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Susceptibility of Clostridium sporogenes Spores to Selected Reference Substances and Disinfectants Cover

Susceptibility of Clostridium sporogenes Spores to Selected Reference Substances and Disinfectants

Open Access
|Sep 2022

Figures & Tables

Sporicidal activity of peracetic acid-based disinfectant B_ Composition: 1% product solution contains 0_15% peracetic acid (results from 1 experiment, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0_09)_

DisinfectantConcentration [%]Contact time [min]Organic load conditionsThe reduction required [lg]The reduction obtained [lg]
B115clean*≥ 3> 3.31
B115dirty**≥ 3> 3.31

The susceptibility of Clostridium sporogenes ATCC 3584 spores to glutardialdehyde and peracetic acid under the parameters defined for Clostridioides difficile R027 spores (results from two independent experiments, the reproducibility of PN-EN 13704: 2018-09 method was ± 0_09)_

Reference substanceConcentration [%]Contact time [min]The reduction required [lg]The reduction obtained [lg]
Glutardialdehyde1.015< 1.5< 1.82
Glutardialdehyde6.015≥ 1.52.11
Peracetic acid0.0115< 1.52.54
Peracetic acid0.0415≥ 1.5> 3.38

Sporicidal activity of the glutaraldehyde-based disinfectant A_ Composition: 2_5% glutardialdehyde (results from three independent experiments, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0_09)_

DisinfectantConcentration [%]Contact time [min]Organic load conditionsThe reduction required [lg]The reduction obtained [lg]
A8015clean*≥ 3< 1.84
A8060clean≥ 3> 3.19
A8060dirty**≥ 33.24

The composition of neutralizers used in the tests_

Disinfectants/reference substancesComposition
A) Glutardialdehydepolysorbate 80 – 30 g/l
saponin – 30 g/l
histidine – 1 g/l in diluent

B) Peracetic acidpolysorbate 80 – 10 g/l
sodium thiosulfate – 5 g/l
lecithin – 3 g/l in diluent
DOI: https://doi.org/10.33073/pjm-2022-031 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 353 - 358
Submitted on: Mar 22, 2022
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Accepted on: Jul 6, 2022
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Published on: Sep 24, 2022
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2022 Agnieszka Chojecka, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.