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Oral Microbiota, a Potential Determinant for the Treatment Efficacy of Gastric Helicobacter pylori Eradication in Humans Cover

Oral Microbiota, a Potential Determinant for the Treatment Efficacy of Gastric Helicobacter pylori Eradication in Humans

Polish Journal of Microbiology
Volume 71 (2022): Issue 2 (June 2022)
By: Huixia Chen,  Hui Xie,  Dong Shao,  Liju Chen,  Siyu Chen,  Lin Wang and  Xiao Han  
Open Access
|May 2022

Figures & Tables

Fig. 1

Sample collection and qualification of 16S rRNA sequencing.A) A schematic diagram illustrating the sample collection process; B) rarefaction curve analysis; C) specaccum curve analyses; D) rank abundance analysis; n = 7 for the SE group, n = 9 for the FE group; FE – failure eradication, SE – successful eradication, HP – Helicobacter pylori.

Fig. 2

α and β Diversity analyses.A) Observed species, Chao1 richness, ACE index, Shannon index, Simpson index, and J index were calculated for α diversity of the oral microbiota.

Fig. 2

α and β Diversity analyses.B) PCoA analysis for β diversity of the oral microbiota; SE – patients with successful eradication therapy, FE – patients with failed eradication therapy.

Fig. 3

Component analysis of community structure.A) Relative abundances of the ten most abundant bacterial phyla in the FE and SE groups; B) relative abundances of the indicated individuals’ ten most abundant bacterial phyla; C) t-test analysis comparing oral microbiota composition between the FE and SE groups.

Fig. 3

Component analysis of community structure.D) cladogram generated from LEfSe analysis.

Fig. 3

Component analysis of community structure.E) LDA scores of significantly altered taxa presented in panel D; n = 7 for the SE group, n = 9 for the FE group; SE – patients with successful eradication therapy, FE – patients with failed eradication therapy.

Fig. 4

Correlation analysis of microbial environmental factors.A) RDA analysis comparing OTUs between the FE and SE groups.

Fig. 4

Correlation analysis of microbial environmental factors.E) KEGG analysis diagram; n = 7 for the SE group, n = 9 for the FE group; SE – patients with successful eradication therapy, FE – patients with failed eradication therapy.

Fig. 4

Correlation analysis of microbial environmental factors.B) heat map analysis based on RDA analysis showing the abundance of 14 OTUs influenced by Helicobacter pylori infection.

Fig. 4

Correlation analysis of microbial environmental factors.C) correlations of the abundances of 14 OTUs with DOB values, ages, sex, and treatment status in patients from either FE or SE groups;*p < 0.05, **p < 0.01; PICRUSt2 function prediction of changed OTUs; D) PCA analysis.

Fig. 5

Venn analysis revealing differently affected and overlapping OTUs between the FE and SE groups; n = 7 for the SE group, n = 9 for the FE group; SE – patients with successful eradication therapy, FE – patients with failed eradication therapy.
DOI: https://doi.org/10.33073/pjm-2022-020 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 227 - 239
Submitted on: Jan 5, 2022
|
Accepted on: Mar 22, 2022
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Published on: May 31, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
eradication,
therapeutic effects,
oral microbiota,
sequencing
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Huixia Chen, Hui Xie, Dong Shao, Liju Chen, Siyu Chen, Lin Wang, Xiao Han, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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