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Structural and Dynamic Analysis of Leaf-Associated Fungal Community of Walnut Leaves Infected by Leaf Spot Disease Based Illumina High-Throughput Sequencing Technology Cover

Structural and Dynamic Analysis of Leaf-Associated Fungal Community of Walnut Leaves Infected by Leaf Spot Disease Based Illumina High-Throughput Sequencing Technology

Polish Journal of Microbiology
Volume 71 (2022): Issue 3 (September 2022)
By: Shiwei Wang,  Yu Tan,  Shujiang Li and  Tianhui Zhu  
Open Access
|Sep 2022

Figures & Tables

Fig. 1

Line chart of alpha diversity index for different sampling times; a) dynamic changes of Chao1 index, b) dynamic changes of Shannon index. The different letters indicate the significant difference at the 0.05 level (p < 0.05, n = 5).
Line chart of alpha diversity index for different sampling times; a) dynamic changes of Chao1 index, b) dynamic changes of Shannon index. The different letters indicate the significant difference at the 0.05 level (p < 0.05, n = 5).

Fig. 2

Column map of species relative abundance at phylum level (Group Analysis).
Column map of species relative abundance at phylum level (Group Analysis).

Fig. 3

The group’s phylogenetic relationship of genus species at the level of analysis. A phylogenetic tree constructed from representative sequences of genus levels. The color of the branches and sectors represents the corresponding phylum. The stacked column diagram outside the fan ring illustrates the abundance distribution information of the genus in different samples.
The group’s phylogenetic relationship of genus species at the level of analysis. A phylogenetic tree constructed from representative sequences of genus levels. The color of the branches and sectors represents the corresponding phylum. The stacked column diagram outside the fan ring illustrates the abundance distribution information of the genus in different samples.

Fig. 4

Species relative abundance of species at the genus level (by group analysis); a) relative abundance of the top 10 genera, b) relative abundance of the top 30 genera.
Species relative abundance of species at the genus level (by group analysis); a) relative abundance of the top 10 genera, b) relative abundance of the top 30 genera.

Fig. 5

Heat map of the Genus-level species abundance clustering (by group analysis). The longitudinal direction is the sample information, and the horizontal direction is the species annotation information. The cluster tree on the left side of figure is the species clustering tree. Different colors represent different relative abundances, red represents the high relative abundance, and blue represents the low relative abundance.
Heat map of the Genus-level species abundance clustering (by group analysis). The longitudinal direction is the sample information, and the horizontal direction is the species annotation information. The cluster tree on the left side of figure is the species clustering tree. Different colors represent different relative abundances, red represents the high relative abundance, and blue represents the low relative abundance.

Fig. 6

Line chart of relative abundance for different samples; a) Relative abundance of Colletotrichum spp. at different sampling times, b) relative abundance of Fusarium spp. at different sampling times, c) relative abundance of Alternaria spp. at different sampling times, d) relative abundance of Colletotrichum spp., Fusarium spp., and Alternaria spp. The different letters indicate the significant difference at the 0.05 level (p < 0.05, n = 5).
Line chart of relative abundance for different samples; a) Relative abundance of Colletotrichum spp. at different sampling times, b) relative abundance of Fusarium spp. at different sampling times, c) relative abundance of Alternaria spp. at different sampling times, d) relative abundance of Colletotrichum spp., Fusarium spp., and Alternaria spp. The different letters indicate the significant difference at the 0.05 level (p < 0.05, n = 5).

Fig. 7

Phyllosphere fungal assemblage dissimilarity among healthy (HE1 ~ 5) and infected (IN1 ~ 5) leaves, represented by nonmetric multidimensional scaling (NMDS).
Phyllosphere fungal assemblage dissimilarity among healthy (HE1 ~ 5) and infected (IN1 ~ 5) leaves, represented by nonmetric multidimensional scaling (NMDS).

The non-parametric Wilcox test results_

The non-parametric Wilcox test results of Chao1 index
Differencep-valuesig.LCLUCL
HE1-IN1  –4.60.3909 –15.3189  6.118942
HE2-IN2   1.20.8221 –9.5189411.91894
HE3-IN3  –8.60.1128 –19.3189  2.118942
HE4-IN4  –9.80.072 –20.5189  0.918942
HE5-IN5–200.0005***–30.7189–9.28106
The non-parametric Wilcox test results of Shannon index
Differencep-valuesig.LCLUCL
HE1-IN1  –6.80.1353 –15.8168  2.216805
HE2-IN2–17.80.0003***–26.8168–8.78319
HE3-IN3–26.20***–35.2168–17.1832
HE4-IN4–12.40.0083**–21.4168–3.38319
HE5-IN5  –8.20.0735 –17.2168  0.816805
DOI: https://doi.org/10.33073/pjm-2022-038 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 429 - 441
Submitted on: Apr 1, 2022
Accepted on: Jul 25, 2022
Published on: Sep 24, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
dynamic analysis,
high throughput sequencing,
leaf-associated fungi,
spot disease,
walnut
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Shiwei Wang, Yu Tan, Shujiang Li, Tianhui Zhu, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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