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Contribution of YPRO15C Overexpression to the Resistance of Saccharomyces cerevisiae BY4742 Strain to Furfural Inhibitor Cover

Contribution of YPRO15C Overexpression to the Resistance of Saccharomyces cerevisiae BY4742 Strain to Furfural Inhibitor

Polish Journal of Microbiology
Volume 72 (2023): Issue 2 (June 2023)
By: Getachew Tafere Abrha,  Qian Li,  Xiaolin Kuang,  Difan Xiao,  Ellen Ayepa,  Jinjian Wu,  Huan Chen,  Zhengyue Zhang,  Yina Liu,  Xiumei Yu,  Quanju Xiang and  Menggen Ma  
Open Access
|Jun 2023

Figures & Tables

Construction illustration of the recombinant plasmid. a) pUG6-TEF1p-YPR015C, b) integration diagram of the recombinant plasmid pUG6-TEF1p-YPR into the chromosomal DNA of Saccharomyces cerevisiae.

Fig. 1.

Cell growth of Saccharomyces cerevisiae strains overexpressing YPR015C and BY4742 as measured at OD600 on a defined synthetic medium containing 35 mM of furfural. The prolonged lag phase indicates the inhibitory effect of furfural on the parental strain.

Fig. 2.

Furfural and HMF reduction of activities in the crude cell extracts of the parental (BY4742) and YPR015C overexpressing strains at 35 mM of furfural. The activities were measured using NADH or NADPH as cofactors. a) NADH and c) NADPH used for furfural reduction, b) and d) for HMF reduction, respectively. The data represent averages of three experiments. *p < 0.05; **p < 0.01 indicates significant differences.

Fig. 3.

Accumulation of reactive oxygen species (ROS) caused by furfural. a) Representative images of cells stained with the ROS indicator 2’,7’-dichlorofluorescein diacetate, b) percentage of cells at each concentration of furfural and peroxide that stained positive for ROS by 2’,7’-dichlorofluorescein diacetate after 2, 8, and 16 h. The data represent averages of three experiments with standard error. *p < 0.05; **p < 0.01 indicates significant differences when at least 100 cells were examined on each bright-field image.

Fig. 4.

Changes in cell densities after the treatment with lyticase during 4 h of incubation. Saccharomyces cerevisiae strains were treated for 2, 8, and 16 h (from top to bottom) with 35 mM of furfural, respectively. The mean values of relative optical density are presented with vertical error bars, each representing a single standard deviation (n = 3).
DOI: https://doi.org/10.33073/pjm-2023-019 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 177 - 186
Submitted on: Feb 9, 2023
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Accepted on: Apr 13, 2023
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Published on: Jun 14, 2023
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
furfural,
overexpressed,
tolerance,
transcriptomics
Related subjects:
Life sciences,
Microbiology and virology

© 2023 Getachew Tafere Abrha, Qian Li, Xiaolin Kuang, Difan Xiao, Ellen Ayepa, Jinjian Wu, Huan Chen, Zhengyue Zhang, Yina Liu, Xiumei Yu, Quanju Xiang, Menggen Ma, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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