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Diagnostic Efficacy of Photostimulated Chemiluminescence Assay for Detecting Anti-HIV Antibodies: A Retrospective Study Cover

Diagnostic Efficacy of Photostimulated Chemiluminescence Assay for Detecting Anti-HIV Antibodies: A Retrospective Study

Open Access
|Dec 2023

Figures & Tables

Table I

Coefficient of variation of the intra test for photostimulated chemiluminescence assay.

IndicatorLow S/COHigh S/CONegative S/CO
Mean3.73415.4950.461
SD0.0630.2680.014
CV1.68%1.73%3.03%

1 S/CO – signal value/cut-off value, CV – coefficient of variation,

SD – standard deviation

Table II

Coefficient of variation of the inter test for photostimulated chemiluminescence assay.

IndicatorLow S/COHigh S/CONegative S/CO
Mean4.01816.2840.474
SD0.0650.3650.016
CV1.63%2.24%3.38%

1 S/CO – signal value/cut-off value, CV – coefficient of variation, SD – standard deviation

Table III

Agreement of inter-test measure photostimulated chemiluminescence assay.

LICA500 in our hospitalLICA500 in Taizhou People’s HospitalAgreement
PositiveNegative
Positive100100%
Negative010100%
Table IV

Distribution ranges of COI values and WB test results in photostimulated chemiluminescence assay.

COIPhotostimulated chemiluminescence assay positiveWB positiveWB negative
  1.0–10.026125
10.0–20.0  2  0  2
20.0–30.0  2  0  2
30.0–40.0  2  2  0
> 40.04747  0
Total795029

1 COI – cut-off index, WB – Western blotting

Fig. 1.

ROC curve of photostimulated chemiluminescence assay.

Table V

CV values for three years of in-house quality control.

YearCV%
20203.61%
20213.36%
20223.67%
Mean3.55%

1 CV – coefficient of variation

DOI: https://doi.org/10.33073/pjm-2023-043 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 461 - 466
Submitted on: Jul 30, 2023
Accepted on: Oct 27, 2023
Published on: Dec 16, 2023
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2023 Jianxiang Han, Yong Wang, Bei Wang, Huacheng Tong, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.