Fig. 1
Line chart of alpha diversity index for different sampling times; a) dynamic changes of Chao1 index, b) dynamic changes of Shannon index. The different letters indicate the significant difference at the 0.05 level (p < 0.05, n = 5).
Table I
The non-parametric Wilcox test results.
| The non-parametric Wilcox test results of Chao1 index | |||||
|---|---|---|---|---|---|
| Difference | p-value | sig. | LCL | UCL | |
| HE1-IN1 | –4.6 | 0.3909 | –15.3189 | 6.118942 | |
| HE2-IN2 | 1.2 | 0.8221 | –9.51894 | 11.91894 | |
| HE3-IN3 | –8.6 | 0.1128 | –19.3189 | 2.118942 | |
| HE4-IN4 | –9.8 | 0.072 | –20.5189 | 0.918942 | |
| HE5-IN5 | –20 | 0.0005 | *** | –30.7189 | –9.28106 |
| The non-parametric Wilcox test results of Shannon index | |||||
| Difference | p-value | sig. | LCL | UCL | |
| HE1-IN1 | –6.8 | 0.1353 | –15.8168 | 2.216805 | |
| HE2-IN2 | –17.8 | 0.0003 | *** | –26.8168 | –8.78319 |
| HE3-IN3 | –26.2 | 0 | *** | –35.2168 | –17.1832 |
| HE4-IN4 | –12.4 | 0.0083 | ** | –21.4168 | –3.38319 |
| HE5-IN5 | –8.2 | 0.0735 | –17.2168 | 0.816805 | |
Fig. 2
Column map of species relative abundance at phylum level (Group Analysis).
Fig. 3
The group’s phylogenetic relationship of genus species at the level of analysis. A phylogenetic tree constructed from representative sequences of genus levels. The color of the branches and sectors represents the corresponding phylum. The stacked column diagram outside the fan ring illustrates the abundance distribution information of the genus in different samples.
Fig. 4
Species relative abundance of species at the genus level (by group analysis); a) relative abundance of the top 10 genera, b) relative abundance of the top 30 genera.
Fig. 5
Heat map of the Genus-level species abundance clustering (by group analysis). The longitudinal direction is the sample information, and the horizontal direction is the species annotation information. The cluster tree on the left side of figure is the species clustering tree. Different colors represent different relative abundances, red represents the high relative abundance, and blue represents the low relative abundance.
Fig. 6
Line chart of relative abundance for different samples; a) Relative abundance of Colletotrichum spp. at different sampling times, b) relative abundance of Fusarium spp. at different sampling times, c) relative abundance of Alternaria spp. at different sampling times, d) relative abundance of Colletotrichum spp., Fusarium spp., and Alternaria spp. The different letters indicate the significant difference at the 0.05 level (p < 0.05, n = 5).
Fig. 7
Phyllosphere fungal assemblage dissimilarity among healthy (HE1 ~ 5) and infected (IN1 ~ 5) leaves, represented by nonmetric multidimensional scaling (NMDS).