Have a personal or library account? Click to login
A Novel Bioflocculant Produced by Cobetia marina MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae Cover

A Novel Bioflocculant Produced by Cobetia marina MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae

Polish Journal of Microbiology
Volume 71 (2022): Issue 3 (September 2022)
By: Siyu Zeng,  Yinghua Lu,  Xueshan Pan and  Xueping Ling  
Open Access
|Sep 2022

Figures & Tables

Fig. 1

Temporal courses of the a) bioflocculant, b) yield of biomass, and c) consumption of glucose, under different initial levels of glucose.
Temporal courses of the a) bioflocculant, b) yield of biomass, and c) consumption of glucose, under different initial levels of glucose.

Fig. 2

a) Effects of the N source alone on the biomass and bioflocculant production, b) effect of the ratio of combined N source on the biomass and bioflocculant production. A/YE – yeast extract, B/Glu – glutamate, C – tryptone, D – ammonium nitrate, E – ammonia chloride, F – urea. The control group was cultured in a minimal medium.
a) Effects of the N source alone on the biomass and bioflocculant production, b) effect of the ratio of combined N source on the biomass and bioflocculant production. A/YE – yeast extract, B/Glu – glutamate, C – tryptone, D – ammonium nitrate, E – ammonia chloride, F – urea. The control group was cultured in a minimal medium.

Fig. 3

The change curve of pH for the a) biomass and b) bioflocculant.
The change curve of pH for the a) biomass and b) bioflocculant.

Fig. 4

RSM and contour plots illustrating the interplaying effects of various factors on bioflocculant production; a–b) glucose (g/l) and yeast extract (g/l), c–d) glucose (g/l) and glutamate (g/l), e–f) yeast extract (g/l) and glutamate (g/l).
RSM and contour plots illustrating the interplaying effects of various factors on bioflocculant production; a–b) glucose (g/l) and yeast extract (g/l), c–d) glucose (g/l) and glutamate (g/l), e–f) yeast extract (g/l) and glutamate (g/l).

Fig. 5

Temporal courses of cellular growth, bioflocculant production, and pH for C. marina MCCC1113 cultivated in the optimal medium inside a rotary shaker at 150 rpm and 28°C for 72 h.
Temporal courses of cellular growth, bioflocculant production, and pH for C. marina MCCC1113 cultivated in the optimal medium inside a rotary shaker at 150 rpm and 28°C for 72 h.

Flocculation efficiency of bioflocculant produced by C_ marina on various microalgae_

Flocculation time (min)GroupH. pluvialisC. vulgarisS. platensis
FE (%)
5Treatment32.1–69.4
Control26.4–32.9

Effect analysis of independent variables in Plackett-Burman design_

FactorLevelpSignificant
–11
Glucose25350.0022++
Yeast extract5150.004++
Glutamate130.025+
Culture time28360.85–
pH780.861–

ANOVA for response surface quadratic model_

SourceSum of squareDFMean squareF-valueProb > F
Model0.76  90.0847.020.0088
Residual  0.084  70.012
Lack of fit  0.039  30.0131.160.4282
Pure error  0.045  4
Cor total0.8416
R2 = 0.9803R2adj = 0.7721R2pred = 0.1744CV = 13.53Adeq. precision = 7.526

Determination of the optimum ratio of bioflocculant/microalgae (v/v)_

FE (%) of different algaeAmount of BF/algae added (%)
05101520
H. pluvialis2034.836.747.263
S. platensis32.986.487.889.493.9
C. vulgaris29.148.153.253.151.1

Box-Behnken design arrangement and responses_

RUNGlucoseYeast extractGlutamateBioflocculant
(g/l)(g/l)(g/l)(g/l)
1301021.30
2301021.19
330510.9
435520.65
5301530.95
6301020.89
7351030.79
8251520.57
930530.87
10251010.32
1125520.54
12351010.79
13301021.22
14301021.28
15301510.92
16251030.58
17351520.65
DOI: https://doi.org/10.33073/pjm-2022-030 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Journal RSS Feed
Language: English
Page range: 341 - 351
Submitted on: Mar 14, 2022
Accepted on: Jul 3, 2022
Published on: Sep 24, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
MCCC1113,
medium optimization,
response surface methodology,
bioflocculant,
microalgae
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Siyu Zeng, Yinghua Lu, Xueshan Pan, Xueping Ling, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Previous articleVolume 71 (2022): Issue 3 (September 2022)Next article