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Role of MicroRNA-Like RNAs in the Regulation of Spore Morphological Differences in the Entomopathogenic Fungus Metarhizium acridum Cover

Role of MicroRNA-Like RNAs in the Regulation of Spore Morphological Differences in the Entomopathogenic Fungus Metarhizium acridum

Polish Journal of Microbiology
Volume 71 (2022): Issue 3 (September 2022)
By: Erhao Zhang,  Jie Zhang,  Rundong Zhao,  Yazhou Lu,  Xiu Yin,  Xiaozhong Lan and  Zhang Luo  
Open Access
|Sep 2022

Figures & Tables

Fig. 1

Overview of mRNA expression profiles and DEGs in BS and CO.A) The gene expression distribution. B) The DEGs in BS and CO, significantly downregulated genes, and upregulated genes are identified with |log2FC| ≥ 1 and p-values ≤ 0.05.
Overview of mRNA expression profiles and DEGs in BS and CO.A) The gene expression distribution. B) The DEGs in BS and CO, significantly downregulated genes, and upregulated genes are identified with |log2FC| ≥ 1 and p-values ≤ 0.05.

Fig. 1

Overview of mRNA expression profiles and DEGs in BS and CO.C) Heatmap of Pearson correlations of the expression levels among samples.
Overview of mRNA expression profiles and DEGs in BS and CO.C) Heatmap of Pearson correlations of the expression levels among samples.

Fig. 2

Functional analysis of the DEGs.A) Gene ontology analysis.
Functional analysis of the DEGs.A) Gene ontology analysis.

Fig. 2

Functional analysis of the DEGs.KEGG pathway classification of DEGs in BS and CO.
Functional analysis of the DEGs.KEGG pathway classification of DEGs in BS and CO.

Fig. 2

Functional analysis of the DEGs.KEGG pathway enrichment analysis of DEGs in BS and CO. The abscissa represented the enrichment score. A more significant enrichment score indicates a greater degree of enrichment. The p-value indicates the significantly enriched, and the size of the circle indicates the number of the target genes.
Functional analysis of the DEGs.KEGG pathway enrichment analysis of DEGs in BS and CO. The abscissa represented the enrichment score. A more significant enrichment score indicates a greater degree of enrichment. The p-value indicates the significantly enriched, and the size of the circle indicates the number of the target genes.

Fig. 3

Overview of the differentially expressed milRNAs (DEMs) in BS and CO.A) and B) The DEMs distribution.
Overview of the differentially expressed milRNAs (DEMs) in BS and CO.A) and B) The DEMs distribution.

Fig. 3

Overview of the differentially expressed milRNAs (DEMs) in BS and CO.C) The length distribution of milRNAs in six libraries.
Overview of the differentially expressed milRNAs (DEMs) in BS and CO.C) The length distribution of milRNAs in six libraries.

Fig. 4

GO classification analysis of the target genes of milRNAs between BS and CO in M. acridum.
GO classification analysis of the target genes of milRNAs between BS and CO in M. acridum.

Fig. 5

KEGG enrichment analysis of the target genes of milRNAs between BS and CO in M. acridum. The abscissa represented the enrichment score. A more significant enrichment score indicates a greater degree of enrichment. The p-value indicates the significantly enriched, and the size of the circle indicates the number of the target genes.
KEGG enrichment analysis of the target genes of milRNAs between BS and CO in M. acridum. The abscissa represented the enrichment score. A more significant enrichment score indicates a greater degree of enrichment. The p-value indicates the significantly enriched, and the size of the circle indicates the number of the target genes.

Fig. 6

The relations of the differentially expressed milRNAs and target genes. The color indicated a differentially expressed levels in BS vs. CO for milRNAs and target genes: red indicates upregulated and blue indicates downregulated.
The relations of the differentially expressed milRNAs and target genes. The color indicated a differentially expressed levels in BS vs. CO for milRNAs and target genes: red indicates upregulated and blue indicates downregulated.

Fig. 7

Real-time PCR validation of several DEMs and DEGs.
Real-time PCR validation of several DEMs and DEGs.

Summary of mRNA sequencing reads from BS and CO in M_ acridum_

TreatmentRaw readsClean readsClean basesQ30 (%)GC (%)Mapped reads
BS198,843,64195,572,95614.02G94.06%47.09%67,073,032 (70.18%)
BS298,842,41795,807,41614.02G94.30%46.33%63,815,312 (66.61%)
BS398,841,86995,719,53813.98G94.28%45.60%62,158,068 (64.94%)
CO191,944,12690,288,98413.30G95.74%48.16%68,087,397 (75.41%)
CO298,843,19697,087,78014.31G95.79%47.56%71,191,515 (73.33%)
CO394,432,56892,631,32813.62G95.68%46.99%666,88,341 (71.99%)

Summary of small RNA sequencing and annotation from BS and CO in M_ acridum_

BS1BS2BS3CO1CO2CO3
Raw reads13,988,89711,502,14512,788,57114,441,95014,456,83114,449,135
Clean reads11,994,11010,092,32310,846,88610,825,97812232,89411,080,402
Mapped sRNA reads5,691,2104,397,1984,929,9245,365,3535,761,4155,133,510
Known milRNA numbers8288257651,4411,479798
Novel milRNA numbers231619121414
rRNA numbers8,7759,2715,9499,2755,2247,396
tRNA numbers1,5841,5151,3251,7399771,180
snRNA numbers24,07817,11720,74025,58110,3681,5410
Cis-reg numbers6,4253,6624,1416,5075,2284,408
Other Rfam RNA numbers8,8468,06511,41412,4708,7068,757
Unannotation reads10,495,5199,025,6529,407,6129096,96211,074,9429,625,519
DOI: https://doi.org/10.33073/pjm-2022-028 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 309 - 324
Submitted on: Mar 7, 2022
Accepted on: Jul 1, 2022
Published on: Sep 24, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
blastospore,
morphology,
milRNA,
target gene
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Erhao Zhang, Jie Zhang, Rundong Zhao, Yazhou Lu, Xiu Yin, Xiaozhong Lan, Zhang Luo, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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