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Kestose Increases the Relative Abundance of Faecalibacterium spp. and Nominally Increases Cow Milk Tolerant Dose in Children with Cow's Milk Allergy – Preliminary Results Cover

Kestose Increases the Relative Abundance of Faecalibacterium spp. and Nominally Increases Cow Milk Tolerant Dose in Children with Cow's Milk Allergy – Preliminary Results

Polish Journal of Microbiology
Volume 72 (2023): Issue 3 (September 2023)
By:
Open Access
|Sep 2023

Figures & Tables

Fig. 1.

Changes in the patients’ immunological parameters.The laboratory data from 10 participants before and after the study are shown. a) Total Immunoglobulin E (tIgE), b) milk-specific Immunoglobulin E (sIgE), c) casein-specific Immunoglobulin G4 (IgG4), d) Thymus and Activation-Regulated Chemokine (TARC). No significant changes were seen after 12 weeks (p > 0.05, Wilcoxon's signed-rank test).
Changes in the patients’ immunological parameters.The laboratory data from 10 participants before and after the study are shown. a) Total Immunoglobulin E (tIgE), b) milk-specific Immunoglobulin E (sIgE), c) casein-specific Immunoglobulin G4 (IgG4), d) Thymus and Activation-Regulated Chemokine (TARC). No significant changes were seen after 12 weeks (p > 0.05, Wilcoxon's signed-rank test).

Fig. 2.

Relative abundance of gut microbiota at 0 and 12 weeks.This bar graph shows the composition of the intestinal microbiota in each of the 10 subjects. The numbers on the horizontal axis of the graphs represent the patient number, and the graphs in the same column represent the results for the same subject. The results for the top 17 genera with the highest occupancy are shown. The top row shows the results at the beginning of the study, and the bottom row shows the results 12 weeks into the study.
Relative abundance of gut microbiota at 0 and 12 weeks.This bar graph shows the composition of the intestinal microbiota in each of the 10 subjects. The numbers on the horizontal axis of the graphs represent the patient number, and the graphs in the same column represent the results for the same subject. The results for the top 17 genera with the highest occupancy are shown. The top row shows the results at the beginning of the study, and the bottom row shows the results 12 weeks into the study.

Fig. 3.

Absolute quantification of intestinal microbiota (qPCR).The logarithm of the genome copy number of Faecalibacterium prausnitzii per gram of feces is shown at baseline (0w) and after 12 weeks of intervention (12w). Dotted lines connect changes in the same participants. Eight of 10 patients exhibited nominal increases from 0 to 12 weeks, but this difference was not statistically significant (p > 0.05, Wilcoxon's signed-rank test).
Absolute quantification of intestinal microbiota (qPCR).The logarithm of the genome copy number of Faecalibacterium prausnitzii per gram of feces is shown at baseline (0w) and after 12 weeks of intervention (12w). Dotted lines connect changes in the same participants. Eight of 10 patients exhibited nominal increases from 0 to 12 weeks, but this difference was not statistically significant (p > 0.05, Wilcoxon's signed-rank test).

Relative abundance of gut microbiota_

0w12wp-value*
Median (25%–75%)Median (25%–75%)
xBifidobacterium16.2 (8.3–24.2)21.7 (13.3–24.1)0.72
Blautia9.5 (6.8–16.9)13.6 (10.0–22.7)0.24
Bacteroides4.0 (0.8–8.3)6.6 (3.7–9.8)0.20
Faecalibacterium3.8 (1.4–7.0)6.8 (4.4–9.7)0.01
Fusicatenibacter6.0 (0.3–8.1)5.3 (0.4–8.8)0.29
Anaerostipes5.0 (1.0–7.7)4.4 (2.3–7.3)0.39
Roseburia3.8 (0.5–5.3)0.8 (0.4–8.0)0.88
Lachnospiracea_incertae_sedis3.0 (2.7–5.3)3.8 (2.5–4.5)0.65
Gemmiger4.6 (1.0–7.5)4.7 (1.1–5.6)0.67
Streptococcus2.3 (0.9–6.5)2.4 (1.6–4.7)0.88
Clostridium XlVa2.9 (1.1–4.5)2.2 (1.8–3.9)0.24
Ruminococcus20.6 (0.4–3.1)0.8 (0.3–1.7)0.51
Veillonella1.8 (0.2–3.4)0.8 (0.2–3.2)0.96
Clostridium XI1.4 (0.1–3.0)0.6 (0.4–0.9)0.33
Dialister0.2 (0.0–2.2)0.9 (0.0–1.9)0.59
Butyricicoccus0.4 (0.1–1.3)0.9 (0.6–1.6)0.20
Clostridium IV0.6 (0.1–1.5)0.3 (0.2–1.0)0.45

Background information on the participants_

Number of participants10
Age (median/minimum-maximum)8/2–12
Male/female7/3
Atopic dermatitis8
Bronchial asthma7
Allergic rhinitis8
IgE (total) IU/ml (25%–75%)1,157 (384–2,744)
IgE (milk) kUA/l (25%–75%)50 (27–66)
EASI score (25%–75%)0.3 (0.05–2.1)
Serum TARC (pg/ml) (25%–75%)551 (333–976)

Oral food challenge results for all patients_

First OFCSecond OFC
TD (ml)TS (points)TS/ProTD (ml)TS (points)TS/Pro (points/g)
Patient 13.510948.5519
Patient 21.5255503.525236
Patient 31.7152913.719
Patient 41.7316010.720943
Patient 50.11033001.800
Patient 60.8166601.820367
Patient 73.7151343.715134
Patient 80.11033000.3252750
Patient 90.7115191.720388
Patient 103.7151343.740357
DOI: https://doi.org/10.33073/pjm-2023-030 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 299 - 306
Submitted on: Mar 24, 2023
Accepted on: Jun 30, 2023
Published on: Sep 20, 2023
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
cow's milk allergy,
gut microbiota,
Anaphylaxis Scoring Aichi (ASCA),
kestose
Related subjects:
Life sciences,
Microbiology and virology

© 2023 Shohei Kubota, Shiro Sugiura, Mayuko Takahashi, Yoshihiro Kadota, Yoshihiro Takasato, Teruaki Matsui, Katsumasa Kitamura, Takumi Tochio, Komei Ito, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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