Fig 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.
Experimental and predicted response for calcium biosorption using Virgibacillus pantothenticus_
| Run order | A Initial calcium concentration | B Contact time | C Biomass dosage | Biosorption capacity |
|---|---|---|---|---|
| 1 | 150 | 60 | 1.0 | 540.47 |
| 2 | 100 | 60 | 1.5 | 506.53 |
| 3 | 100 | 60 | 1.5 | 507.36 |
| 4 | 50 | 60 | 1.0 | 407.77 |
| 5 | 100 | 60 | 1.5 | 517.87 |
| 6 | 50 | 40 | 1.5 | 300.87 |
| 7 | 50 | 80 | 1.5 | 432.80 |
| 8 | 50 | 60 | 2.0 | 301.13 |
| 9 | 150 | 60 | 2.0 | 451.23 |
| 10 | 150 | 40 | 1.5 | 452.42 |
| 11 | 100 | 80 | 1.0 | 520.87 |
| 12 | 100 | 60 | 1.5 | 514.87 |
| 13 | 100 | 40 | 2.0 | 314.55 |
| 14 | 150 | 80 | 1.5 | 559.02 |
| 15 | 100 | 60 | 1.5 | 529.02 |
| 16 | 100 | 80 | 2.0 | 439.98 |
| 17 | 100 | 40 | 1.0 | 417.93 |
ANOVA results for calcium biosorption parameters_
| Source | Sum of squares | df | Mean square | F value | p-value | Significance |
|---|---|---|---|---|---|---|
| Model | 1.121E + 005 | 9 | 12459.59 | 236.49 | < 0.0001 | *** |
| A | 39379.40 | 1 | 39379.40 | 747.46 | < 0.0001 | *** |
| B | 27332.39 | 1 | 27332.39 | 518.79 | < 0.0001 | *** |
| C | 18064.25 | 1 | 18064.25 | 342.88 | < 0.0001 | *** |
| AB | 151.54 | 1 | 151.54 | 2.88 | 0.1337/ | |
| AC | 75.69 | 1 | 75.69 | 1.44 | 0.2697/ | |
| BC | 126.45 | 1 | 126.45 | 2.40 | 0.1653/ | |
| A2 | 6217.97 | 1 | 6217.97 | 118.02 | < 0.0001 | *** |
| B2 | 6820.04 | 1 | 6820.04 | 129.45 | < 0.0001 | *** |
| C2 | 11189.61 | 1 | 11189.61 | 212.39 | < 0.0001 | *** |
| Residual | 368.79 | 7 | 52.68 | / | / / | |
| Lack of Fit | 33.95 | 3 | 11.32 | 0.14 | 0.9340/ | |
| Pure Error | 334.84 | 4 | 83.71 | / | / | / |
| Cor Total | 1.125E + 005 | 16 | / | / | / | / |
| R2 | 0.9968/ | / | / | / | / | |
| Adj: R2 | 0.9928/ | / | / | / | / |
Physiological and biochemical tests and sugar fermentation tests results of strain N3-4_
| Physiological and biochemical characterization experiments | Sugar fermentation test | ||
|---|---|---|---|
| Indole test | + | Glucose | + |
| Citrate salt test | - | Fructose | + |
| Methyl Red test | + | Xylose | + |
| V-P test | + | Rhamnose | + |
| Gelatin hydrolysis test | + | Sucrose | + |
| Starch hydrolysis test | - | Arabinose | + |
| / | / | Marshmallow | - |
| / | / | Mannitol | + |