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Genome-Guided Investigation Provides New Insights into Secondary Metabolites of Streptomyces parvulus SX6 from Aegiceras corniculatum Cover

Genome-Guided Investigation Provides New Insights into Secondary Metabolites of Streptomyces parvulus SX6 from Aegiceras corniculatum

Polish Journal of Microbiology
Volume 71 (2022): Issue 3 (September 2022)
By: ,  ,  ,  ,  ,  ,  ,   and    
Open Access
|Sep 2022
Figures & tables

Figures & Tables

Fig. 1

Heatmap presenting antibacterial activity against at least one tested pathogenic bacteria of endophytic actinobacteria isolated from Aegiceras corniculatum. BC – Bacillus cereus ATCC® 11778™, PA – Pseudomonas aeruginosa ATCC® 9027™, MRSE – methicillin-resistant Staphylococcus epidermidis ATCC® 35984™, EA – Enterobacter aerogenes ATCC® 13048™, EC – Escherichia coli ATCC® 11105™, ST – Salmonella typhimurium ATCC® 14028™.

Fig. 2

Identification of endophytic strain SX6. A) Scanning electron microscopy of hyphae of strain SX6 grown on ISP2 medium; B) phylogenetic tree based on 16S rRNA gene sequences of strain SX6 and closely related Streptomyces strains.

Fig. 3

Biological activities determined in the Streptomyces parvulus SX6 extract. A) Selective antibacterial activity against MRSE ATCC® 35984™ and Pseudomonas aeruginosa ATCC® 9027™; B) cytotoxic activity against A549, Hep3B, MCF-7, and HEK-293 cell lines; C) and D) antioxidant activities, including DPPH free radical scavenging and hydroxyl radical scavenging were determined in the SX6 extract. Mean values and SD of three independent experiments are shown and p-values were calculated by the Student’s unpaired two-tailed t-test by the graph prism software (nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001).

Fig. 4

Genome characterization of Streptomyces parvulus SX6. A) Clusters of Orthologous Groups (COGs) of protein functions; B) whole genome-based phylogenetic classification of S. parvulus SX6. The numbers on the branches are GBDP pseudo-bootstrap support values of > 60% from 100 replications, with average branch support of 96.6%.

Fig. 5

Cryptic secondary metabolite biosynthetic gene clusters identified in the genome of Streptomyces parvulus SX6.

Fig. 6

Biosynthetic pathway of daidzein and genistein in Streptomyces parvulus SX6. A) Using genome mining, the proposed biosynthetic pathways of daidzein and genistein present in S. parvulus SX6; B) identification of genes involved in daidzein and genistein biosynthesis across S. parvulus genomes; C) HPLC-DAD-MS chromatogram of reference compounds and the extract of mangrove endophytic S. parvulus SX6.

Features of the SX6 and other Streptomyces parvulus genomes_

SpeciesSize (Mb)GC (%)GenesCDSstRNAsrRNAs
S. parvulus SX67.6972.86,9526,7796811
S. parvulus JCM 40687.6971.56,8816,866663
S. parvulus 22977.1572.76,9516,7736618
S. parvulus LP037.7472.57,0306,832663
DOI: https://doi.org/10.33073/pjm-2022-034 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 381 - 394
Submitted on: Mar 16, 2022
Accepted on: Jul 13, 2022
Published on: Sep 24, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Aegiceras corniculatum,
Streptomyces parvulus,
genome mining,
plant-derived compounds,
secondary metabolites
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Ngoc Tung Quach, Thi Hanh Nguyen Vu, Thi Lien Bui, Anh Tuan Pham, Thi Thu An Nguyen, Thi Thanh Xuan Le, Thi Thu Thuy Ta, Pravin Dudhagara, Quyet-Tien Phi, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 71 (2022): Issue 3 (September 2022)
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