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Fig. 6.
The specific primers of Leptospira_
| Name of primer | Type | Sequence of primer |
|---|---|---|
| 16S rRNA | Forward | 5’-GAACTGAGACACGGTCCAT-3’ |
| 16S rRNA | Reverse | 5’-GCCTCAGCGTCAGTTTTAGG-3’ |
| LipL32 | Forward | 5’-ATCTCCGTTGCACTCTTTGC-3’ |
| LipL32 | Reverse | 5’-ACCATCATCATCATCGTCCA-3’ |
The results of the sequencing_
| Molecular detection | Max Score | Total Score | Query Cover | E Value | Per Ident | Accession |
|---|---|---|---|---|---|---|
| Leptospira perdikensis strain CES 16S ribosomal RNA gene | 612 | 612 | 93% | 3e-171 | 99.41% | MN086353.1 |
| Leptospira ryugenii strain CES 16S ribosomal RNA gene | 176 | 176 | 26% | 5e-40 | 100% | MN086356.1 |
| Leptospira sp. MS336 gene for 16S ribosomal RNA gene | 593 | 593 | 100% | 2e-166 | 100% | AB758752.1 |
The sampling sites and positive cases reported in the study_
| Number of positive cases of saprophytic Leptospira | Number of positive cases of pathogenic Leptospira | Number of samples | Site |
|---|---|---|---|
| 3 | 2 | 5 | Valiabad |
| 2 | 2 | 5 | Bagh e nazar |
| 0 | 1 | 5 | Mir shams-ol |
| 1 | 1 | 5 | Rud e posht |
| 1 | 0 | 5 | Alkaleh |
| 1 | 0 | 5 | Abkele sar |
| 0 | 1 | 5 | Lashkarak |
| 1 | 1 | 5 | Nematabad |
| 1 | 1 | 5 | Shiraj mahalleh-ye bozorg |
| 1 | 1 | 5 | Tilpordehsar |
| 11 (22%) | 10 (20%) | 50 | Total |