Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
j_pjm-2020-013_tab_006
| Taxonomy | I.DNF | CRI.DNF | E.DNF | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Top1 (%) | Top2 (%) | Top3 (%) | Top1 (%) | Top2 (%) | Top3 (%) | Top1 (%) | Top2 (%) | Top3 (%) | |
| Phylum | Proteobacteria (81.7) | Bacteroidetes (18.1) | Firmicutes (0.05) | Proteobacteria (99.5) | Bacteroidetes (0.4) | Actinobacteria (0.07) | Proteobacteria (74.9) | Bacteroidetes (25.0) | Firmicutes (0.05) |
| Class | Gammaproteobacteria (80.4) | Bacteroidia (18.1) | Alphaproteobacteria (1.2) | Alphaproteobacteria (60.9) | Gammaproteobacteria (38.6) | Bacteroidia (0.39) | Gammaproteobacteria (72.9) | Bacteroidia (25.0) | Alphaproteobacteria (1.9) |
| Order | Pseudomonadales (56.5) | Flavobacteriales (17.0) | Aeromonadales (11.6) | Rhizobiales (60.5) | Pseudomonadales (24.5) | Aeromonadales (7.7) | Aeromonadales (29.6) | Flavobacteriales (24.1) | Pseudomonadales (17.1) |
| Family | Pseudomonadaceae (35.1) | Moraxellaceae (21.4) | Flavobacteriaceae (16.7) | Rhizobiaceae (60.5) | Moraxellaceae (14.3) | Pseudomonadaceae (10.2) | Aeromonadaceae (25.1) | unidentified_Flavobacteriales (21.5) | Moraxellaceae (14.3) |
| Genus | Pseudomonas (34.8) | Acinetobacter (20.3) | Flavobacterium (16.7) | unidentified_Rhizobiaceae (61.4) | Acinetobacter (12.7) | Pseudomonas (6.1) | Aeromonas (22.5) | Cloacibacterium (20.9) | Acinetobacter (16.8) |
Sample names and processing methods in the study_
| Sample source | Processing methods | Sample names |
|---|---|---|
| STE Influent Water | Filtration of STE influent water samples with filter membrane | IC |
| Filtration of eluate from OHN medium | I.NF | |
| Filtration of eluate from OAD medium | I.DNF | |
| CRI Effluent Water | Filtration of CRI effluent water samples with filter membrane | CRIC |
| Filtration of eluate from OHN medium | CRI.NF | |
| Filtration of eluate from OAD medium | CRI.DNF | |
| STE Effluent Water | Filtration of STE effluent water samples with filter membrane | EC |
| Filtration of eluate from OHN medium | E.NF | |
| Filtration of eluate from OAD medium | E.DNF |
Physical and chemical parameters of the STE influent, CRI effluent, and STE effluent water samples_
| Sample Name | DO (mg/l) | Turbidity (NTU) | Conductivity (S/m) | pH | CODMn (mg/l) | TN (mg/l) | no3 --n (mg/l) | nh4 +–n (mg/l) | TP (mg/l) |
|---|---|---|---|---|---|---|---|---|---|
| IC | 1.47 ± 0.0 | 16.00 ± 3.3 | 0.98 ± 0.0 | 7.83 ± 0.0 | 17.46 ± 1.6 | 19.4 ± 0.0 | 0.98 ± 0.0 | 16.6 ± 0.5 | 0.92 ± 0.0 |
| CRIC | 3.92 ± 0.1 | 0.27 ± 0.2 | 0.79 ± 0.0 | 6.96 ± 0.3 | 6.46 ± 0.1 | 2.36 ± 0.1 | 0.72 ± 0.0 | 0.64 ± 0.0 | 0.30 ± 0.0 |
| EC | 5.37 ± 0.1 | 0.27 ± 0.2 | 0.77 ± 0.0 | 7.59 ± 0.0 | 4.98 ± 0.2 | 0.31 ± 0.0 | 0.10 ± 0.0 | 0.10 ± 0.0 | 0.22 ± 0.3 |
| ANOVA | *** | ** | *** | *** | *** | *** | *** | *** | *** |
j_pjm-2020-013_tab_005
| Taxonomy | I.NF | CRI.NF | E.NF | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Top1 (%) | Top2 (%) | Top3 (%) | Top1 (%) | Top2 (%) | Top3 (%) | Top1 (%) | Top2 (%) | Top3 (%) | |
