Nutrients Changed the Assembly Processes of Profuse and Rare Microbial Communities in Coals

Yuanyuan Zhang; Sheng Xue; Xiaohua Chang; Yang Li; Xuelian Yue

doi:10.33073/pjm-2022-032

Figures & Tables

a) Chao1 index and b) Shannon index of the abundant and rare genera in coals. The Wilcoxon test was used to compare the differences in microbial diversities.

a) Ordering of the abundant community compositions at the genus level by nonmetric multidimensional scaling (NMDS) based on the Bray-Curtis dissimilarity index; b) ordering of the rare community compositions at the genus level by NMDS based on the Bray-Curtis distance index; c) difference for Bray-Curtis dissimilarities among nutrient and ck groups. The Wilcoxon test was used to compare the differences in Bray-Curtis dissimilarities.

Manhattan plot of the changes in a) abundant and b) rare genera in coals under nutrient stimulation. Detailed information is shown in Tables SII and SIII.

Heatmap for a) the profuse and b) rare known genera (detected in more than 30% of samples).

Redundancy analysis (RDA) for coal characteristics (including TC, TN, TO, TH, Vdaf, Ad, and Mad) on the coal microbial compositions. a) the effect of coal characteristics on profuse microbial compositions in the ck group; b) the effect of coal characteristics on rare microbial compositions in the ck group; c) the effect of coal characteristics on profuse microbial compositions in the nutrient group; d) the effect of coal characteristics on rare microbial compositions in the nutrient group; e) the effect of nutrients on profuse microbial compositions; f) the effect of nutrients on rare microbial compositions.

Deterministic and stochastic processes in community assembly. a) βNTI index in abundant and rare groups; b) the relative influences of deterministic and stochastic assembly processes in shaping abundant and rare groups. Detailed information is shown in Tables SIV–SVII.

Co-occurrence networks of abundant and rare groups in coals based on the correlation analysis. Detailed information is shown in Tables SVIII–SXI. Pro – Proteobacteria, Act – Actinobacteria, Bact – Bacteroidetes, Fir – Firmicutes, Ver – Verrucomicrobia, Pla – Planctomycetes, Spi – Spirochaetes, Acido – Acidobacteria, Eury – Euryarchaeota, Cren – Crenarchaeota, Gem – Gemmatimonadetes, Chl – Chloroflexi, and Syn – Synergistetes.

Detailed sample information on 59 microbial communities in coals for meta-analysis_

ID/NCBI accession number	Sites	Primer sequences	Coal rank	Treatment	References
SRR9312778-SRR9312784	Erlian Basin	515F: GTGCCAGCMGCCGCGG907R: CCGTCAATTCMTTTRAGTTT	Lignite	ck (no treat	(Wang et al. 2019a)
SRR6998887	Moghla	515F: GTGCCAGCMGCCGCGGTAA806R: GGACTACHVGGGTWTCTAAT	Bituminous		(Sharma et al. 2019)
SRR1695964	Queensland	926F: AAACTYAAAKGAATTGACGG1392R: ACGGGCGGTGTGTRC	Bituminous		(Raudsepp et al. 2016)
SRR1695967
SRR1695969
SRR1695971
SRR5342611	Powder River Basin	341F: CCTACGGGNBGCASCAG805R: GACTACNVGGGTATCTAATCC	Bituminous		(Davis et al. 2018)
SRR8373697-SRR8373705	Anhui	338F: ACTCCTACGGGAGGCAGCAG806R: GGACTACHVGGGTWTCTAAT	Bituminous		(Liu et al. 2019)
SRR8373722-SRR8373724	Anhui		Bituminous
SRR8373719-SRR8373721	Guizhou		Anthracite
SRR8373696	Shanxi		Bituminous
SRR8373718
SRR8373734-SRR8373745
SRR8373746			Anthracite
SRR8373747			Anthracite
SRR7271165	Huaibei Coalfield	515F: GTGCCAGCMGCCGCGG907R: CCGTCAATTCMTTTRAGTTT	Bituminous	nutrients	(Wang et al. 2019b)
SRR11128868	Konin Basin	341F: CCTACGGGNGGCWGCAG785R: GACTACHVGGGTATCTAATCC	Lignite		(Bucha et al. 2020)
SRR5826886	New South Wales		Bituminous		(in 't Zandt et al. 2018)
SRR5826888
SRR5826889
SRR11241403	Upper Coal Basin Silesian		Bituminous		(Pytlak et al. 2020)
SRR5397976	Konin Basin	314F: CCTACGGGNGGCWGCAG805R: GACTACHCGGGTATCTAATCC	Bituminous		(Detman et al. 2018)
SRR7422168	Jiaozuo		Anthracite		(Su et al. 2018)
SRR7422169	Neimeng		Bituminous
SRR7422170	Suzhou		Bituminous
SRR7422171	Jingcheng		Anthracite
SRR7422172	Hebi		Bituminous
SRR7422173	Shaqu		Bituminous
SRR7422174	Liyazhuang		Bituminous
SRR7422175	Yima		Bituminous
SRR7422176	Pingdingshan		Bituminous
SRR7422177	Shoushan		Bituminous
SRR5342597	Powder River Basin	341F: CCTACGGGNBGCASCAG805R: GACTACNVGGGTATCTAATCC	Bituminous		(Davis et al. 2018)
SRR5342605		341F: CCTACGGGNBGCASCAG805R: GACTACNVGGGTATCTAATCC	Bituminous		(Davis et al. 2018)

Nutrients Changed the Assembly Processes of Profuse and Rare Microbial Communities in Coals

Figures & Tables

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Detailed sample information on 59 microbial communities in coals for meta-analysis_

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