Biocontrol and Growth Promotion Potential of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani that Causes Tobacco Target Spot Disease Cover

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Biocontrol and Growth Promotion Potential of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani that Causes Tobacco Target Spot Disease

Polish Journal of Microbiology

Volume 73 (2024): Issue 1 (March 2024)

By: Ning Huang, Xin Jin, Jin-Tao Wen, Yi-Fei Zhang, Xu Yang, Guang-Yu Wei, Yi-Kun Wang and Min Qin

Open Access

|Mar 2024

Figures & Tables

Growth curve of Bacillus subtilis CTXW 7-6-2 and its antifungal activity against Rhizoctonia solani.
A) CTXW 7-6-2 growth curve;
B) a: colony morphology of the control group, b: colony morphology after confrontation with the bacteria, c: sterile fermentation broth treatment group without high-temperature treatment, d: high-temperature sterile fermented broth treatment group; e: crude lipopeptide treatment group, f: volatile compounds treatment group.
The red box indicates the back of the colony. — Growth curve of Bacillus subtilis CTXW 7-6-2 and its antifungal activity against Rhizoctonia solani. A) CTXW 7-6-2 growth curve; B) a: colony morphology of the control group, b: colony morphology after confrontation with the bacteria, c: sterile fermentation broth treatment group without high-temperature treatment, d: high-temperature sterile fermented broth treatment group; e: crude lipopeptide treatment group, f: volatile compounds treatment group. The red box indicates the back of the colony.

Hypha morphology and cell ultrastructure of Rhizoctonia solani after treatment with Bacillus subtilis CTXW 7-6-2. A) Scanning electron microscopy observation of mycelium morphology; B) Ultrastructure of hyphal cells observed using transmission electron microscopy. The arrows in the figure indicate cell wall (cw), plasma membrane (pm), and mitochondria (m). a: the control hyphae, b: mycelia of the treated group

Effects of A) nutrient broth medium and B) CTXW 7-6-2 fermentation broth on seedling growth of Yunyan no. 87.

Expression of genes involved in tobacco disease course induced by Bacillus subtilis CTXW 7-6-2.

Antifungal activity of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani_

Classes	Control (cm)	Treatment (cm)	Inhibition ratio (%)
Antagonistic bacteria confront pathogenic fungi	7.11 ± 0.13	0.43 ± 0.03	94.02 ± 0.41^a
Sterile fermentation broth without high-temperature treatment		3.23 ± 0.06	54.63 ± 0.89^c
High-temperature processing of sterile fermentation broth		3.49 ± 0.14	50.88 ± 1.92^c
100 mg/l crude lipopeptide	5.28 ± 0.01	0.23 ± 0.21	74.88 ± 4.03^b
Volatile compounds	6.66 ± 0.26	0.23 ± 0.12	96.62 ± 1.77^a

Effects of CTXW 7-6-2 fermentation on the growth of tobacco seedlings_

Treat	CTXW 7-6-2	Nutrient broth
Leaf length (cm)	7.59 ± 0.29^a	4.44 ± 0.28^b
Leaf width (cm)	5.41 ± 0.31^a	3.25 ± 0.23^b
Leaf number (piece)	4.67 ±0.33^a	4.00 ± 0.00^a
Whole plant fresh weight (g)	2.01± 0.13^a	0.60 ± 0.0064^b
Root number	26.33 ± 0.88^a	24.33 ± 0.88^a
Root length (cm)	6.31 ± 0.37^b	10.41 ± 0.85^a
Dry weight of root (g)	0.17 ± 0.0025^a	0.091 ± 0.0041^b

DOI: https://doi.org/10.33073/pjm-2024-004 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331

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Language: English

Page range: 29 - 38

Submitted on: Sep 15, 2023

Accepted on: Dec 19, 2023

Published on: Mar 4, 2024

Published by: Polish Society of Microbiologists

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

tobacco target spot,

plant growth promotion,

biocontrol potential

Related subjects:

Microbiology and virology

© 2024 Ning Huang, Xin Jin, Jin-Tao Wen, Yi-Fei Zhang, Xu Yang, Guang-Yu Wei, Yi-Kun Wang, Min Qin, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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