Table 1
Maternal and neonatal demographic data and general characteristics of cCMV-infected infants, with subgroups of symptomatic and asymptomatic infants.
| Characteristics | cCMV-infected infants (n = 92) | Symptomatic infants (n = 73) | Asymptomatic infants (n = 19) | p-valuea |
|---|---|---|---|---|
| Maternal age at delivery, (years) | 28 (25–31) | 29 (25–31) | 28 (25–31) | NS |
| Primipara, n (%) | 42 (45.65) | 33 (45.21) | 9 (47.37) | NS |
| Preterm delivery (<37Hbd), n (%) | 23 (25.00) | 19 (26.03) | 4 (21.05) | NS |
| Caesarean section, n (%) | 49 (54.44) | 45 (61.64) | 4 (21.05) | 0.006 |
| Prenatal HCMV screening, n (%) | 37 (40.22) | 27 (36.99) | 10 (52.63) | NS |
| Birth weight, (g) | (21702755 –3200) | (21502685 –3140) | (26803050 –3630) | 0.028 |
| Gestational age, (weeks) | 38 (37–39) | 38 (37–39) | 39 (37–40) | NS |
| Male, n (%) | 46 (50.00) | 36 (49.32) | 10 (52.63) | NS |
| Age at admission to NICU, Median (IQR), (days) | 16 (9–23) | 14 (9–21) | 22 (20–35) | <0.001 |
| Weight at admission to NICU, (g) | 3040 (2440–3450) | 2980 (2340–3320) | 3600 (2930–4310) | <0.001 |
| Age at first HCMV DNA detection in urine, (days) | 9 (4–15) | 8 (3–14) | 17 (8–20) | 0.002 |
Figure 1
HCMV VL in urine depending on genotypes of IL12B rs3212227 polymorphism. Bars in the box show the first and the third quartiles. Whisker charts represent the minimum to maximum values. Small box represents median value. p-value below 0.05 is statistically significant. Kruskal-Wallis ANOVA test was used. HCMV: human cytomegalovirus; VL: viral load.
Table 2
Association between genotypes of examined polymorphisms (SNPs) and viremia and viruria in newborn infants with cCMV infection.
| SNP, rs numbera, alleles | Genotype | n = 92, n (%) | HCMV DNA (copies/mL) in blood | p-valueb | HCMV DNA (copies/mL) in urine | p-valuec |
|---|---|---|---|---|---|---|
| IL1B | A/A | 7 (7.6) | 6.3 × 103 (3.8 × 103–2.7 × 104) | 6.5 × 106 (2.8 × 106–1.0 × 107) | ||
| rs16944 | A/G | 49 (53.3) | 8.6 × 103 (1.9 × 103–5.8 × 104) (n = 48) | NS | 7.8 × 106 (5.1 × 105–1.0 × 107) (n = 48) | NS |
| G/A | G/G | 36 (39.1) | 1.5 × 104 (2.0 × 103–2.0 × 105) (n = 34) | 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 35) | ||
| IL12B | T/T | 57 (62.0) | 2.2 × 104 (2.1 × 103–1.5 × 105) (n = 54) | 5.8 × 106 (6.0 × 105–1.0 × 107) (n = 56) | ||
| rs3212227 | T/G | 28 (30.4) | 7.4 × 103 (1.8 × 103–4.7 × 104) | NS | 1.0 × 107 (6.4 × 106–1.0 × 107) (n = 27) | 0.029 |
| G/T | G/G | 7 (7.6) | 4.3 × 103 (2.1 × 103–3.0 × 104) | 1.7 × 106 (4.2 × 105–1.0 × 107) | ||
| IL28B | T/T | 13 (14.1) | 5.8 × 103 (1.9 × 103–1.3 × 105) (n = 12) | 2.8 × 106 (4.0 × 105–1.0 × 107) (n = 12) | ||
| rs12979860 | T/C | 38 (41.3) | 1.0 × 104 (1.6 × 103–1.3 × 105) | NS | 1.0 × 107 (2.8 × 106–1.0 × 107) (n = 37) | NS |
| C/T | C/C | 41 (44.6) | 1.2 × 104 (2.1 × 103–8.2 × 104) (n = 39) | 8.6 × 106 (8.4 × 105–1.0 × 107) | ||
| CCL2 | G/G | 3 (3.3) | 2.4 × 103 (0–9.4 × 103) | 1.0 × 107 (9.2 × 106–1.0 × 107) | ||
| rs1024611 | G/A | 39 (42.4) | 1.7 × 104 (1.7 × 103–5.8 × 104) (n = 38) | NS | 5.5 × 106 (5.2 × 105–1.0 × 107) (n = 37) | NS |
