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Molecular Detection of a Pathogenic Entamoeba among Symptomatic Children in Eastern Kurdistan of Iraq Cover

Molecular Detection of a Pathogenic Entamoeba among Symptomatic Children in Eastern Kurdistan of Iraq

Open Access
|Mar 2024

Figures & Tables

Fig. 1.

The Lugol’s iodine wet mount of a stool sample showed cystic stage of Entamoeba species (blue arrows) applying a light microscope at a magnification of 40×.
The Lugol’s iodine wet mount of a stool sample showed cystic stage of Entamoeba species (blue arrows) applying a light microscope at a magnification of 40×.

Fig. 2.

Shows agarose gel electrophoresis for nested PCR products stained with 0.2 mg/ml ethidium bromide, using primers species specific for Entamoeba histolytica. Positive samples reveal 439 bp bands at lanes 2, 5, 8, 10–16, 19, and 21.
Lane 23 represented positive control, while lane 22 represented negative control, and lane 1 is the 100 bp DNA marker.
Shows agarose gel electrophoresis for nested PCR products stained with 0.2 mg/ml ethidium bromide, using primers species specific for Entamoeba histolytica. Positive samples reveal 439 bp bands at lanes 2, 5, 8, 10–16, 19, and 21. Lane 23 represented positive control, while lane 22 represented negative control, and lane 1 is the 100 bp DNA marker.

Potential risk factors associated with Entamoeba species infection among symptomatic children_

VariableFrequencyPositiveNegative
Number (%)
Gender
Male179 (55.42)33 (18.4)146 (81.6)
Female144 (44.58)25 (17.4)119 (82.6)
p-value0.884
Residency
Urban164 (50.8)22 (13.4)142 (86.6)
Rural159 (49.2)36 (22.6)123 (77.4)
p-value0.042*
Water source
Tap water159 (49.2)33 (20.8)126 (79.2)
Bottled water164 (50.8)25 (15.2)139 (84.8)
p-value0.246
Age group (years)
< 15.0 (1.5)0.0 (0.0)5.0 (100)
1–3141 (43.7)33 (23.4)108 (76.6)
4–6113 (35)16 (14.2)97 (85.8)
7–961 (18.9)7.0 (11.5)54 (88.5)
≥ 103.0 (0.9)2.0 (66.7)1.0 (33.3)
p-value0.032*
Total323 (100)58 (18)265 (82)

Potential risk factors associated with Entamoeba histolytica infection among microscopic positive symptomatic children_

VariableFrequencyPositiveNegative
Number (%)
Gender
Male33 (18.4)3.0 (1.7)30 (51.57)
Female25 (17.4)15 (10.4)10 (17.24)
p-value0.001**
Residency
Urban22 (13.4)5.0 (3.0)17 (29.31)
Rural36 (22.6)13 (8.2)23 (39.65)
p-value0.045*
Water source
Tap water33 (20.8)11 (6.9)22 (37.93)
Bottled water25 (15.2)7.0 (4.3)18 (31.03)
p-value0.299
Age group (years)
< 10.0 (0.0)0.0 (0.0)0.0 (0.0)
1–333 (23.4)14 (9.9)19 (32.75)
4–616 (14.2)1.0 (0.9)15 (25.86)
7–97.0 (11.5)2.0 (3.3)5.0 (8.62)
≥ 102.0 (66.7)1.0 (33.3)1.0 (1.72)
p-value0.004**
Total58 (18)18 (31)40 (69)
DOI: https://doi.org/10.33073/pjm-2024-010 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 99 - 105
Submitted on: Sep 25, 2023
Accepted on: Jan 27, 2024
Published on: Mar 4, 2024
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2024 Sham Jamil Abdullah, Shahnaz Abdulkader Ali, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.