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The Emergence of Colistin- and Imipenem-Associated Multidrug Resistance in Escherichia coli Isolates from Retail Meat Cover

The Emergence of Colistin- and Imipenem-Associated Multidrug Resistance in Escherichia coli Isolates from Retail Meat

Polish Journal of Microbiology
Volume 71 (2022): Issue 4 (December 2022)
By: Benson Chuks Iweriebor,  Olivia Sochi Egbule and  Larry C. Obi  
Open Access
|Dec 2022

Figures & Tables

Fig. 1

Agarose gel electrophoresis for Escherichia coli identification. Line M – 100 bp ladder, Line 1 – negative control, Line 2 – E. coli ATCC® 25922™ as a positive control, Lines 3–12 – the 147 bp PCR amplification product for E. coli isolates.
Agarose gel electrophoresis for Escherichia coli identification. Line M – 100 bp ladder, Line 1 – negative control, Line 2 – E. coli ATCC® 25922™ as a positive control, Lines 3–12 – the 147 bp PCR amplification product for E. coli isolates.

Fig. 2

The sensitivity pattern of Escherichia coli isolates against antibiotics. AMP – ampicillin, CO10 – colistin sulphate, TS – trimethoprim-sulphamethoxazole, CXM – cefuroxime, E – erythromycin, C30 – chloramphenicol, IMI – imipenem, GM – gentamycin, T– tetracycline, NOR – norfloxacin, CTX – cefotaxime, CIP – ciprofloxacin, S – susceptible, R – resistant, I – intermediate
The sensitivity pattern of Escherichia coli isolates against antibiotics. AMP – ampicillin, CO10 – colistin sulphate, TS – trimethoprim-sulphamethoxazole, CXM – cefuroxime, E – erythromycin, C30 – chloramphenicol, IMI – imipenem, GM – gentamycin, T– tetracycline, NOR – norfloxacin, CTX – cefotaxime, CIP – ciprofloxacin, S – susceptible, R – resistant, I – intermediate

Fig. 3

Electrophoresis of mrc-1 gene amplification among Escherichia coli isolates. Line 1 – 100 bp ladder, Line 2 – negative control, Line 3–13 – positive isolates
Electrophoresis of mrc-1 gene amplification among Escherichia coli isolates. Line 1 – 100 bp ladder, Line 2 – negative control, Line 3–13 – positive isolates

Fig. 4

Electrophoresis of the ermB gene amplification product (350 bp) in Escherichia coli isolates. Line 1 – 100 bp DNA ladder, Line 2 – negative control, Lines 3–12 – the ermB-resistant E. coli isolates
Electrophoresis of the ermB gene amplification product (350 bp) in Escherichia coli isolates. Line 1 – 100 bp DNA ladder, Line 2 – negative control, Lines 3–12 – the ermB-resistant E. coli isolates

Fig. 5

Gel electrophoresis of the PCR product (690 bp) amplified with blaTEM primers for the detection of β-lactam-resistant Escherichia coli isolates. Line 1 – DNA ladder, Line 2 – negative control, Lines 3–9 – β-lactam-resistant E. coli isolates
Gel electrophoresis of the PCR product (690 bp) amplified with blaTEM primers for the detection of β-lactam-resistant Escherichia coli isolates. Line 1 – DNA ladder, Line 2 – negative control, Lines 3–9 – β-lactam-resistant E. coli isolates

Isolation and identification of Escherichia coli_

ShopLocation of shopSamples collectedPresumptive isolatesPositive isolates%
AOpen-air market58582848.28
BOpen-air market33311858.06
CClosed store4343818.60
DClosed store42421433.33
Total 1761746839.08

Antibiotic resistance patterns and the Multiple Antibiotic Resistance Index (MARI) of the confirmed E_ coli isolates_

MDR phenotypeNumber of antimicrobialsMARI
CTX-T-E30.25
CTX-E-C3030.25
CTX-AMP-E30.25
CTX-CXM-E30.25
TS-GM-E-CO1040.33
CTX-CXM-AMP-E40.33
AMP-T-TS-C30-E50.42
CTX-CXM-T-TS-E50.42
CTX-CXM-AMP-TS-E50.42
CTX-CXM-AMP-T-E50.42
CTX-CXM-TS-CO10-E50.42
CTX-TS-E-IMI-CIP50.42
CTX-CXM-AMP-T-E-C3060.50
CTX-CXM-AMP-TS-CO10-E60.50
CTX-CXM-AMP-T-TS-E60.50
CTX-CXM-AMP-TS-IMI-E60.50
CTX-CXM-AMP-IMI-CO10-E60.50
CTX-CXM-AMP-TS-IMI-E60.50
CTX-CXM-AMP-TS-C30-GM60.50
CTX-CXM-AMP-T-TS-C30-E70.58
CTX-CXM-AMP-TS-IMI-E60.50
CTX-CXM-AMP-T-TS-C30-IMI-E80.66
CTX-CXM-AMP-T-TS-C30-CO10-E80.66
CTX-CXM-AMP-T-TS-C30-GM-CO1080.66
CTX-AMP-T-TS-E-C30-IMI-NOR80.66
CTX-CXM-AMP-T-TS-C30-IMI-CO10-E90.75
CTX-CXM-AMP-TS-C30-IMI-GM-CO10-E90.75

Colistin and imipenem resistance patterns among the isolates_

Colistin- and imipenem-resistant isolates (CO10+/IMP+)Colistin-resistant and imipenem-sensitive isolates (CO10+/IMP–)Colistin-sensitive and imipenem-resistant isolates (CO10–/IMP+)
CTX-CXM-AMP-TS-C30-IMI-GM-CO10-ECTX-CXM-AMP-T-TS-C30-GM-CO10CTX-AMP-T-TS-E-C30-IMI-NOR
CTX-CXM-AMP-T-TS-C30-IMI-CO10-ECTX-CXM-AMP-T-TS-C30-CO10-ECTX-CXM-AMP-T-TS-E-C30-IMI-E
CTX-CXM-AMP-IMI-CO10-ETS-GM-E-CO10CTX-CXM-AMP-TS-IMI-E
CTX-CXM-TS-CO10-ECTX-CXM-AMP-TS-E
CTX-CXM-AMP-TS-CO10-ECTX-TS-E-IMI-CIP
DOI: https://doi.org/10.33073/pjm-2022-046 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 519 - 528
Submitted on: Jul 15, 2022
|
Accepted on: Sep 19, 2022
|
Published on: Dec 6, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
pork,
multiple antibiotic resistance,
susceptibility,
retail outlet,
colistin resistance,
imipenem resistance
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Benson Chuks Iweriebor, Olivia Sochi Egbule, Larry C. Obi, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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