| A/G | A/A | 50 (54.3) | 8.9 × 103 (2.4 × 103–1.4 × 105) (n = 48) | 8.5 × 106 (1.6 × 106–1.0 × 107) | ||
| DC-SIGN | G/G | 35 (38.0) | 1.2 × 104 (1.4 × 103–1.2 × 105) (n = 32) | 1.0 × 107 (1.3 × 106–1.0 × 107) | ||
| rs735240 | G/A | 37 (40.2) | 1.1 × 104 (2.4 × 103–1.7 × 105) | NS | 7.6 × 106 (1.1 × 106–1.0 × 107) (n = 36) | NS |
| A/G | A/A | 20 (21.7) | 7.4 × 103 (1.7 × 103–3.5 × 104) | 5.5 × 106 (5.0 × 105–1.0 × 107) (n = 19) | ||
| TLR2 | G/G | 82 (89.1) | 1.2 × 104 (2.1 × 103–1.1 × 105) (n = 79) | 7.8 × 106 (1.2 × 106–1.0 × 107) (n = 80) | ||
| rs5743708 | G/A | 10 (10.9) | 4.3 × 103 (1.6 × 103–3.8 × 104) | NS | 1.0 × 107 (1.0 × 106–1.0 × 107) | NS |
| A/G | A/A | 0 (0) | NA | NA | ||
| TLR4 | T/T | 0 (0) | NA | NA | ||
| rs4986791 | T/C | 9 (9.8) | 2.0 × 104 (2.8 × 103–9.8 × 104) | NS | 1.0 × 107 (1.7 × 106–1.0 × 107) | NS |
| C/T | C/C | 83 (90.2) | 1.0 × 104 (2.0 × 103–1.0 × 105) (n = 80) | 8.6 × 106 (1.1 × 106–1.0 × 107) (n = 81) | ||
| TLR9 | T/T | 30 (32.6) | 2.4 × 104 (3.6 × 103–2.6 × 105) (n = 29) | 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 29) | ||
| rs352140 | T/C | 47 (51.1) | 8.4 × 103 (2.1 × 103–5.7 × 104) (n = 45) | NS | 5.7 × 106 (8.4 × 105–1.0 × 107) (n = 46) | NS |
| C/T | C/C | 15 (16.3) | 7.9 × 103 (1.1 × 103–3.7 × 104) | 1.0 × 107 (1.6 × 106–1.0 × 107) |
Data are presented as median (IQR) or number (%). NS, not significant (p < 0.05).
cCMV, congenital cytomegalovirus; CCL2,C-C motif chemokine ligand 2; DC-SIGN, dendritic cell-specific ICAM-grabbing non-integrin; HCMV, human cytomegalovirus; IL, interleukin; IQR, interquartile range; NA, not applicable; SNP, single nucleotide polymorphism; TLR, toll-like receptor.
Table 3
Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in symptomatic newborn infants with cCMV infection.
| SNP, rs numbera, alleles | Allele | Alleles, n (%) | HCMV (copies/DNA mL) in blood | p-valueb | HCMV DNA (copies/mL) in urine | p-valuec |
|---|---|---|---|---|---|---|
| IL1B | G | 97 (66.44) | 2.2 × 104 (3.0 × 103–1.9 × 105) (n = 96) | 1.0 × 107 (2.9 × 106–1.0 × 107) (n = 94) | ||
| rs16944 | NS | NS | ||||
| G/A | A | 49 (33.56) | 1.1 × 104 (3.8 × 103–5.4 × 104) (n = 48) | 1.0 × 107 (1.5 × 106–1.0 × 107) (n = 48) | ||
| IL12B | T | 110 (75.34) | 2.6 × 104 (4.7 × 103–1.8 × 105) | 1.0 × 107 (1.4 × 106–1.0 × 107) | ||
| rs3212227 | (n = 108) | 0.037 | (n = 107) | NS | ||
| G/T | G | 36 (24.66) | 9.4 × 103 (2.4 × 103–3.4 × 104) | 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 35) | ||
| IL28B | C | 92 (63.01) | 1.7 × 104 (3.8 × 103–1.2 × 105) | 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 91) | ||
| rs12979860 | NS | NS | ||||
| C/T | T | 54 (36.99) | 1.9 × 104 (3.3 × 103–1.8 × 105) (n = 52) | 1.0 × 107 (1.6 × 106–1.0 × 107) (n = 51) | ||
| CCL2 | A | 110 (75.34) | 1.9 × 104 (3.9 × 103–1.7 × 105) | 1.0 × 107 (1.7 × 106–1.0 × 107) | ||
| rs1024611 | (n = 108) | NS | (n = 108) | NS | ||
| A/G | G | 36 (24.66) | 1.6 × 104 (2.0 × 103–5.4 × 104) | 1.0 × 107 (1.4 × 106–1.0 × 107) (n = 34) | ||
