Table I
The composition of neutralizers used in the tests.
| Disinfectants/reference substances | Composition |
|---|---|
| A) Glutardialdehyde | polysorbate 80 – 30 g/l |
| saponin – 30 g/l | |
| histidine – 1 g/l in diluent | |
| B) Peracetic acid | polysorbate 80 – 10 g/l |
| sodium thiosulfate – 5 g/l | |
| lecithin – 3 g/l in diluent |
Table II
The susceptibility of Clostridium sporogenes ATCC 3584 spores to glutardialdehyde and peracetic acid under the parameters defined for Clostridioides difficile R027 spores (results from two independent experiments, the reproducibility of PN-EN 13704: 2018-09 method was ± 0.09).
| Reference substance | Concentration [%] | Contact time [min] | The reduction required [lg] | The reduction obtained [lg] |
|---|---|---|---|---|
| Glutardialdehyde | 1.0 | 15 | < 1.5 | < 1.82 |
| Glutardialdehyde | 6.0 | 15 | ≥ 1.5 | 2.11 |
| Peracetic acid | 0.01 | 15 | < 1.5 | 2.54 |
| Peracetic acid | 0.04 | 15 | ≥ 1.5 | > 3.38 |
Table III
Sporicidal activity of the glutaraldehyde-based disinfectant A. Composition: 2.5% glutardialdehyde (results from three independent experiments, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0.09).
| Disinfectant | Concentration [%] | Contact time [min] | Organic load conditions | The reduction required [lg] | The reduction obtained [lg] |
|---|---|---|---|---|---|
| A | 80 | 15 | clean* | ≥ 3 | < 1.84 |
| A | 80 | 60 | clean | ≥ 3 | > 3.19 |
| A | 80 | 60 | dirty** | ≥ 3 | 3.24 |
Table IV
Sporicidal activity of peracetic acid-based disinfectant B. Composition: 1% product solution contains 0.15% peracetic acid (results from 1 experiment, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0.09).
| Disinfectant | Concentration [%] | Contact time [min] | Organic load conditions | The reduction required [lg] | The reduction obtained [lg] |
|---|---|---|---|---|---|
| B | 1 | 15 | clean* | ≥ 3 | > 3.31 |
| B | 1 | 15 | dirty** | ≥ 3 | > 3.31 |