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Effects of Sodium Tripolyphosphate on Oral Commensal and Pathogenic Bacteria

Polish Journal of Microbiology
Volume 68 (2019): Issue 2 (June 2019)
By:
Open Access
|Jun 2019

Figures & Tables

Fig. 1.

MIC determination of polyP3 by the agar dilution method. The bacterial cells were spot-inoculated (approximately 105 to 106 cells/spot) onto Brucella blood agar plates containing polyP3 at various concentrations and incubated at 37°C for 3 days anaerobically. The MIC was defined as the lowest concentration that inhibited the bacterial growth on the plate. The results for P. intermedia ATCC 49046 are the same as those reported in our previous study (Jang et al. 2016).
MIC determination of polyP3 by the agar dilution method. The bacterial cells were spot-inoculated (approximately 105 to 106 cells/spot) onto Brucella blood agar plates containing polyP3 at various concentrations and incubated at 37°C for 3 days anaerobically. The MIC was defined as the lowest concentration that inhibited the bacterial growth on the plate. The results for P. intermedia ATCC 49046 are the same as those reported in our previous study (Jang et al. 2016).

Fig. 2.

Effect of polyP3 on the growth of the bacteria at a high inoculum density. Each bacterial strain cultured to exponential phase was adjusted to approximately 108 cells/ml in B-HK, then exposed to polyP3 at various concentrations. The bacterial growth was measured by reading optical density at 600 nm (OD600) after the 24-hour incubation. Data are means ± SDs from two independent experiments performed in triplicate. One-way ANOVA with Tukey’s post-hoc tests p < 0.001, versus control (0% polyP3).
Effect of polyP3 on the growth of the bacteria at a high inoculum density. Each bacterial strain cultured to exponential phase was adjusted to approximately 108 cells/ml in B-HK, then exposed to polyP3 at various concentrations. The bacterial growth was measured by reading optical density at 600 nm (OD600) after the 24-hour incubation. Data are means ± SDs from two independent experiments performed in triplicate. One-way ANOVA with Tukey’s post-hoc tests p < 0.001, versus control (0% polyP3).

Fig. 3.

Effect of polyP3 on the growth and biofilm formation of the four bacterial species in a mixed culture. (A) To assess the total growth (planktonic and biofilm bacterial growth), the biofilm bacterial cells were dispersed, mixed thoroughly with planktonic bacterial cells, and OD600 of the suspension was measured. The suspension was diluted 2 to 10 times, and OD600 of the diluted suspension was also measured. The amount of biofilm formed by the mixed bacterial species was quantitated by crystal violet staining. Readings were expressed as mean %OD600 (%OD600 = OD600 ∕ mean OD600 of control × 100). One-way ANOVA with Tukey’s post-hoc tests p < 0.001, versus control (0% polyP3). (B) SEM images of the biofilms at a magnification of 20 000 × (left) and 100 000 × (right).
Effect of polyP3 on the growth and biofilm formation of the four bacterial species in a mixed culture. (A) To assess the total growth (planktonic and biofilm bacterial growth), the biofilm bacterial cells were dispersed, mixed thoroughly with planktonic bacterial cells, and OD600 of the suspension was measured. The suspension was diluted 2 to 10 times, and OD600 of the diluted suspension was also measured. The amount of biofilm formed by the mixed bacterial species was quantitated by crystal violet staining. Readings were expressed as mean %OD600 (%OD600 = OD600 ∕ mean OD600 of control × 100). One-way ANOVA with Tukey’s post-hoc tests p < 0.001, versus control (0% polyP3). (B) SEM images of the biofilms at a magnification of 20 000 × (left) and 100 000 × (right).
DOI: https://doi.org/10.33073/pjm-2019-029 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 263 - 268
Submitted on: Jan 10, 2019
Accepted on: Apr 22, 2019
Published on: Jun 28, 2019
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
polyphosphate,
antibacterial,
oral bacteria,
commensal,
pathogenic
Related subjects:
Life sciences,
Microbiology and virology

© 2019 JI-HOI MOON, MI HEE NOH, EUN-YOUNG JANG, SEOK BIN YANG, SANG WOOK KANG, KYU HWAN KWACK, JAE-IN RYU, JIN-YONG LEE, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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