| DC-SIGN | G | 87 (59.59) | 2.8 × 104 (3.9 × 103–1.5 × 105) (n = 85) | 1.0 × 107 (2.9 × 106–1.0 × 107) (n = 86) | ||
| rs735240 | NS | NS | ||||
| A/G | A | 59 (40.41) | 1.2 × 104 (3.3 × 103–8.2 × 104) | 1.0 × 107 (1.5 × 106–1.0 × 107) (n = 56) | ||
| TLR2 | G | 138 (94.52) | 1.7 × 104 (3.7 × 103–1.5 × 105) | 1.0 × 107 (1.7 × 106–1.0 × 107) | ||
| rs5743708 | (n = 136) | NS | (n = 134) | NS | ||
| A/G | A | 8 (5.48) | 5.0 × 103 (2.8 × 103–8.4 × 104) | 1.0 × 107 (5.5 × 106–1.0 × 107) | ||
| TLR4 rs4986791 | C | 139 (95.21) | 1.7 × 104 (3.8 × 103–1.5 × 105) (n = 137) | NS | 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 135) | NS |
| C/T | T | 7 (4.79) | 3.8 × 104 (2.8 × 103–1.1 × 105) | 1.0 × 107 (1.7 × 106–1.0 × 107) | ||
| TLR9 | T | 84 (57.53) | 2.8 × 104 (3.8 × 103–1.9 × 105) (n = 83) | 1.0 × 107 (2.9 × 106–1.0 × 107) (n = 81) | ||
| rs352140 | NS | NS | ||||
| C/T | C | 62 (42.47) | 9.4 × 103 (2.8 × 103–5.8 × 104) (n = 61) | 1.0 × 107 (1.6 × 106–1.0 × 107) (n = 61) |
Data are presented as median (IQR) or number (%).
cCMV, congenital cytomegalovirus; CCL2, C-C motif chemokine ligand 2; DC-SIGN, dendritic cell-specific ICAM-grabbing non-integrin; HCMV, human cytomega lovirus; IL, interleukin; IQR, interquartile range; NS, not significant (p < 0.05); SNP, single nucleotide polymorphism; TLR, toll-like receptor.
Table S1
Distribution of genotypes of examined SNPs in the study population of cCMV-infected infants and subgroups of symptomatic and asymptomatic newborn infants
| SNP, rs numbera, alleles | Genotype | cCMV-infected neonates(n = 92) | Symptomatic neonates (n = 73) | Asymptomatic neonates (n = 19) | p-valueb |
|---|---|---|---|---|---|
| Genotype frequencies, n (%) | |||||
| IL1B | A/A | 7 (7.61) | 5 (6.85) | 2 (10.53) | |
| rs16944 | A/G | 49 (53.26) | 39 (53.42) | 10 (52.63) | NS |
| G/A | G/G | 36 (39.13) | 29 (39.73) | 7 (36.84) | |
| IL12B | T/T | 57 (61.96) | 43 (58.90) | 14 (73.68) | |
| rs3212227 | T/G | 28 (30.43) | 24 (32.88) | 4 (21.05) | NS |
| G/T | G/G | 7 (7.61) | 6 (8.22) | 1 (5.26) | |
| IL28B | T/T | 13 (14.13) | 11 (15.07) | 2 (10.53) | |
| rs12979860 | T/C | 38 (41.30) | 32 (43.84) | 6 (31.58) | NS |
| C/T | C/C | 41 (44.57) | 30 (41.09) | 11 (57.89) | |
| CCL2 | G/G | 3 (3.26) | 3 (4.11) | 0 (0.00) | |
| rs1024611 | G/A | 39 (42.39) | 30 (41.10) | 9 (47.37) | NS |
| A/G | A/A | 50 (54.35) | 40 (54.79) | 10 (52.63) | |
| DC-SIGN | G/G | 35 (38.04) | 28 (38.36) | 7 (36.84) | |
| rs735240 | G/A | 37 (40.22) | 31 (42.47) | 6 (31.58) | NS |
| A/G | A/A | 20 (21.74) | 14 (19.17) | 6 (31.58) | |
| TLR2 | G/G | 82 (89.13) | 65 (89.04) | 17 (89.47) | |
| rs5743708 | G/A | 10 (10.87) | 8 (10.96) | 2 (10.53) | NS |
| A/G | A/A | 0 (0.00) | 0 (0.00) | 0 (0.00) | |
| TLR4 | T/T | 0 (0.00) | 0 (0.00) | 0 (0.00) | |
| rs4986791 | T/C | 9 (9.78) | 7 (9.59) | 2 (10.53) | NS |
| C/T | C/C | 83 (90.22) | 66 (90.41) | 17 (89.47) | |
| TLR9 | T/T | 30 (32.61) | 23 (31.51) | 7 (36.84) | |
| rs352140 | T/C | 47 (51.09) | 38 (52.05) | 9 (47.37) | NS |
