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Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni

Open Access
|Jun 2019

Figures & Tables

Fig. 1.

Comparison of the OMVs production.The concentration of OMVs, isolated from C. jejuni 81–176 grown under optimal and stress conditions (15% O2 and polymyxin B) and from C. jejuni 81–176 dsbI and htrA mutants, was measured by BCA assay. The concentration of OMVs produced by the strain grown under optimal conditions was marked as 1. A statistical analysis was performed using GraphPad Prism 6 (GraphPad Software). Statistical analysis was carried out using multifactorial (one-way) ANOVA followed by Tukey’s multiple comparisons test. An asterisk indicates significant differences (p < 0.05) between analyzed groups and the control group.
Comparison of the OMVs production.The concentration of OMVs, isolated from C. jejuni 81–176 grown under optimal and stress conditions (15% O2 and polymyxin B) and from C. jejuni 81–176 dsbI and htrA mutants, was measured by BCA assay. The concentration of OMVs produced by the strain grown under optimal conditions was marked as 1. A statistical analysis was performed using GraphPad Prism 6 (GraphPad Software). Statistical analysis was carried out using multifactorial (one-way) ANOVA followed by Tukey’s multiple comparisons test. An asterisk indicates significant differences (p < 0.05) between analyzed groups and the control group.

Up- and downregulated proteins under the polymyxin B and oxidative stress and in the Δdsbl mutant background (the protein numeration of C_ jejuni NCTC11168 strain)_

Protein / GeneNumber of peptidesq-valueFold*
Strain 81–176 wt vs. strain 81–176 grown under oxidative stress conditions
Major outer membrane protein (porA, Cj1259)560.00038+1.96
Flagellin A (flaA, Cj1339c)250.00494+2.18
Iron ABC transporter, periplasmic iron-binding protein (Cj0175c)290.00507+1.83
Putative lipoprotein (Cj1090c)70.00627−4.07
Strain 81–176 wt vs. strain 81–176 grown under the polymyxin B stress
Flagellin B (flaB, Cj1338c)830.00021+4.57
Major outer membrane protein (porA, Cj1259)600.00021+9.09
Serine protease, protease DO (htrA, Cj1228c)550.01684−3.14
Flagellar hook protein (flgE, Cj1729c)340.019+4.67
Putative pyridoxamine 5-phosphate oxidase (Cj1613c)70.08+6.16
Strain 81–176 wt vs. strain 81–176 dsbI
60 kDa chaperonin (groL, Cj1221)950.00040−4.45
Fagellar hook-associated protein (flgL, Cj0887c)200.003+8.81
Enolase (Cj1672c)270.02−2.16
Coproporphyrinogen-III oxidase (hemN, Cj0992c)100.038+10.68
Flagellar basal body rod protein (flgC, Cj0527c)180.042−2,49
Flagellar hook protein (flgE, Cj1729c)330.059+2.7
Anthranilate synthase subunit I (trpE, Cj0345)180.06−2.7
Fumarate hydratase class II (fumC, Cj1364c)340.09−2.3
Strain 81–176 wt vs. strain 81–176 htrA
No significant changes noted

Strains used in this study_

Strain or plasmidGenotype / resistance / descriptionReference
Campylobacter jejuni
81–176 (ATCC BAA-, 2151)pVir, TcR (Wild type, human isolate) 
81–176 htrApVir, htrA::aphA3, TcR, KmRThis study
81–176 dsbIpVir, cfafolvcaf TcR, CmR(Raczko et al. 2005)
DOI: https://doi.org/10.33073/pjm-2019-027 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 255 - 261
Submitted on: Jan 30, 2019
Accepted on: Apr 12, 2019
Published on: Jun 28, 2019
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year

© 2019 RENATA GODLEWSKA, JOANNA KLIM, JANUSZ DĘBSKI, AGNIESZKA WYSZYŃSKA, ANNA ŁASICA, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.