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The Component and Functional Pathways of Gut Microbiota Are Altered in Populations with Poor Sleep Quality – A Preliminary Report Cover

The Component and Functional Pathways of Gut Microbiota Are Altered in Populations with Poor Sleep Quality – A Preliminary Report

Polish Journal of Microbiology
Volume 71 (2022): Issue 2 (June 2022)
By: Jianghui Zhang,  Xueqing Zhang,  Kexin Zhang,  Xiaoyan Lu,  Guojing Yuan,  Huayu Yang,  Haiyun Guo,  Zhihui Zhu,  Tianli Wang,  Jiahu Hao,  Ying Sun,  Puyu Su and  Zhihua Zhang  
Open Access
|Jun 2022

Figures & Tables

Fig. 1

Comparison of gut microbiota’s alpha diversity indices between the SD and HC groups, including Observed, Chao 1, ACE, Shannon, Simpson, and Coverage. Plotted in the graphics are the interquartile ranges and boxes, medians (lines in the box), and the lowest and highest values for the first and third quartiles. The abscissa represents a different group; the ordinate represents the value of each diversity index. Different colors distinguish different groups.

Fig. 2

The boxplot chart shows the beta diversity of the bacterial communities from the two groups, based on the Jaccard (A) and Bray-Curtis (B) distances, respectively. The interquartile ranges and boxes, medians (lines in the box), and the lowest and highest values for the first and third quartiles are plotted in the graph. Colors identify each group, and a black dot represents each sample.

Fig. 3

Different color dots represent different groups; the horizontal and vertical axis scale is the relative distance without a practical significance; X-variable 1 and variable 2 represent the putative factors influencing changes in the microbial composition of two groups of samples, respectively. The plot is based on the weighted Unifrac distance.

Fig. 4

The bacterial taxa’s linear discriminant analysis (LDA) effect size (LEfSe). LEfSe plot shows the top ten species with the smallest p-value for each group. Ten bacterial taxa were enriched in the SD group (green), and six bacterial taxa were enriched in the HC group (blue).

Fig. 5

Each point represents a sample and the 95% confidence intervals of the correlation coefficients are shown by grey areas. The relative abundances of genus Holdemanella in the samples were 0.

Fig. 6

Functional prediction analysis of the gut microbiota in the SD and HC groups. Each color represents one group. The bar graph represents the pathways with a significant difference in relative abundance between the two groups. The figure on the right shows 95.0% confidence intervals and p-value.

Correlation analysis of the selected bacterial species with the PSQI score_

LevelTaxon nameRelative abundance (%)q-valuer
PhylumElusimicrobia0.171 ± 0.9950.007*–0.108
PhylumTenericutes0.123 ± 0.6310.030*–0.183
ClassElusimicrobia0.171 ± 0.9950.023*–0.108
ClassMollicutes0.123 ± 0.6310.069–0.183
ClassErysipelotrichia2.606 ± 2.6040.276  0.384*
OrderElusimicrobiales0.171 ± 0.9950.014*–0.108
OrderAnaeroplasmatales0.123 ± 0.6310.058–0.183
OrderDesulfovibrionales0.303 ± 0.2590.188  0.328
OrderErysipelotrichales2.606 ± 2.6040.208  0.384
FamilyElusimicrobiaceae0.171 ± 0.9950.027*–0.108
FamilyAnaeroplasmataceae0.123 ± 0.6310.110–0.183
FamilyDesulfovibrionaceae0.303 ± 0.2590.286  0.328
FamilyErysipelotrichaceae2.606 ± 2.6040.339  0.384*
GenusHoldemanella0.647 ± 1.4900.033*  0.601**
GenusElusimicrobium0.171 ± 0.9950.033*–0.108
GenusAsteroleplasma0.123 ± 0.6310.159–0.183
Speciesuncultured_proteobacterium0.128 ± 0.7480.035*–0.183
Speciesuncultured_bacterium3.700 ± 2.8100.707  0.323

Demographic characteristics of the participants_

CharacteristicsSD (n = 17)HC (n = 17) t / χ 2 $\mathrm{t} / \chi^{2}$p-value
Male/Female10/77/100.1590.303
Age (years)20.059 ± 0.78519.662 ± 0.6111.6460.109
BMI (kg/m2)21.229 ± 2.19420.167 ± 2.1721.4190.166
PSQI score  9.059 ± 1.144  5.118 ± 1.5768.342< 0.001
DOI: https://doi.org/10.33073/pjm-2022-021 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 241 - 250
Submitted on: Dec 14, 2021
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Accepted on: Apr 5, 2022
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Published on: Jun 19, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
sleep disorder,
poor sleep quality,
gut microbiota,
16S rRNA,
college students
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Jianghui Zhang, Xueqing Zhang, Kexin Zhang, Xiaoyan Lu, Guojing Yuan, Huayu Yang, Haiyun Guo, Zhihui Zhu, Tianli Wang, Jiahu Hao, Ying Sun, Puyu Su, Zhihua Zhang, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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