| C/T | C/C | 15 (16.30) | 12 (16.44) | 3 (15.79) |
Table S2
Allele frequencies of examined SNPs in the study population in comparison to known European and Global reference populations from the Reference SNP (rs) Report
| Allele frequencies, n (%) | Allele frequencies, % | ||||||
|---|---|---|---|---|---|---|---|
| IL1B | G | 320 (68.67) | 199 (70.57) | 121 (65.76) | 65.01 | 49.06 | |
| rs16944 | NS | ||||||
| G/A | A | 146 (31.33) | 83 (29.43) | 63 (34.24) | 34.99 | 50.94 | |
| IL12B | T | 352(75.54) | 210 (74.47) | 142 (77.17) | 77.73 | 64.10 | |
| rs3212227 | NS | ||||||
| G/T | G | 114 (24.46) | 72 (25.53) | 42 (22.83) | 22.27 | 35.90 | |
| IL28B | C | 309 (66.31) | 189 (67.02) | 120 (65.22) | 69.09 | 64.42 | |
| rs12979860 | NS | ||||||
| C/T | T | 157 (33.69) | 93 (32.98) | 64 (34.78) | 30.91 | 35.58 | |
| CCL2 | A | 344 (73.82) | 205 (72.70) | 139 (75.54) | 68.39 | 63.64 | |
| rs1024611 | NS | ||||||
| A/G | G | 122 (26.18) | 77(27.30) | 45 (24.46) | 31.61 | 36.36 | |
| DC-SIGN | G | 278 (59.66) | 171 (60.64) | 107 (58.15) | 56.76 | 64.70 | |
| rs735240 | NS | ||||||
| A/G | A | 188 (40.34) | 111 (39.36) | 77 (41.85) | 43.24 | 35.30 | |
| TLR2 | G | 441 (94.64) | 267 (94.68) | 174 (94.57) | 97.61 | 99.32 | |
| rs5743708 | NS | ||||||
| A/G | A | 25 (5.36) | 15 (5.32) | 10 (5.43) | 2.39 | 0.68 | |
| TLR4 | C | 440 (94.42) | 265 (93.97) | 175 (95.11) | 94.23 | 95.93 | |
| rs4986791 | NS | ||||||
| C/T | T | 26 (5.58) | 17 (6.03) | 9 (4.89) | 5.77 | 4.07 | |
| TLR9 | T | 269 (57.73) | 162 (57.45) | 107 (58.15) | 54.57 | 41.55 | |
| rs352140 | NS | ||||||
| C/T | C | 197 (42.27) | 120 (42.55) | 77 (41.85) | 45.43 | 58.45 | |
Table S3
Allele frequencies of examined SNPs in the study population of cCMV-infected neonates and subgroups of symptomatic and asymptomatic neonates
| SNP,rs numbera, alleles | Allele | cCMV-infected infants (n = 92) | Symptomatic infants (n = 73) | Asymptomatic infants (n = 19) | p-valueb |
|---|---|---|---|---|---|
| Allele frequencies, n (%) | |||||
| IL1B | G | 121 (65.76) | 97 (66.44) | 24 (63.16) | |
| rs16944 | NS | ||||
| G/A | A | 63 (34.24) | 49 (33.56) | 14 (36.84) | |
| IL12B | T | 142 (77.17) | 110 (75.34) | 32 (84.21) | |
| rs3212227 | NS | ||||
| G/T | G | 42 (22.83) | 36 (24.66) | 6 (15.79) | |
| IL28B | C | 120 (65.22) | 92 (63.01) | 28 (73.68) | |
| rs12979860 | NS | ||||
| C/T | T | 64 (34.78) | 54 (36.99) | 10 (26.32) | |
| CCL2 | A | 139 (75.54) | 110 (75.34) | 29 (76.32) | |
| rs1024611 | NS | ||||
| A/G | G | 45 (24.46) | 36 (24.66) | 9 (23.68) | |
| DC-SIGN | G | 107 (58.15) | 87 (59.59) | 20 (52.63) | |
| rs735240 | NS | ||||
| A/G | A | 77 (41.85) | 59 (40.41) | 18 (47.37) | |
| TLR2 | G | 174 (94.57) | 138 (94.52) | 36 (94.74) | |
| rs5743708 | NS | ||||
| A/G | A | 10 (5.43) | 8 (5.48) | 2 (5.26) | |
| TLR4 | C | 175 (95.11) | 139 (95.21) | 36 (94.74) | |
| rs4986791 | NS | ||||
| C/T | T | 9 (4.89) | 7 (4.79) | 2 (5.26) | |
| TLR9 | T | 107 (58.15) | 84 (57.53) | 23 (60.53) | |
| rs352140 | NS | ||||
| C/T | C | 77 (41.85) | 62 (42.47) | 15 (39.47) | |
Table S4
