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Ciliated Peritrichous Protozoa in a Tezontle-Packed Sequencing Batch Reactor as Potential Indicators of Water Quality Cover

Ciliated Peritrichous Protozoa in a Tezontle-Packed Sequencing Batch Reactor as Potential Indicators of Water Quality

Polish Journal of Microbiology
Volume 71 (2022): Issue 4 (December 2022)
By: Andrea M. Rivas-Castillo,  Antonio A. Garcia-Barrera,  Aristeo Garrido-Hernandez,  Francisco J. Martinez-Valdez,  Maria S. Cruz-Romero and  Maribel Quezada-Cruz  
Open Access
|Dec 2022

Figures & Tables

Fig. 1

Micrographs of colonial peritrichous protozoa. a) Fresh sample and b) stained with Harris hematoxylin technique at 40x; c) 4-zoid fresh colony using Nomarski technique and d) FD staining at 40x; e) 8-zoid fresh colony using Nomarski technique and f) the MFD staining at 40x; and g) fresh samples of Vorticella campanula and h) stained with the MFD technique (“J” shaped nucleus) at 20x magnification.

Fig. 2

Identification of Vorticella campanula 01mex by a phylogenetic analysis based on its ITS 18S rRNA sequence.

Fig. 3.

Relationship between protozoan density and COD in the SBR. Protozoa density; Influent COD; Effluent COD; Removal efficiency (%).

Fig. 4

Behavior of sessile ciliates with respect to the concentration of total solids.

Fig. 5

Principal Component Analysis (PCA): relationship between the abundance of protozoa and other microorganisms, time, and removal percentage. Axes 1 and 2 account for 54.9% and 22.1% of the total variation presented, respectively.

Fig. 6

Properties of tezontle as supporting material of the SBR. a) A piece of tezontle containing adhered peritrichous colonies; b) Scanning Electron Microscopy (SEM) of tezontle stones; c) closeup of tezontle pores.

The density of peritrichous protozoa (peritrichous protozoa × 103/ml)_

Time (days)Zoothamnium paraentziiEpistylis plicatilisVorticella campanulaEpistylis rotansCarchesium polypinumVorticella convallariaVorticella aquadulcisTotal (× 103density protozoa/ml)
1118107331.5172
101423457471722299
202673457471722425
302209296761152500
402446376611432462
602327786681242481

The mean abundance of peritrichous protozoan species_

SpeciesAverage density (protozoa × 103/ml)
Zoothamnium paraentzii185.79a
Epistylis plicatilis  51.18b
Vorticella campanula  63.56b
Epistylis rotans  50.79b
Carchesium polypinum17.31c
Vorticella convallaria   2.85d
Vorticella aquadulcis1.64d
DOI: https://doi.org/10.33073/pjm-2022-049 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 539 - 551
Submitted on: Jun 29, 2022
|
Accepted on: Oct 2, 2022
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Published on: Dec 10, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
peritrichous protozoa,
sessile ciliates,
Sequencing Batch Reactor (SBR),
tezontle,
protozoan staining techniques
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Andrea M. Rivas-Castillo, Antonio A. Garcia-Barrera, Aristeo Garrido-Hernandez, Francisco J. Martinez-Valdez, Maria S. Cruz-Romero, Maribel Quezada-Cruz, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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