
	C/C	30 (41.09)	1.6 × 10⁴ (3.9 × 10³–1.1 × 10⁵)		1.0 × 10⁷ (1.4 × 10⁶–1.0 × 10⁷)
	G/G	3 (4.11)	2.4 × 10³ (0–9.4 × 10³)		1.0 × 10⁷ (9.2 × 10⁶–1.0 × 10⁷)
CCL2
rs1024611	G/A	30 (41.10)	2.9 × 10⁴ (3.6 × 10³–1.1 × 10⁵)	NS	1.0 × 10⁷ (1.0 × 10⁶–1.0 × 10⁷) (n = 28)	NS
A/G
	A/A	40 (54.79)	1.2 × 10⁴ (3.9 × 10³–2.0 × 10⁵) (n = 39)		1.0 × 10⁷ (3.7 × 10⁶–1.0 × 10⁷)
	G/G	28 (38.36)	2.9 × 10⁴ (4.3 × 10³–1.5 × 10⁵) (n = 27)		1.0 × 10⁷ (4.2 × 10⁶–1.0 × 10⁷)
DC-SIGN
rs735240	G/A	31 (42.47)	1.2 × 10⁴ (3.6 × 10³–1.9 × 10⁵)	NS	1.0 × 10⁷ (1.4 × 10⁶–1.0 × 10⁷) (n = 30)	NS
A/G
	A/A	14(19.17)	1.3 × 10⁴ (2.8 × 10³–3.8 × 10⁴)		1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷) (n = 13)
	G/G	65 (89.04)	1.9 × 10⁴ (3.7 × 10³–1.7 × 10⁵) (n = 64)		1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷) (n = 63)
TLR2
rs5743708	G/A	8 (10.96)	5.0 × 10³ (2.8 × 10³–8.4 × 10⁴)	NS	1.0 × 10⁷ (5.5 × 10⁶–1.0 × 10⁷)	NS
A/G
	A/A	0 (0.00)	NA		NA
	T/T	0 (0.00)	NA		NA
TLR4
rs4986791	T/C	7 (9.59)	3.8 × 10⁴ (2.8 × 10³–1.1 × 10⁵)	NS	1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷)	NS
C/T
	C/C	66 (9×0.41)	1.4 × 10⁴ (3.8 × 10³–1.5 × 10⁵) (n = 65)		1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷) (n = 64)
TLR9	T/T	23 (31.51)	2.9 × 10⁴ (5.3 × 10³–2.6 × 10⁵)		1.0 × 10⁷ (5.5 × 10⁶–1.0 × 10⁷) (n = 22)
rs352140	T/C	38 (52.05)	1.4 × 10⁴ (2.8 × 10³–8.2 × 10⁴) (n = 37)	NS	1.0 × 10⁷ (1.1 × 10⁶–1.0 × 10⁷) (n = 37)	NS
C/T	C/C	12 (16.44)	8.6 × 10³ (3.0 × 10³–4.7 × 10⁴)		1.0 × 10⁷ (4.3 × 10⁶–1.0 × 10⁷)

Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in symptomatic newborn infants with cCMV infection_

Symptomatic newborn infants with cCMV infection (N = 73)
SNP, rs number SNP database (dbSNP) reference number (ID number). , alleles	Allele	Alleles, n (%)	HCMV (copies/DNA mL) in blood	p-value p-value – for comparison between alleles and viremia.	HCMV DNA (copies/mL) in urine	p-value p-value – for comparison between alleles and viruria.
IL1B	G	97 (66.44)	2.2 × 10⁴ (3.0 × 10³–1.9 × 10⁵) (n = 96)		1.0 × 10⁷ (2.9 × 10⁶–1.0 × 10⁷) (n = 94)
rs16944				NS		NS
G/A	A	49 (33.56)	1.1 × 10⁴ (3.8 × 10³–5.4 × 10⁴) (n = 48)		1.0 × 10⁷ (1.5 × 10⁶–1.0 × 10⁷) (n = 48)
IL12B	T	110 (75.34)	2.6 × 10⁴ (4.7 × 10³–1.8 × 10⁵)		1.0 × 10⁷ (1.4 × 10⁶–1.0 × 10⁷)
rs3212227			(n = 108)	0.037	(n = 107)	NS
G/T	G	36 (24.66)	9.4 × 10³ (2.4 × 10³–3.4 × 10⁴)		1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷) (n = 35)
IL28B	C	92 (63.01)	1.7 × 10⁴ (3.8 × 10³–1.2 × 10⁵)		1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷) (n = 91)
rs12979860				NS		NS
C/T	T	54 (36.99)	1.9 × 10⁴ (3.3 × 10³–1.8 × 10⁵) (n = 52)		1.0 × 10⁷ (1.6 × 10⁶–1.0 × 10⁷) (n = 51)