Association between genotypes of examined polymorphisms and viremia and viruria in symptomatic infants with cCMV infection
| SNP rs numbera alleles | Genotype | n = 73 n (%) | HCMV DNA (copies/mL) in blood | p-valueb | HCMV DNA (copies/mL) in urine | p-valuec |
|---|---|---|---|---|---|---|
| A/A | 5 (6.85) | 6.3 × 103 (5.4 × 103–1.1 × 104) | 6.5 × 106 (5.4 × 106–1.0 × 107) | |||
| IL1B | ||||||
| rs16944 | A/G | 39 (53.42) | 1.9 × 104 (3.6 × 103–1.1 × 105) (n = 38) | NS | 1.0 × 107 (1.1 × 106–1.0 × 107) (n = 38) | NS |
| G/A | ||||||
| G/G | 29 (39.73) | 3.0 × 104 (2.8 × 103–2.1 × 105) | 1.0 × 107 (3.7 × 106–1.0 × 107) (n = 28) | |||
| T/T | 43 (58.90) | 3.9 × 104 (5.4 × 103–1.9 × 105) (n = 42) | 1.0 × 107 (1.3 × 106–1.0 × 107) (n = 42) | |||
| IL12B | ||||||
| rs3212227 | T/G | 24 (32.88) | 9.4 × 103 (2.4 × 103–4.7 × 104) | NS | 1.0 × 107 (9.2 × 106–1.0 × 107) (n = 23) | NS |
| G/T | ||||||
| G/G | 6 (8.22) | 7.9 × 103 (2.4 × 103–3.0 × 104) | 2.3 × 106 (1.6 × 106–1.0 × 107) | |||
| T/T | 11 (15.07) | 2.2 × 104 (3.3 × 103–2.1 × 105) (n = 10) | 7.0 × 106 (1.3 × 106–1.0 × 107) (n = 10) | |||
| IL28B | ||||||
| rs12979860 | T/C | 32 (43.84) | 1.9 × 104 (3.2 × 103–1.4 × 105) | NS | 1.0 × 107 (3.5 × 106–1.0 × 107) (n = 31) | NS |
| C/T | ||||||
| C/C | 30 (41.09) | 1.6 × 104 (3.9 × 103–1.1 × 105) | 1.0 × 107 (1.4 × 106–1.0 × 107) | |||
| G/G | 3 (4.11) | 2.4 × 103 (0–9.4 × 103) | 1.0 × 107 (9.2 × 106–1.0 × 107) | |||
| CCL2 | ||||||
| rs1024611 | G/A | 30 (41.10) | 2.9 × 104 (3.6 × 103–1.1 × 105) | NS | 1.0 × 107 (1.0 × 106–1.0 × 107) (n = 28) | NS |
| A/G | ||||||
| A/A | 40 (54.79) | 1.2 × 104 (3.9 × 103–2.0 × 105) (n = 39) | 1.0 × 107 (3.7 × 106–1.0 × 107) | |||
| G/G | 28 (38.36) | 2.9 × 104 (4.3 × 103–1.5 × 105) (n = 27) | 1.0 × 107 (4.2 × 106–1.0 × 107) | |||
| DC-SIGN | ||||||
| rs735240 | G/A | 31 (42.47) | 1.2 × 104 (3.6 × 103–1.9 × 105) | NS | 1.0 × 107 (1.4 × 106–1.0 × 107) (n = 30) | NS |
| A/G | ||||||
| A/A | 14(19.17) | 1.3 × 104 (2.8 × 103–3.8 × 104) | 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 13) | |||
| G/G | 65 (89.04) | 1.9 × 104 (3.7 × 103–1.7 × 105) (n = 64) | 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 63) | |||
| TLR2 | ||||||
| rs5743708 | G/A | 8 (10.96) | 5.0 × 103 (2.8 × 103–8.4 × 104) | NS | 1.0 × 107 (5.5 × 106–1.0 × 107) | NS |
| A/G | ||||||
| A/A | 0 (0.00) | NA | NA | |||
| T/T | 0 (0.00) | NA | NA | |||
| TLR4 | ||||||
| rs4986791 | T/C | 7 (9.59) | 3.8 × 104 (2.8 × 103–1.1 × 105) | NS | 1.0 × 107 (1.7 × 106–1.0 × 107) | NS |
| C/T | ||||||
| C/C | 66 (9×0.41) | 1.4 × 104 (3.8 × 103–1.5 × 105) (n = 65) | 1.0 × 107 (1.7 × 106–1.0 × 107) (n = 64) | |||
| TLR9 | T/T | 23 (31.51) | 2.9 × 104 (5.3 × 103–2.6 × 105) | 1.0 × 107 (5.5 × 106–1.0 × 107) (n = 22) | ||
| rs352140 | T/C | 38 (52.05) | 1.4 × 104 (2.8 × 103–8.2 × 104) (n = 37) | NS | 1.0 × 107 (1.1 × 106–1.0 × 107) (n = 37) | NS |
| C/T | C/C | 12 (16.44) | 8.6 × 103 (3.0 × 103–4.7 × 104) | 1.0 × 107 (4.3 × 106–1.0 × 107) |
Data are presented as median (IQR- interquartile range) or number (%).
