Evaluation of the BioFire® FilmArray® Pneumonia plus Panel for Detecting Bacterial Etiological Agents of Lower Respiratory Tract Infections in an Oncologic Hospital. Comparison with Conventional Culture Method

1 Seventy-nine samples of BAL (bronchoalveolar lavage) were analyzed by CM and PNP. Twenty-seven samples were bacteriologically positive in the PNP test (reference method) at the detection level at > 10⁴ genomic copies/ml.

1* bacteria identified in CM, regardless of the result of the diagnostic titer; in the brackets number of cultures equal to or above the diagnostic threshold (> 10⁴–10⁵ CFU/ml (CFU – colony-forming units))

1** microorganisms not detected in the culture method

1*** microorganism not detected in the PNP test

1# ESBLs positive (extended-spectrum p-lactamases)

1 BAL – bronchoalveolar lavage, Comb = CM + PNP, TP – true positives, FP – false positives, FN – false negatives, TN – true negatives,

SENS – sensitivity, SP – specificity, ACC – accuracy, PPV – positive predictive value, NPV – negative predictive value, FPR – false positive ratio,

FNR – false negative ratio, AUC – area under the curve, SE – standard error of AUC, p – statistical significance level

The Spearman correlation coefficient R = 0.7466, p < 0.001

1 Sixty-nine samples were analyzed for fungi by CM.

The results are presented as CFU/ml (CFU – colony-forming units).

The diagnostic threshold was ≥ 10⁴ – 10⁵ CFU/ml. Fungi growth was obtained in 28 out of 69 samples (40.6%), but only in one sample was the growth of Candida in the diagnostic titer.