Comparison of bacteria identified by the BioFire® FilmArray® Pneumonia plus Panel (PNP) and the conventional quantitative culture methods (CM) in 27 bacteriologically positive BAL samples_
| Bacterium | Conventional quantitative culture methods (CM) | BioFire® FilmArray® Pneumonia plus Panel (PNP) |
|---|---|---|
| Total number of bacteria detected* | Total number of bacteria detected | |
| Enterobacter cloacae complex** | 1 (0) | 2 |
| Escherichia coli** | 7 (4) | 8 |
| Haemophilus influenzae | 4 (3) | 4 |
| Klebsiella oxytoca | 1 (1) | 1 |
| Klebsiella pneumoniae group | 3 (2) | 3 |
| Moraxella (Branhamella) catarrhalis** | 0 (0) | 1 |
| Proteus spp. | 3 (1) | 3 |
| Pseudomonas aeruginosa**/*** | 5 (3) | 5 |
| Serratia marcescens** | 0 (0) | 1 |
| Staphylococcus aureus** | 9 (4) | 10 |
| Streptococcus agalactiae | 1 (1) | 1 |
| Streptococcus pneumoniae** | 2 (2) | 3 |
| Total number | 36 (21) | 42 |
| Resistance genes | ||
| CTX-M | 1# | 1 |
| IMP | 0 | 0 |
| KPC | 0 | 0 |
| NDM | 0 | 0 |
| VIM | 0 | 0 |
| OXA-48-like | 0 | 0 |
| mecA/C and MREJ | 0 | 0 |
| Total number | 1 | 1 |
Performance of quantitative growth of fungi in conventional quantitative culture method (CM)_
| Name of fungi | Quantity (CFU/ml) | |||
|---|---|---|---|---|
| 102-103 | 103-104 | 104-105 | > 105 | |
| Aspergillus fumigatus | 4 | 3 | 0 | 0 |
| Candida glabrata | 5 | 8 | 0 | 0 |
| Candida albicans | 6 | 5 | 1 | 0 |
| Candida tropicalis | 2 | 2 | 0 | 0 |
| Candida dubliniensis | 3 | 0 | 0 | 0 |
| Kluyveromyces marxianus (formerly Candida kefyr) | 1 | 0 | 0 | 0 |
| Pichia kudriavzevii (formerly Candida krusei) | 0 | 1 | 0 | 0 |
| Saccharomyces cerevisiae | 1 | 0 | 0 | 0 |
| Penicillium spp. | 1 | 0 | 0 | 0 |
| Total number | 23 | 19 | 1 | 0 |
Comparison of conventional quantitative culture method (CM) and the BioFire® FilmArray® Pneumonia plus Panel (PNP)_ Overall sensitivity, specificity, accuracy, and positive and negative predictive values_
| Methods | Samples | TP | FP | FN | TN | SENS | SP | ACC | PPV | NPV | FPR | FNR | AUC | SE | P |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PNP vs. comb | BAL | 27 | 0 | 1 | 51 | 0.964 | 1.000 | 0.987 | 1.000 | 0.981 | 0 | 0.036 | 0.982 | 0.02 | 0.0000 |
| CM vs. comb | BAL | 17 | 0 | 11 | 51 | 0.607 | 1.000 | 0.861 | 1.000 | 0.823 | 0.000 | 0.393 | 0.804 | 0.06 | 0.0000 |
| CM vs. PNP | BAL | 16 | 1 | 11 | 51 | 0.593 | 0.981 | 0.848 | 0.941 | 0.823 | 0.019 | 0.407 | 0.787 | 0.062 | 0.0000 |