cCMV, congenital cytomegalovirus; CCL2, C-C motif chemokine ligand 2; DC-SIGN, dendritic cell-specific ICAM-grabbing non-integrin; HCMV, human cytomegalovirus; IL, Interleukin; NS, not significant (p < 0.05); NA, not applicable; SNP, single nucleotide polymorphism; TLR, Toll-like receptor.
Table S5
Association between genotypes of examined polymorphisms and viremia and viruriain asymptomaticneonates with cCMV infection
| SNP, rs numbera, alleles | Genotype | n = 19 n (%) | HCMV DNA (copies/mL) in blood | p-valueb | HCMV DNA (copies/mL) in urine | p-valuec |
|---|---|---|---|---|---|---|
| IL1B | A/A | 2 (10.53) | 1.4 × 104 (9.9 × 102–2.7 × 104) | 5.8 ×106 (2.8 × 106–8.8 × 106) | ||
| rs16944 | A/G | 10 (52.63) | 1.8 × 103 (3.1 × 102–4.0 × 103) | NS | 2.7 ×105 (4.0 × 104–3.2 × 106) | NS |
| G/A | G/G | 7 (36.84) | 2.0 × 103 (1.2 × 103–2.1 × 103) (n = 5) | 6.9 ×105 (1.4 × 105–6.2 × 106) | ||
| IL12B | T/T | 14 (73.68) | 1.8 × 103 (1.0 × 103–1.4 × 104) (n = 12) | 4.9 × 105 (1.4 × 105–4.6 × 106) | ||
| rs3212227 | T/G | 4 (21.05) | 2.0 × 103 (0–3.8 × 105) | NS | 1.1 × 107 (1.0 × 106–4.0 × 107) | NS |
| G/T | G/G | 1 (5.26) | 2.1 × 103 (2.1 × 103–2.1 × 103) | 1.7 × 105 (1.7 × 105–1.7 × 105) | ||
| IL28B | T/T | 2 (10.53) | 1.9 × 103 (1.2 × 103–2.7 × 103) | 2.7 × 105 (2.5 × 105–2.9 × 105) | ||
| rs12979860 | T/C | 6 (31.58) | 1.1 × 103 (0–1.6 × 103) | NS | 2.4× 106 (1.7 × 105–6.2 × 106) | NS |
| C/T | C/C | 11 (57.89) | 2.1 × 103 (2.0 × 103–2.7 × 104) (n = 9) | 6.9× 105 (4.0 × 104–6.1 × 106) | ||
| CCL2 | G/G | 0 (0.00) | NA | NA | ||
| rs1024611 | G/A | 9 (47.37) | 2.4 × 103 (9.5 × 102–1.5 × 104) (n = 8) | NS | 2.5× 105 (1.4 × 105–3.2 × 106) | NS |
| A/G | A/A | 10 (52.63) | 1.2 × 103 (9.9 × 102–2.1 × 103) (n = 9) | 1.7× 106 (1.7 × 105–6.1 × 106) | ||
| DC-SIGN | G/G | 7 (36.84) | 3.1 × 102 (0–1.2 × 103) (n = 5) | 2.9× 105 (3.3 × 103–2.0 × 107) | ||
| rs735240 | G/A | 6 (31.58) | 2.0 × 103 (1.6 × 103–2.7 × 104) | NS | 2.6× 106 (1.7 × 105–6.2 × 106) | NS |
| A/G | A/A | 6 (31.58) | 2.4 × 103 (9.9 × 102–3.2 × 104) | 1.1× 106 (4.0 × 104–2.8 × 106) | ||
| TLR2 | G/G | 17 (89.47) | 2.1 × 103 (3.1 × 102–2.7 × 104)(n = 15) | 2.0× 106 (1.4 × 105–6.1 × 106) | ||
| rs5743708 | G/A | 2 (10.53) | 1.4 × 103 (1.2 × 103–1.6 × 103) | NS | 2.3× 105 (1.7 × 105–2.9 × 105) | NS |
| A/G | A/A | 0 (0.00) | NA | NA | ||
| TLR4 | T/T | 0 (0.00) | NA | NA | ||
| rs4986791 | T/C | 2 (10.53) | 2.0 × 103 (0–4.0 × 103) | NS | 1.0 × 107 (0–2.0 × 107) | NS |
| C/T | C/C | 17 (89.47) | 2.0 × 103 (9.9 × 102–2.7 × 104)(n = 15) | 6.9× 105 (1.7 × 105–4.6 × 106) | ||
| TLR9 | T/T | 7 (36.84) | 1.4 × 103 (0–4.0 × 103) (n = 6) | 2.9× 105 (1.4 × 105–2.0 × 107) | ||
| rs352140 | T/C | 9 (47.37) | 2.1 × 103 (1.6 × 103–1.7 × 104)(n = 8) | NS | 6.9× 105 (1.7 × 105–4.6 × 106) | NS |
| C/T | C/C | 3 (15.79) | 3.1× 102 (0–2.7 × 104) | 3.2× 106 (0–8.8 × 106) |
Data are presented as median (IQR- interquartile range) or number (%).
