Table I
Strains used in this study.
| Strain or plasmid | Genotype / resistance / description | Reference |
|---|---|---|
| Campylobacter jejuni | ||
| 81–176 (ATCC BAA-, 2151) | pVir, TcR (Wild type, human isolate) | |
| 81–176 htrA− | pVir, htrA::aphA3, TcR, KmR | This study |
| 81–176 dsbI− | pVir, cfafolvcaf TcR, CmR | (Raczko et al. 2005) |
Fig. 1.
Comparison of the OMVs production.
The concentration of OMVs, isolated from C. jejuni 81–176 grown under optimal and stress conditions (15% O2 and polymyxin B) and from C. jejuni 81–176 dsbI and htrA mutants, was measured by BCA assay. The concentration of OMVs produced by the strain grown under optimal conditions was marked as 1. A statistical analysis was performed using GraphPad Prism 6 (GraphPad Software). Statistical analysis was carried out using multifactorial (one-way) ANOVA followed by Tukey’s multiple comparisons test. An asterisk indicates significant differences (p < 0.05) between analyzed groups and the control group.
Table II
Up- and downregulated proteins under the polymyxin B and oxidative stress and in the Δdsbl mutant background (the protein numeration of C. jejuni NCTC11168 strain).
| Protein / Gene | Number of peptides | q-value | Fold* |
|---|---|---|---|
| Strain 81–176 wt vs. strain 81–176 grown under oxidative stress conditions | |||
| Major outer membrane protein (porA, Cj1259) | 56 | 0.00038 | +1.96 |
| Flagellin A (flaA, Cj1339c) | 25 | 0.00494 | +2.18 |
| Iron ABC transporter, periplasmic iron-binding protein (Cj0175c) | 29 | 0.00507 | +1.83 |
| Putative lipoprotein (Cj1090c) | 7 | 0.00627 | −4.07 |
| Strain 81–176 wt vs. strain 81–176 grown under the polymyxin B stress | |||
| Flagellin B (flaB, Cj1338c) | 83 | 0.00021 | +4.57 |
| Major outer membrane protein (porA, Cj1259) | 60 | 0.00021 | +9.09 |
| Serine protease, protease DO (htrA, Cj1228c) | 55 | 0.01684 | −3.14 |
| Flagellar hook protein (flgE, Cj1729c) | 34 | 0.019 | +4.67 |
| Putative pyridoxamine 5-phosphate oxidase (Cj1613c) | 7 | 0.08 | +6.16 |
| Strain 81–176 wt vs. strain 81–176 dsbI– | |||
| 60 kDa chaperonin (groL, Cj1221) | 95 | 0.00040 | −4.45 |
| Fagellar hook-associated protein (flgL, Cj0887c) | 20 | 0.003 | +8.81 |
| Enolase (Cj1672c) | 27 | 0.02 | −2.16 |
| Coproporphyrinogen-III oxidase (hemN, Cj0992c) | 10 | 0.038 | +10.68 |
| Flagellar basal body rod protein (flgC, Cj0527c) | 18 | 0.042 | −2,49 |
| Flagellar hook protein (flgE, Cj1729c) | 33 | 0.059 | +2.7 |
| Anthranilate synthase subunit I (trpE, Cj0345) | 18 | 0.06 | −2.7 |
| Fumarate hydratase class II (fumC, Cj1364c) | 34 | 0.09 | −2.3 |
| Strain 81–176 wt vs. strain 81–176 htrA– | |||
| No significant changes noted | |||