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Comparative Analysis of the Microbiota Between Rumen and Duodenum of Twin Lambs Based on Diets of Ceratoides or Alfalfa

Polish Journal of Microbiology
Volume 70 (2021): Issue 2 (June 2021)
By:
Open Access
|Dec 2021

Figures & Tables

Fig. 1.

This figure shows a comparison between the phylum composition of the bacteria in the rumen and duodenum of twin lambs. The letters A1-D1 represent HFLP group, and A2-D2 represent LFHP group.
This figure shows a comparison between the phylum composition of the bacteria in the rumen and duodenum of twin lambs. The letters A1-D1 represent HFLP group, and A2-D2 represent LFHP group.

Fig. 2.

The phylum composition of rumen and duodenum of twin lambs. The letters A1-D1 represent the HFLP group, and the A2-D2 represent the LFHP group of phylum and duodenum, respectively.
The phylum composition of rumen and duodenum of twin lambs. The letters A1-D1 represent the HFLP group, and the A2-D2 represent the LFHP group of phylum and duodenum, respectively.

Fig. 3.

The genus community distribution of rumen and duodenum.a) The genus community distribution of rumen. The letters A1-D1 represent the first group fed with HFLP and A2-D2 represents the second group of twin lambs fed with LFHP in the genus. b) The genus community distribution of duodenum. The letters A1-D1 represent the first group fed with HFLP and A2-D2 represents the second group of twin lambs fed with LFHP in the genus.
The genus community distribution of rumen and duodenum.a) The genus community distribution of rumen. The letters A1-D1 represent the first group fed with HFLP and A2-D2 represents the second group of twin lambs fed with LFHP in the genus. b) The genus community distribution of duodenum. The letters A1-D1 represent the first group fed with HFLP and A2-D2 represents the second group of twin lambs fed with LFHP in the genus.

Fig. 4.

Comparison between HFLP and LFHP’s microbiota and their F:B ratio in the rumen and duodenum.The letters a) and b) represent HFLP pellets and LFHP pellets, respectively, of the rumen. Enterotypes were strongly associated with feeds a) and b) which show LFHP pellets (Bacteroidetes) against HFLP pellets (Firmicutes). LFHP pellets displayed a substantial increase in Bacteroidetes and a reduction in Firmicutes. The letters c) and d) represent HFLP and LFHP pellets, respectively, of the duodenum. Enterotypes were strongly associated with feeds c) and d) which show LFHP pellets (Bacteroidetes) against HFLP pellets (Firmicutes). HFLP pellets displayed an increase in Firmicutes and a reduction in Bacteroidetes.
Comparison between HFLP and LFHP’s microbiota and their F:B ratio in the rumen and duodenum.The letters a) and b) represent HFLP pellets and LFHP pellets, respectively, of the rumen. Enterotypes were strongly associated with feeds a) and b) which show LFHP pellets (Bacteroidetes) against HFLP pellets (Firmicutes). LFHP pellets displayed a substantial increase in Bacteroidetes and a reduction in Firmicutes. The letters c) and d) represent HFLP and LFHP pellets, respectively, of the duodenum. Enterotypes were strongly associated with feeds c) and d) which show LFHP pellets (Bacteroidetes) against HFLP pellets (Firmicutes). HFLP pellets displayed an increase in Firmicutes and a reduction in Bacteroidetes.

Fig. 5.

PLS-DA score plot came from liquid chromatography combined with mass spectrometry (LC/MS) spectra for the HFLP group (blue dots) and the LFHP group (red dots).
PLS-DA score plot came from liquid chromatography combined with mass spectrometry (LC/MS) spectra for the HFLP group (blue dots) and the LFHP group (red dots).

Fig. 6.

Heat map built by the 407 significantly different metabolites (p-value ≤ 0.05) in the HFLP and LFHP groups. The red color represents the LFHP group, and the blue represents the HFLP group.
Heat map built by the 407 significantly different metabolites (p-value ≤ 0.05) in the HFLP and LFHP groups. The red color represents the LFHP group, and the blue represents the HFLP group.

Fig. 7.

The relationship between the two feed (LFHP and HFLP) groups and the metabolites.
The relationship between the two feed (LFHP and HFLP) groups and the metabolites.

Fig. 8.

Metabolites correlation heat map. The rows and columns in the figure represent different metabolites, and different colors represent different correlations. The red color represents a positive correlation, and the blue represents a negative correlation among the metabolites. The deeper the color in the heat map, the better the metabolism.
Metabolites correlation heat map. The rows and columns in the figure represent different metabolites, and different colors represent different correlations. The red color represents a positive correlation, and the blue represents a negative correlation among the metabolites. The deeper the color in the heat map, the better the metabolism.

Fig. 9.

This figure shows the relationship between OTUs, metabolites, and the microbiota. The nodes represent different OTUs related to bacteria, which belong to Firmicutes, Bacteriodetes, and other phyla. The orange diamonds indicate 16 metabolites detected. The lines indicate the correlations between the OTUs, bacteria, and metabolites. The solid red connecting lines show a positive correlation, and the blue connecting dot line indicates a negative correlation between the OTUs, bacteria, and metabolites. The line thickness shows how strong the correlation was.
This figure shows the relationship between OTUs, metabolites, and the microbiota. The nodes represent different OTUs related to bacteria, which belong to Firmicutes, Bacteriodetes, and other phyla. The orange diamonds indicate 16 metabolites detected. The lines indicate the correlations between the OTUs, bacteria, and metabolites. The solid red connecting lines show a positive correlation, and the blue connecting dot line indicates a negative correlation between the OTUs, bacteria, and metabolites. The line thickness shows how strong the correlation was.

The diversity analysis of the rumen microbiota of twin lambs_

ItemsHFLPLFHPp-value
Shannon5.055 ± 0.15894.235 ± 0.1810.014
Simpsons0.019 ± 0.0040.066 ± 0.0410.116
Observed species (Obs)992.000 ± 68.474930.000 ± 18.0370.154
Ace1051.658 ± 64.182992.341 ± 26.8750.102
Chao 11059.470 ± 67.9201017.360 ± 29.3990.165

Nutritional components of two feed stuffs_

DM (%)GE (MJ/kg DM)CP (% DM)CF (% DM)ADF (% DM)NDF (% DM)
LFHP89.416.316.12.425.246.2
HFLP90.515.811.82.229.657.5

The diversity analysis of duodenum microbiota of twin lambs_

ItemsHFLPLFHPp-value
Shannon7.855 ± 0.8807.828 ± 0.8380.964
Simpsons0.985 ± 0.0060.986 ± 0.0050.391
Observed species (Obs)966.750 ± 293.842905.000 ± 247.8180.781
Chao 11278.063 ± 241.6781223.815 ± 327.1980.843
DOI: https://doi.org/10.33073/pjm-2021-015 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Journal RSS Feed
Language: English
Page range: 175 - 187
Submitted on: Nov 21, 2020
Accepted on: Mar 6, 2021
Published on: Dec 6, 2021
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
correlation analysis,
digesta,
metabolome,
16S rRNA sequencing,
sheep
Related subjects:
Life sciences,
Microbiology and virology

© 2021 ZACCHEAUS PAZAMILALA AKONYANI, FENG SONG, YING LI, SUDE QIQIGE, JIANGHONG WU, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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