cCMV, congenital cytomegalovirus; CCL2, C-C motif chemokine ligand 2; DC-SIGN, dendritic cell-specific ICAM-grabbing non-integrin; HCMV, human cytomegalovirus; IL, Interleukin; NS, not significant (p < 0.05); NA, not applicable; SNP, single nucleotide polymorphism; TLR, Toll-like receptor.
Table S6
Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in newborn infants with cCMV infection
| SNP, rs numbera, alleles | Allele | n, (%) | HCMV DNA (copies/mL) in blood | p-valueb | HCMV DNA (copies/mL) in urine | p-valuec |
|---|---|---|---|---|---|---|
| IL1B | G | 121 (65.76) | 1.2 × 104 (2.0 × 103–1.7 × 105) (n = 116) | 1.0 ×107 (1.1 × 106–1.0 × 107) (n = 118) | ||
| rs16944 | NS | NS | ||||
| G/A | A | 63 (34.24) | 7.6 × 103 (2.4 × 103–3.8 × 104) (n = 62) | 6.8 ×106 (1.1 × 106–1.0 × 107) (n = 62) | ||
| IL12B | T | 142 (77.17) | 1.6 × 104 (2.0 × 103–1.3 × 105) (n = 136) | 7.0 ×106 (1.0 × 106–1.0 × 107) (n = 139) | ||
| rs3212227 | NS | |||||
| G/T | G | 42 (22.83) | 4.8 × 103 (2.1 × 103–3.0 × 104) | 1.0 ×107 (1.7 × 106–1.0 × 107) (n = 41) | NS | |
| IL28B | C | 120 (65.22) | 1.1 × 104 (2.1 × 103–9.0 × 104) (n = 116) | 8.8 ×106 (1.0 × 106–1.0 × 107) (n = 119) | ||
| rs12979860 | NS | NS | ||||
| C/T | T | 64 (34.78) | 8.1 × 103 (1.6 × 103–1.3 × 105) (n = 62) | 7.0 ×106 (1.3 × 106–1.0 × 107) (n = 61) | ||
| CCL2 | A | 139 (75.54) | 1.1 × 104 (2.1 × 103–1.3 × 105) (n = 134) | 7.0 ×106 (1.1 × 106–1.0 × 107) (n = 137) | ||
| rs1024611 | NS | NS | ||||
| A/G | G | 45 (24.46) | 1.0 × 104 (1.6 × 103–4.9 × 104) (n = 44) | 9.2 ×106 (8.4 × 105–1.0 × 107) (n = 43) | ||
| DC-SIGN | G | 107 (58.15) | 1.1 × 104 (2.0 × 103–1.3 × 105) (n = 101) | 9.6 ×106 (1.3 × 106–1.0 × 107) (n = 106) | ||
| rs735240 | NS | NS | ||||
| A/G | A | 77 (41.85) | 9.4 × 103 (2.1 × 103–5.7 × 104) | 6.1 ×106 (1.0 × 106–1.0 × 107) (n = 74) | ||
| TLR2 | G | 174 (94.57) | 1.1 × 104 (2.1 × 103–1.0 × 105) (n = 168) | 8.6 ×106 (1.1 × 106–1.0 × 107) (n = 170) | ||
| rs5743708 | NS | NS | ||||
| A/G | A | 10 (5.43) | 4.3 × 103 (1.6 × 103–3.8 × 104) | 1.0 ×107 (1.0 × 106–1.0 × 107) | ||
| TLR4 | C | 175 (95.11) | 1.0 × 104 (2.1 × 103–9.8 × 104) (n = 169) | 8.6 ×106 (1.1 × 106–1.0 × 107) (n = 171) | ||
| rs4986791 | NS | NS | ||||
| C/T | T | 9 (4.89) | 2.0 × 104 (2.8 × 103–9.8 × 104) | 1.0 ×107 (1.7 × 106–1.0 × 107) | ||
| TLR9 | T | 107 (58.15) | 1.7 × 103 (2.1 × 103–1.5 × 105) (n = 103) | 9.2 ×106 (1.1 × 106–1.0 × 107) (n = 104) | ||
| rs352140 | NS | NS | ||||
| C/T | C | 77 (41.85) | 7.9 × 103 (1.7 × 103–5.1 × 104) (n = 75) | 7.8 ×106 (1.0 × 106–1.0 × 107) (n = 76) |
Data are presented as median (IQR- interquartile range) or number (%).
cCMV, congenital cytomegalovirus; CCL2,C-C motif chemokine ligand 2; DC-SIGN, dendritic cell-specific ICAM-grabbing non-integrin; HCMV, human cytomega
lovirus; IL, interleukin; NS, not significant (p < 0.05); SNP, single nucleotide polymorphism;; TLR, toll-like receptor.
