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Analysis of Gut Microbiota in Patients with Breast Cancer and Benign Breast Lesions Cover

Analysis of Gut Microbiota in Patients with Breast Cancer and Benign Breast Lesions

Polish Journal of Microbiology
Volume 71 (2022): Issue 2 (June 2022)
By: Zhijun Ma,  Manli Qu and  Xiaowu Wang  
Open Access
|May 2022

Figures & Tables

Fig. 1

The Petaline graph for calculated OTUs. Different colors designate different groups. The central circular area designates the set of OTUs often present in the counterpart groups, and the single-layer zone designates the number of OTUs uniquely found in each group; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.
The Petaline graph for calculated OTUs. Different colors designate different groups. The central circular area designates the set of OTUs often present in the counterpart groups, and the single-layer zone designates the number of OTUs uniquely found in each group; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.

Fig. 2

Comparison of relative taxa richness among breast cancer patients, benign breast lesion patients, and healthy controls. A) Comparison at the phylum level; B) comparison at the genus level; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.
Comparison of relative taxa richness among breast cancer patients, benign breast lesion patients, and healthy controls. A) Comparison at the phylum level; B) comparison at the genus level; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.

Fig. 3

Alpha diversity metrics (Sobs and Chao1 index).A, B) Boxplots for species richness between breast cancer patients and healthy controls; C, D) boxplots for species richness between benign breast lesion patients and healthy controls; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.
Alpha diversity metrics (Sobs and Chao1 index).A, B) Boxplots for species richness between breast cancer patients and healthy controls; C, D) boxplots for species richness between benign breast lesion patients and healthy controls; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.

Fig. 4

Beta diversity assessment based on unweighted and weighted UniFrac.A) Boxplots showing the comparison of beta diversity based on unweighted UniFrac among groups; B) boxplots showing the comparison of beta diversity based on weighted UniFrac among groups; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.
Beta diversity assessment based on unweighted and weighted UniFrac.A) Boxplots showing the comparison of beta diversity based on unweighted UniFrac among groups; B) boxplots showing the comparison of beta diversity based on weighted UniFrac among groups; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.

Fig. 5

PCoA and PLS-DA analysis of microbiota among breast cancer patients, benign breast lesion patients, and healthy controls. Blue circles, orange triangles, and green diamonds represent samples in different groups. The closer the spatial distance of the sample, the more similar the species composition of the sample.A) PCoA plot based on weighted Unifrac; B) PLS-DA plot; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.
PCoA and PLS-DA analysis of microbiota among breast cancer patients, benign breast lesion patients, and healthy controls. Blue circles, orange triangles, and green diamonds represent samples in different groups. The closer the spatial distance of the sample, the more similar the species composition of the sample.A) PCoA plot based on weighted Unifrac; B) PLS-DA plot; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.

Fig. 6

Characteristics of bacterial community composition in breast cancer patients, benign breast lesion patients, and healthy control groups. The linear discriminant analysis (LDA) coupled with effect size (LEfSe) was performed using the LEfSe program. An LDA (log10) score of > 2.0 was considered significant; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.
Characteristics of bacterial community composition in breast cancer patients, benign breast lesion patients, and healthy control groups. The linear discriminant analysis (LDA) coupled with effect size (LEfSe) was performed using the LEfSe program. An LDA (log10) score of > 2.0 was considered significant; BC – breast cancer, BL – benign breast lesions, HC – healthy controls.

Changes in bacterial abundance at the genus level in patients with breast cancer and benign breast lesions_

Breast cancerBenign breast lesions
More abundant generaLess abundant generaMore abundant generaLess abundant genera
EscherichiaFaecalibacteriumEscherichiaCollinsella
PeptoniphilusLachnospiracea_incertae_sedisPeptoniphilusAlistipes
BilophilaCollinsellaCoprobacillusMegamonas
LactobacillusAlistipesLactobacillusButyricimonas
PorphyromonasAnaerofilumPorphyromonasAcidaminococcus
Christensenella Asaccharobacter
Butyricimonas Tissierella
Erysipelothrix Cloacibacillus
Acidaminococcus
Victivallis
Eubacterium
Tissierella
Hydrogenoanaerobacterium
Cloacibacillus
Oxalobacter

Baseline characteristics of the patients enrolled_

Healthy controlsBreast cancerBenign breast lesions
No. of individuals202620
Gender (male/female)0/200/260/20
Mean age (± SD, years)46.90 (10.87)49.62 (7.33)48.95 (8.73)
Mean BMI (± SD, kg/m2)22.80 (2.02)22.88 (1.98)21.71 (2.20)
DOI: https://doi.org/10.33073/pjm-2022-019 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 217 - 226
Submitted on: Nov 4, 2021
Accepted on: Mar 21, 2022
Published on: May 31, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
gut microbiota,
16S rRNA gene sequencing,
breast cancer,
benign breast lesions
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Zhijun Ma, Manli Qu, Xiaowu Wang, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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