Have a personal or library account? Click to login
Insights into Genomic Features and Potential Biotechnological Applications of Bacillus halotolerans Strain HGR5 Cover

Insights into Genomic Features and Potential Biotechnological Applications of Bacillus halotolerans Strain HGR5

Polish Journal of Microbiology
Volume 72 (2023): Issue 4 (December 2023)
By: Bilal Yahiaoui,  Hadjira Bounabi,  Amine M. Boukerb and  Meriem Gasmi  
Open Access
|Dec 2023

Figures & Tables

Fig. 1.

Antagonism effect of the nineteen selected strains based on their enzymatic activities. Control on the top left followed by each bacterial strain to the bottom right of each panel: HGG11, OTG3’> OTG9’> OTG6, OTR1, OTR2, OTK3, OTK1, OTK4, OTK8, OTK9, HGG7, HGG9, HGG15, HGG16, HGR5, HGK5, HGK11, HGK1, against a) Alternaria alternata; b) Fusarium gramine arum; c) Phytophthora infestans.
Antagonism effect of the nineteen selected strains based on their enzymatic activities. Control on the top left followed by each bacterial strain to the bottom right of each panel: HGG11, OTG3’> OTG9’> OTG6, OTR1, OTR2, OTK3, OTK1, OTK4, OTK8, OTK9, HGG7, HGG9, HGG15, HGG16, HGR5, HGK5, HGK11, HGK1, against a) Alternaria alternata; b) Fusarium gramine arum; c) Phytophthora infestans.

Fig. 2.

Whole-genome map of Bacillus halotolerans HGR5 generated by CGview. The genome map consists of six different circles from the inner to the outer circle: 1) measuring scale, 2) GC skew, 3) G + C content, 4) contig positions, 5) forward CDS, and 6) reverse CDS.
Whole-genome map of Bacillus halotolerans HGR5 generated by CGview. The genome map consists of six different circles from the inner to the outer circle: 1) measuring scale, 2) GC skew, 3) G + C content, 4) contig positions, 5) forward CDS, and 6) reverse CDS.

Fig. 3.

Phylogenetic analysis by maximum likelihood method based on the 16S rRNA gene sequences of HGR5 and its related Bacillus species. Accession numbers are shown after each strain ID. Bootstrapping was performed 1,000 times.
Phylogenetic analysis by maximum likelihood method based on the 16S rRNA gene sequences of HGR5 and its related Bacillus species. Accession numbers are shown after each strain ID. Bootstrapping was performed 1,000 times.

Fig. 4.

GO classifications of the genome sequence of Bacillus halotolerans HGR5. Bar chart represents the number of genes which were assigned a known GO function by the eggNOG-mapper tool.
GO classifications of the genome sequence of Bacillus halotolerans HGR5. Bar chart represents the number of genes which were assigned a known GO function by the eggNOG-mapper tool.

Fig. 5.

Esterases activities of Bacillus halotolerans HGR5. a) lipolytic activity on rhodamine B/coconut oil agar, b) lipolytic activity on tributyrin agar, c) biodegradation of PLCD.
Esterases activities of Bacillus halotolerans HGR5. a) lipolytic activity on rhodamine B/coconut oil agar, b) lipolytic activity on tributyrin agar, c) biodegradation of PLCD.

Fig. 6.

Growth curve of Bacillus halotolerans HGR5 under various conditions of stress. a) growth curve under various temperatures, b) growth curve under different pH, c) growth curve under increasing concentrations of NaCl. The error bars show the average standard deviation of each treatment, which was replicated three times.
Growth curve of Bacillus halotolerans HGR5 under various conditions of stress. a) growth curve under various temperatures, b) growth curve under different pH, c) growth curve under increasing concentrations of NaCl. The error bars show the average standard deviation of each treatment, which was replicated three times.

BGCs encoding secondary metabolites discovered by the AntiSMASH server in the Bacillus halotolerans HGR5 genome along with the closest biosynthetic gene clusters detected by ClusterKnownBlast and listed in the MIBiG database_

ClusterPredicted size (bp)BGC typeCompoundMIBiG accessionSimilarityClosest strain
141,419otherbacilycinBGC0001184Bacillus velezensis FZB42100%
221,613sactipeptidesubtilosin ABGC0000602Bacillus subtilis subsp. spizizenii ATCC® 6633™100%
347,140NPRSbacillibactinBGC0000309Bacillus subtilis subsp. subtilis str. 168100%
4106,121NRPS, transAT-PKSbacillaeneBGC0001089Bacillus velezensis FZB42100%
579,855NRPSfengycinBGC0001095Bacillus velezensis FZB4280%
NRPS, transAT-PKSmycosubtilinBGC0001103Bacillus subtilis subsp. spizizenii ATCC® 6633™100%
641,098T3PKS----
721,899terpene----
865,396NRPSsurfactinBGC0000433Bacillus velezensis FZB4286%
920,807terpene----
1051,225PKSmyxovirescin A1BGC0001025Myxococcus xanthus DK 162213%
macrolactin HBGC0000181Bacillus velezensis FZB4240%
1132,261PKSmacrobrevinBGC0001470Brevibacillus sp. Leaf18226%
phormidolideBGC0001350Leptolyngbya sp. ISBN3-Nov-94-821%
1213,175NRPSfengycinBGC0001095Bacillus velezensis FZB4220%
plipastatinBGC0000407Bacillus subtilis subsp. subtilis38%
1310,326NRPSplipastatinBGC0000407Bacillus subtilis subsp. subtilis23%
fengycinBGC0001095Bacillus velezensis FZB4220%
147,314PKSbryostatinBGC0000174Candidatus Endobugula sertula100%
DOI: https://doi.org/10.33073/pjm-2023-045 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Journal RSS Feed
Language: English
Page range: 477 - 490
Submitted on: Aug 18, 2023
Accepted on: Oct 27, 2023
Published on: Dec 16, 2023
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
genome mining,
hydrolytic enzymes,
secondary metabolites
Related subjects:
Life sciences,
Microbiology and virology

© 2023 Bilal Yahiaoui, Hadjira Bounabi, Amine M. Boukerb, Meriem Gasmi, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Previous articleVolume 72 (2023): Issue 4 (December 2023)Next article