Table S7
Association between alleles of examined polymorphisms (SNPs) and viremia and viruria in asymptomatic newborn infants with cCMV infection
| SNP, rs numbera, alleles | Allele | n, (%) | HCMV DNA (copies/mL) in blood | p-valueb | HCMV DNA (copies/mL) in urine | p-valuec |
|---|---|---|---|---|---|---|
| IL1B | G | 24 (63.16) | 2.0 × 103 (7.5 × 102–3.3 × 103) (n = 20) | 4.9 ×105 (1.4 × 105–5.3 × 106) | ||
| rs16944 | NS | NS | ||||
| G/A | A | 14 (36.84) | 1.8 × 103 (9.9 × 102–2.7 × 104) | 2.4 ×106 (1.7 × 105–6.1 × 106) | ||
| IL12B | T | 32 (84.21) | 1.8 × 103 (9.9 × 102–1.5 × 104) (n = 28) | 6.9 ×105 (1.4 × 105–5.3 × 106) | ||
| rs3212227 | NS | NS | ||||
| G/T | G | 6 (15.79) | 2.1 × 103 (0.0–4.0 × 103) | 1.0 ×106 (1.7 × 105–2.0 × 107) | ||
| IL28B | C | 28 (73.68) | 2.1 × 103 (6.5 × 102–2.7 × 104) (n = 24) | 1.3 ×106 (9.1 × 104–6.1 × 106) | ||
| rs12979860 | NS | NS | ||||
| C/T | T | 10 (26.32) | 1.2 × 103 (9.9 × 102–2.7 × 103) | 2.9 ×105 (2.5 × 105–2.8 × 106) | ||
| CCL2 | A | 29 (76.32) | 1.8 × 103 (9.9 × 102–4.0 × 103) (n = 26) | 6.9 ×105 (1.7 × 105–6.1 × 106) | ||
| rs1024611 | NS | NS | ||||
| A/G | G | 9 (23.68) | 2.4 × 103 (9.5 × 102–1.5 × 104) (n = 8) | 2.5 ×105 (1.4 × 105–3.2 × 106) | ||
| DC-SIGN | G | 20 (52.63) | 1.2 × 103 (1.5 × 102–3.0 × 103) (n = 16) | 4.9 ×105 (1.4 × 105–7.5 × 106) | ||
| rs735240 | NS | NS | ||||
| A/G | A | 18 (47.37) | 2.1 × 103 (1.2 × 103–3.2 × 104) | 1.3 ×106 (1.7 × 105–4.6 × 106) | ||
| TLR2 | G | 36 (94.74) | 2.0 × 103 (6.5 × 102–1.5 × 104) (n = 32) | 1.3 ×106 (1.4 × 105–6.1 × 106) | ||
| rs5743708 | NS | NS | ||||
| A/G | A | 2 (5.26) | 1.4 × 103 (1.2 × 103–1.6 × 103) | 2.3 ×105 (1.7 × 105–2.9 × 105) | ||
| TLR4 | C | 36 (94.74) | 2.0 × 103 (9.9 × 102–1.5 × 104) (n = 32) | 6.9 ×105 (1.5 × 105–5.3 × 106) | ||
| rs4986791 | NS | NS | ||||
| C/T | T | 2 (5.26) | 2.0 × 103 (0–4.0 × 103) | 1.0 ×107 (0–2.0 × 107) | ||
| TLR9 | T | 23 (60.53) | 1.8 × 103 (1.0 × 103–4.0 × 103) (n = 20) | 2.9 ×105 (1.4 × 105–6.1 × 106) | ||
| rs352140 | NS | NS | ||||
| C/T | C | 15 (39.47) | 2.0 × 103 (3.1 × 102–2.7 × 104) (n = 14) | 2.8 ×106 (4.0 × 104–6.1 × 106) |
Data are presented as median (IQR- interquartile range) or number (%).
cCMV, congenital cytomegalovirus; CCL2, C-C motif chemokine ligand 2; DC-SIGN, dendritic cell-specific ICAM-grabbing non-integrin; HCMV, human cytomega lovirus; IL, interleukin; NS, not significant (p < 0.05); SNP, single nucleotide polymorphism; TLR, toll-like receptor.