CCL2	A	110 (75.34)	1.9 × 10⁴ (3.9 × 10³–1.7 × 10⁵)		1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷)
rs1024611			(n = 108)	NS	(n = 108)	NS
A/G	G	36 (24.66)	1.6 × 10⁴ (2.0 × 10³–5.4 × 10⁴)		1.0 × 10⁷ (1.4 × 10⁶–1.0 × 10⁷) (n = 34)
DC-SIGN	G	87 (59.59)	2.8 × 10⁴ (3.9 × 10³–1.5 × 10⁵) (n = 85)		1.0 × 10⁷ (2.9 × 10⁶–1.0 × 10⁷) (n = 86)
rs735240				NS		NS
A/G	A	59 (40.41)	1.2 × 10⁴ (3.3 × 10³–8.2 × 10⁴)		1.0 × 10⁷ (1.5 × 10⁶–1.0 × 10⁷) (n = 56)
TLR2	G	138 (94.52)	1.7 × 10⁴ (3.7 × 10³–1.5 × 10⁵)		1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷)
rs5743708			(n = 136)	NS	(n = 134)	NS
A/G	A	8 (5.48)	5.0 × 10³ (2.8 × 10³–8.4 × 10⁴)		1.0 × 10⁷ (5.5 × 10⁶–1.0 × 10⁷)
TLR4 rs4986791	C	139 (95.21)	1.7 × 10⁴ (3.8 × 10³–1.5 × 10⁵) (n = 137)	NS	1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷) (n = 135)	NS
C/T	T	7 (4.79)	3.8 × 10⁴ (2.8 × 10³–1.1 × 10⁵)		1.0 × 10⁷ (1.7 × 10⁶–1.0 × 10⁷)
TLR9	T	84 (57.53)	2.8 × 10⁴ (3.8 × 10³–1.9 × 10⁵) (n = 83)		1.0 × 10⁷ (2.9 × 10⁶–1.0 × 10⁷) (n = 81)
rs352140				NS		NS
C/T	C	62 (42.47)	9.4 × 10³ (2.8 × 10³–5.8 × 10⁴) (n = 61)		1.0 × 10⁷ (1.6 × 10⁶–1.0 × 10⁷) (n = 61)

Association between single nucleotide polymorphisms and viral load in congenital cytomegalovirus infection

Figures & Tables

Figure 1

Maternal and neonatal demographic data and general characteristics of cCMV-infected infants, with subgroups of symptomatic and asymptomatic infants_

Allele frequencies of examined SNPs in the study population of cCMV-infected neonates and subgroups of symptomatic and asymptomatic neonates

Allele frequencies of examined SNPs in the study population in comparison to known European and Global reference populations from the Reference SNP (rs) Report

Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in asymptomatic newborn infants with cCMV infection

Association between genotypes of examined polymorphisms (SNPs) and viremia and viruria in newborn infants with cCMV infection_

Association between genotypes of examined polymorphisms and viremia and viruriain asymptomaticneonates with cCMV infection

Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in newborn infants with cCMV infection

Distribution of genotypes of examined SNPs in the study population of cCMV-infected infants and subgroups of symptomatic and asymptomatic newborn infants

Association between genotypes of examined polymorphisms and viremia and viruria in symptomatic infants with cCMV infection

Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in symptomatic newborn infants with cCMV infection_

Paradigm

My account