| Phylum | Proteobacteria (79.0) | Bacteroidetes (20.6) | Firmicutes (0.09) | Proteobacteria (96.6) | Bacteroidetes (2.9) | Actinobacteria (0.12) | Proteobacteria (75.4) | Bacteroidetes (24.5) | Firmicutes (0.06) |
| Class | Gammaproteobacteria (77.0) | Bacteroidia (20.6) | Alphaproteobacteria (2.0) | Gammaproteobacteria (66.2) | Alphaproteobacteria (30.3) | Bacteroidia (2.9) | Gammaproteobacteria (73.8) | Bacteroidia (24.5) | Alphaproteobacteria (1.6) |
| Order | Pseudomonadales (61.4) | unidentified_Gammaproteobacteria (6.7) | Aeromonadales (5.7) | Pseudomonadales (42.7) | Rhizobiales (28.6) | Aeromonadales (11.5) | Pseudomonadales (35.6) | Flavobacteriales (24.0) | unidentified_Gammaproteobacteria (18.3) |
| Family | Moraxellaceae (45.7) | Flavobacteriaceae (19.1) | Pseudomonadaceae (15.8) | Moraxellaceae (32.8) | Rhizobiaceae (28.7) | Xanthomonadaceae (10.5) | Moraxellaceae (31.9) | unidentified_Flavobacteriales (20.6) | Burkholderiaceae (9.9) |
| Genus | Acinetobacter (46.7) | Flavobacterium (20.5) | Pseudomonas (15.8) | Acinetobacter (32.1) | unidentified_Rhizobiaceae (28.5) | Pseudomonas (10.5) | Acinetobacter (31.9) | unidentified_Rhizobiaceae (22.5) | Aeromonas (20.6) |
Effective reads, OTUs, and richness estimators (Chao1 and ACE) of nine samples_
| Sample Names | Effective reads | OTUs | Chao1 | ACE |
|---|---|---|---|---|
| IC | 46433 | 171 | 161.5 | 163.5 |
| I.NF | 47463 | 142 | 161.0 | 158.9 |
| I.DNF | 80100 | 137 | 139.3 | 141.9 |
| CRIC | 54424 | 131 | 132.6 | 137.9 |
| CRI.NF | 59932 | 118 | 117.8 | 126.0 |
| CRI.DNF | 64800 | 103 | 123.9 | 135.2 |
| EC | 48924 | 88 | 85.2 | 89.7 |
| E.NF | 50389 | 132 | 128.3 | 130.5 |
| E.DNF | 56999 | 125 | 121.0 | 122.4 |
The relative abundance of the top three populations at different levels of the taxonomy_
| Taxonomy | IC | CRIC | EC | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Top1 (%) | Top2 (%) | Top3 (%) | Top1 (%) | Top2 (%) | Top3 (%) | Top1 (%) | Top2 (%) | Top3 (%) | |
| Phylum | Proteobacteria (96.6) | Firmicutes (17.4) | Bacteroidetes (0.96) | Proteobacteria (99.3) | Firmicutes (0.17) | Bacteroidetes (0.16) | Proteobacteria (99.7) | Bacteroidetes (0.14) | Firmicutes (0.09) |
| Class | Gammaproteobacteria (83.8) | Alphaproteobacteria (12.8) | Clostridia (0.97) | Gammaproteobacteria (83.7) | Alphaproteobacteria (15.6) | Bacteroidia (0.16) | Gammaproteobacteria (81.7) | Alphaproteobacteria (18.0) | Bacteroidia (0.13) |
| Order | Xanthomonadales (78.0) | Sphingomonadales (6.1) | Rhizobiales (5.2) | Xanthomonadales (72.7) | Rhizobiales (7.8) | Pseudomonadales (5.8) | Xanthomonadales (72.7) | Rhizobiales (7.8) | Sphingomonadales (5.8) |
| Family | Xanthomonadaceae (78.0) | Rhizobiaceae (4.9) | Others (11.4) | Xanthomonadaceae (72.6) | Others (8.5) | Rhizobiaceae (7.8) | Xanthomonadaceae (72.7) | Rhizobiaceae (7.8) | Others (8.5) |
| Genus | Stenotrophomonas (78.0) | Sphingomomas (12.1) | Phyllobacterium (4.7) | Stenotrophomonas (72.7) | Phyllobacterium (10.0) | Sphingomomas (7.5) | Stenotrophomonas (72.6) | Sphingomomas (9.5) | Delftia (9.1) |